USE OF NATURAL WASTE CARRIER IN ENZYME IMMOBILIZATION: CATALASE IMMOBILIZATION ONTO EGGSHELL MEMBRANE

Year 2022, Volume: 8 Issue: 2, 70 - 76, 30.12.2022

Ceyhun Işık

https://doi.org/10.22531/muglajsci.1183878

Cited By: 2

Abstract

The immobilization process improves the stability properties of enzymes and reduces production costs in industrial processes due to its reuse feature. In this study, catalase was immobilized onto eggshell membrane (ESM), which is a natural carrier macromolecule, via adsorption and cross-linking methods. Experimental parameters such as enzyme concentration, ESM amount, cross-linking agent concentration and adsorption time were determined. Then, optimization studies were performed for both free catalase and catalase immobilized ESM. It was observed that ESM improved the stability properties of catalase enzyme such as optimum temperature, thermal stability and optimum pH after the immobilization. Especially in alkaline conditions, catalase immobilized ESM showed higher activity than free enzyme. The catalase immobilized ESM was able to maintain more than 50% of its activity even after repeated 25 uses. In addition, characterization studies of ESM and catalase immobilized ESM were performed using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).

Keywords

Catalase, immobilization, adsorption, cross-linking, eggshell membrane

References

• Barbosa, O., Ortiz, C., Berenguer-Murcia, Á., Torres, R., Rodrigues, R.C. and Lafuente, R.F., “Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts”, Biotechnology Advances, 33(5), 435-456, 2015.
• Huang, X.J., Yu, A.G., Jiang, J., Pan, C., Qian, J.W. and Xu, Z.K., “Surface modification of nanofibrous poly(acrylonitrile-co-acrylic acid) membrane with biomacromolecules for lipase immobilization”, Journal of Molecular Catalysis B: Enzymatic, 57(1-4), 250-256, 2009.
• Pasunooti, S., Surya, W., Tan, S.N. and Liang, Z.X., “Sol-gel immobilization of a thermophilic diguanylate cyclase for enzymatic production of cyclic-di-GMP”, Journal of Molecular Catalysis B: Enzymatic, 67(1-2), 98-103, 2010.
• Canbolat, M.H., Savaş, H.B. and Gultekin, F., “Improved catalytic activity by catalase immobilization using γ-cyclodextrin and electrospun PCL nanofibers”, Journal of Applied Polymer Science, 134(4), 44404-44410, 2016.
• Rueda, N., dos Santos, J.C.S., Ortiz, C., Torres, R., Barbosa, O., Rodrigues, R.C., Berenguer-Murcia, Á., and Lafuente, R.F., “Chemical modification in the design of immobilized enzyme biocatalysts: drawbacks and opportunities”, The Chemical Record, 16(3), 1436-1455, 2016.
• Brena, B., Pombo P.G., and Viera, F.B., (Editor, Guisan, J.), Immobilization of Enzymes and Cells. Methods in Molecular Biology, Humana Press, Totowa, 2013.
• Wang, Z.G., Wan, L.S., Liu, Z.M., Huang X.J., and Xu, Z.K., “Enzyme immobilization on electrospun polymer nanofibers: An overview” Journal of Molecular Catalysis B: Enzymatic, 56(4), 189-195, 2009.
• Rodrigues, R.C., Ortiz, C., Berenguer-Murcia, Á., Torres, R. and Lafuente, R.F., “Modifying enzyme activity and selectivity by immobilization”, Chemical Society Reviews, 42, 6290-6307, 2013.
• Datta, S., Christena, L.R., and Rajaram, Y.R.S., “Enzyme immobilization: an overview on techniques and support materials”, 3 Biotech, 3(1), 1-9, 2013.
• Jiang, C., Cheng, C., Hao, M., Wang, H., Wang, Z., Shen C., and Cheong, L.Z., “Enhanced catalytic stability of lipase immobilized on oxidized and disulfide-rich eggshell membrane for esters hydrolysis and transesterification”, International Journal of Biological Macromolecules, 105(1), 1328-1336, 2017.
• Balaz M., “Eggshell membrane biomaterial as a platform for applications in materials science”, Acta Biomaterialia, 10(9), 3827-3843, 2014.
• Işık, C., Saraç, N., Teke, M. and Uğur, A., “A new bioremediation method for removal of wastewater containing oils with high oleic acid composition: Acinetobacter haemolyticus lipase immobilized on eggshell membrane with improved stabilities”, New Journal of Chemistry, 45, 1984-1992, 2021.
• Inanan, T., Tüzmen, N. and Karipcin, F., “Oxime-functionalized cryogel disks for catalase immobilization”, International Journal of Biological Macromolecules, 114, 812-820, 2018.
• Kaushal, J., Singh, G. and Arya, S.K., “Immobilization of catalase onto chitosan and chitosan-bentonite complex: a comparative study”, Biotechnology Reports, 18, e00258, 2018.
• Hussein, A.A., “Purification and characterization of thermo-alkali stable catalase from Bacillus sp”, International Research Journal of Biotechnology, 3(10), 207-214, 2012.
• Alptekin, Ö., Tükel, S.S., Yildirim, D., and Alagöz, D., “Covalent immobilization of catalase onto spacer-arm attached modified florisil: characterization and application to batch and plug-flow type reactor systems”, Enzyme and Microbial Technology, 49(6-7), 547-554, 2011.
• Grigoras, A.G., “Catalase immobilization-a review”, Biochemical Engineering Journal, 117, 1-20 2017.
• Quan, J., Liu, Z., White, C.B., Nie, H. and Zhu, L., “Fabrication of glycopolymer/MWCNTs composite nanofibers and its enzyme immobilization applications”, Colloids and Surfaces B: Biointerfaces, 121, 417-424, 2014.
• Abdelhamidab M.A.A., Meligy A.M.A., Yeo K.B., Lee C.S. and Pack S.P., “Silaffin-3-derived pentalysine cluster as a new fusion tag for one-step immobilization and purification of recombinant Bacillus subtilis catalase on bare silica particles”, International Journal of Biological Macromolecules, 159, 1103-1112, 2020.
• Czechowska, E., Soliwoda, K.R., Tomaszewska, E., Pudlarz, A., Celichowski, G., Zwolenik, D.G., Szemraj, J. and Grobelny, J., "Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption", Colloids and Surfaces B: Biointerfaces, 171, 707-714, 2018.
• Liou, J.H., Wang, Z.H., Chen, I.H., Wang, S.S.S., How, S.C. and Jan, J.S., "Catalase immobilized in polypeptide/silica nanocomposites via emulsion and biomineralization with improved activities", International Journal of Biological Macromolecules, 159, 931-940, 2020.
• Bradford N.M., “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding”, Analytical Biochemistry, 72 (1-2), 248-254, 1976.
• Lartillot, S., Kedziora, P., and Athias, A., “Purification and Characterization of a New Fungal Catalase”, Preparative Biochemistry, 18(3), 241-246, 1988.
• Inanan, T., “Chitosan Co-polymeric nanostructures for catalase immobilization”, Reactive and Functional Polymers, 135, 94-102, 2019.
• Doğaç, Y.İ. and Teke, M. “Immobilization of bovine catalase onto magnetic nanoparticles”, Preparative Biochemistry and Biotechnology, 43(8), 750-765, 2013.
• Işık, C., Arabaci, G., Doğac, Y.I., Deveci, I. and Teke, M., “Synthesis and characterization of electrospun PVA/Zn2+ metal composite nanofibers for lipase immobilization with effective thermal, pH stabilities and reusability”, Materials Science and Engineering C, 99, 1226-1235, 2019.
• Alptekin, O., Tukel, S.S., Yıldırım, D. and Alagoz, D., “Immobilization of Catalase Onto Eupergit C and Its Characterization”, Journal of Molecular Catalysis B: Enzymatic, 64(3-4), 177-183, 2010.
• Tuzmen, N., Kalburcu, T., and Denizli, A., “Immobilization of Catalase Via Adsorption Onto Metal-Chelated Affinity Cryogels”, Process Biochemistry, 47(1), 26-33, 2012.
• Erol, K., Cebeci B.K., Köse K. and Köse, D.A., “Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels”, International Journal of Biological Macromolecules, 123, 738-743, 2019.
• Kaiden, K., Matsui, T. and Tanaka, S., “A Study of the Amide III Band by FT-IR Spectrometry of the Secondary Structure of Albumin, Myoglobin, and γ-Globulin”, Applied Spectroscopy, 41(2), 180-184, 1987.
• Weymuth, T., Jacob, C.R. and Reiher, M., “A Local-Mode Model for Understanding the Dependence of the Extended Amide III Vibrations on Protein Secondary Structure”, The Journal of Physical Chemistry B, 114(32), 10649-10660, 2010.
• Arami, M., Limaee, N.Y., and Mahmoodi, N.M., “Investigation on the adsorption capability of egg shell membrane towards model textile dyes”, Chemosphere, 65(11), 1999-2008, 2006.
• Zhao Y.H., and Chi, Y.J., “Characterization of Collagen from Eggshell Membrane”, Biotechnology, 8(2), 254-258, 2009.