Mo-KAPLI TUNGSTEN-SARMAL ATOM TUZAK SOĞUK BUHAR ATOMİK ABSORPSİYON SPEKTROMETRİSİ KULLANILARAK MUSLUK SUYU VE BALIK KAS DOKUSU ÖRNEKLERİNDEKİ CIVA TAYİNİ İÇİN YENİ BİR TEKNİĞİN GELİŞTİRİLMESİ

Year 2024, Volume: 10 Issue: 1, 152 - 158, 30.06.2024

Muhammet Atasoy

https://doi.org/10.22531/muglajsci.1473671

Abstract

Bu çalışmada, gaz halindeki cıvanın yerinde tuzaklanması için bir kuvars tüp atomlaştırıcı içine yerleştirilen Mo-kaplı tungsten-sarmal (W-sarmal) ile geleneksel soğuk buhar atomik absorpsiyon spektrometresi (CV-AAS) birleştirilerek ultra eser düzeyde toplam cıva (T-Hg) tayini için yeni ve düşük maliyetli bir teknik geliştirildi. Önerilen teknikteki parametrelerin optimum çalışma koşulları, daha düşük analiz seviyelerine inebilmek için deneysel olarak belirlendi. Geliştirilen teknikten elde edilen analitik sonuçlar geleneksel CV-AAS tekniğinden elde edilenlerle karşılaştırıldı. Geliştirilen teknik, karakteristik konsantrasyon (C0) ile ifade edildiği üzere, geleneksel tekniğe kıyasla duyarlılıkta 39,7 katlık bir zenginleştirme elde edildi. Önerilen teknik ile 120 saniyelik bir tuzaklama süresi için tayin sınırı (LOD) 0,017 µg L-1 ve bağıl standart sapma (RSD%) ise %3,6 olarak hesaplandı. Önerilen analitik prosedür, sertifikalı bir referans malzemeler, spike edilmiş musluk suyu örnekleri ve balık kas dokularındaki Hg konsantrasyonlarını belirlemek için kullanıldı. Su numuneleri için geri kazanım değerleri tatmin edici olup %104,9 ile %111,3 arasında değişmekteydi.

Keywords

Cıva Tayini, Musluk suyu numuneleri, Balık numuneleri, Atom tuzak, Mo-kaplı W-sarmal

DEVELOPMENT OF A NEW TECHNIQUE FOR DETERMINATION OF MERCURY IN TAP WATER AND FISH MUSCLE TISSUE SAMPLES USING Mo-COATED TUNGSTEN-COIL ATOM TRAP COLD VAPOR ATOMIC ABSORPTION SPECTROMETRY

Abstract

In this study, a new and low-cost technique for the determination of ultra-trace level of total mercury (T-Hg) was developed by combining a Mo-coated tungsten-coil (W-coil) placed in a quartz tube atomizer for in situ trapping of gaseous mercury with a conventional cold vapor atomic absorption spectrometry (CV-AAS). The optimum operating conditions of the parameters in the proposed technique were determined experimentally in order to achieve lower detection limits. The analytical results obtained from the developed approach were compared with those obtained from the traditional CV-AAS technique. The developed technique demonstrated a significant 39.7-fold improvement in sensitivity compared to the traditional technique, as expressed by the characteristic concentration (C0). The technique described yielded a limit of detection (LOD) of 0.017 µg L-1 and a relative standard deviation (RSD%) of 3.6% for a capture time of 120 s. The proposed analytical procedure was utilized to determine the concentrations of Hg in certified reference materials, spiked tap water samples and fish muscle tissue samples. The recovery values for the water samples were satisfactory, ranging between 104.9% and 111.3%.

Keywords

Mercury determination, Tap water samples, Fish samples, Atom trap, Mo-coated W-coil

References

• Han, C., Zheng, C., Wang, J., Cheng, G., Lv, Y. and Hou, X., “Photo-induced cold vapor generation with low molecular weight alcohol, aldehyde, or carboxylic acid for atomic fluorescence spectrometric determination of mercury”, Analytical and Bioanalytical Chemistry, 388, 825-830, 2007.
• Xia, H., Liu, X., Huang, K., Gao, Y., Gan, L., He, C. and Hou, X., “Matrix-assisted UV-photochemical vapor generation for AFS determination of trace mercury in natural water samples: a green analytical method”, Spectroscopy Letters, 43, 550-554, 2010.
• Yildiz, D., Atasoy, M. and Arslan, Y., “Ultra-trace determination of mercury by platinum-coated tungsten coil trapping cold vapour atomic absorption spectrometry”, International Journal of Environmental Analytical Chemistry, 1-13, 2022.
• de Wuilloud, J. C., Wuilloud, R. G., Silva, M. F., Olsina, R. A. and Martinez, L. D., “Sensitive determination of mercury in tap water by cloud point extraction pre-concentration and flow injection-cold vapor-inductively coupled plasma optical emission spectrometry”, Spectrochimica Acta Part B: Atomic Spectroscopy, 57, 365-374, 2002.
• Wu, L., Long, Z., Liu, L., Zhou, Q., Lee, Y. I. and Zheng, C., “Microwave-enhanced cold vapor generation for speciation analysis of mercury by atomic fluorescence spectrometry”, Talanta, 94, 146-151, 2012.
• Lee, S. H. and Suh, J. K., “Determination of mercury in tuna fish tissue using isotope dilution-inductively coupled plasma mass spectrometry”, Microchemical Journal, 80(2), 233-236, 2005.
• Voegborlo, R. B. and Akagi, H., “Determination of mercury in fish by cold vapour atomic absorption spectrometry using an automatic mercury analyzer”, Food Chemistry, 100(2), 853-858, 2007.
• Atasoy, M., Yildiz, D., Kula, İ. and Vaizoğullar, A. İ., “Determination and speciation of methyl mercury and total mercury in fish tissue samples by gold-coated W-coil atom trap cold vapor atomic absorption spectrometry”, Food Chemistry, 401, 134152, 2023.
• Altundağ, H., Küpeli, T., Altıntığ, E., Vaizoğullar, A. İ., Kula, I., Tüzen, M. and İmamoglu, M., “Mercury determination in muscle tissue of fish samples using microwave digestion and CVAAS analysis”, Atomic Spectroscopy, 38(1), 32-36, 2017.
• Astolfi, M. L., Protano, C., Marconi, E., Piamonti, D., Massimi, L., Brunori, M., Vitali, M. and Canepari, S., “Simple and rapid method for the determination of mercury in human hair by cold vapour generation atomic fluorescence spectrometry”, Microchemical Journal, 150, 104186, 2019.
• Serafimovski, I., Karadjova, I., Stafilov, T. and Cvetković, J., “Determination of inorganic and methylmercury in fish by cold vapor atomic absorption spectrometry and inductively coupled plasma atomic emission spectrometry”, Microchemical Journal, 89, 42-47, 2008.
• Zhu, S., Chen, B., He, M., Huang, T. and Hu, B., “Speciation of mercury in water and fish samples by HPLC-ICP-MS after magnetic solid phase extraction”, Talanta, 171, 213-219, 2017.
• Angyus, S. B., Darvasi, E., Ponta, M., Petreus, D., Etz, R., Senila, M., Frentiu, M. and Frentiu, T., “Interference-free, green microanalytical method for total mercury and methylmercury determination in biological and environmental samples using small-sized electrothermal vaporization capacitively coupled plasma microtorch optical emission spectrometry”, Talanta, 217, 121067, 2020.
• Bendl, R. F., Madden, J. T., Regan, A. L. and Fitzgerald, N., “Mercury determination by cold vapor atomic absorption spectrometry utilizing UV photoreduction”, Talanta, 68, 1366-1370, 2006.
• Su, Y., Xu, K., Gao, Y. and Hou, X., “Determination of trace mercury in geological samples by direct slurry sampling cold vapor generation atomic absorption spectrometry”, Microchimica Acta, 160, 191-195, 2008.
• Chen, P., Deng, Y., Guo, K., Jiang, X., Zheng, C. and Hou, X., “Flow injection hydride generation for on-atomizer trapping: Highly sensitive determination of cadmium by tungsten coil atomic absorption spectrometry”, Microchemical Journal, 112, 7-12, 2014.
• Matusiewicz, H. and Sturgeon, R. E., “Atomic spectrometric detection of hydride forming elements following in situ trapping within a graphite furnace”, Spectrochimica Acta Part B: Atomic Spectroscopy, 51, 377-397, 1996.
• Docekal, B. and Marek, P., “Investigation of in situ trapping of selenium and arsenic hydrides within a tungsten tube atomizer”, Journal of Analytical Atomic Spectrometry, 16, 831-837, 2001.
• Alp, O. and Ertaş, N., “In-situ trapping arsenic hydride on tungsten coil and comparing interference effect of some hydride forming elements using different types of atomizers”, Microchemical Journal, 128, 108-112, 2016.
• Kula, I., Arslan, Y., Bakırdere, S. and Ataman, O. Y., “A novel analytical system involving hydride generation and gold-coated W-coil trapping atomic absorption spectrometry for selenium determination at ng l− 1 level”, Spectrochimica Acta Part B: Atomic Spectroscopy, 63(8), 856-860, 2008.
• Atasoy, M. and Yildiz, D., “Novel Palladium Coated Tungsten Coil Atom Trap for Ultra-Trace Determination of Selenium by Hydride Generation Atomic Absorption Spectrometry (HGAAS)”, Analytical Letters, 1-15, 2023.
• Atasoy, M., “Development of a new sensitive method for lead determination by platinum-coated tungsten-coil hydride generation atomic absorption spectrometry”, ACS Omega, 8(25), 22866-22875, 2023.
• Choe, K. Y. and Gajek, R., “Determination of trace elements in human urine by ICP-MS using sodium chloride as a matrix-matching component in calibration”, Analytical methods, 8(37), 6754-6763, 2016.
• Guerrero, M. L., Alonso, E. V., Pavón, J. C., Cordero, M. S., and De Torres, A. G., “Simultaneous determination of chemical vapour generation forming elements (As, Bi, Sb, Se, Sn, Cd, Pt, Pd, Hg) and non-chemical vapour forming elements (Cu, Cr, Mn, Zn, Co) by ICP-OES”, Journal of Analytical Atomic Spectrometry, 31(4), 975-984, 2016.
• da Silva, D. L. F., da Costa, M. A. P., Silva, L. O. B., and Dos Santos, W. N. L., “Simultaneous determination of mercury and selenium in fish by CVG AFS”, Food Chemistry, 273, 24-30, 2019.
• Zavvar Mousavi, H., Asghari, A., and Shirkhanloo, H., “Determination of Hg in water and wastewater samples by CV-AAS following on-line preconcentration with silver trap”, Journal of Analytical Chemistry, 65, 935-939, 2010.
• Alp, O., and Ertaş, N., “Determination of inorganic and total mercury by flow injection vapor generation atomic absorption spectrometry using a W-coil atomizer”, Journal of Analytical Atomic Spectrometry, 24(1), 93-96, 2009.
• Peng, M., Li, Z. A., Hou, X., and Zheng, C., “In-atomizer atom trapping on gold nanoparticles for sensitive determination of mercury by flow injection cold vapor generation atomic absorption spectrometry”, Journal of Analytical Atomic Spectrometry, 29(2), 367-373, 2014.