In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium

Year 2022, , 80 - 88, 04.04.2022

Muhammet Dogan

https://doi.org/10.28978/nesciences.1098681

Abstract

Pogostemon erectus (Dalzell) Kuntze is a valuable aquatic-ornamental plant. This study was designed for the multiplication of P. erectus in a liquid culture medium. In this context, shoot tip explants of P. erectus were placed in Murashige and Skoog (MS) food solutions including Zeatin (ZEA: 0.1-2.4 mg/L) and indole acetic acid (IAA: 1.2 mg/L). In addition, experiments were set up in hormone-free environments as controls. In general, high-frequency results were recorded. 100 % regeneration was determined in the treatment of 0.6 mg/L ZEA + 1.2 mg/L IAA. The best results for shoot count and shoot length were obtained with 0.6 mg/L ZEA + 1.2 mg/L IAA and 0.3 mg/L ZEA + 1.2 mg/L IAA, respectively. Generally, low regeneration values were seen in nutrient solutions using high or low doses of ZEA. Shoots were rooted in MS nutrient media with 0.25 mg/L IAA. Then they were transferred to an aquarium and successfully adapted to external conditions.

Keywords

MS medium, Shoot regeneration, Shoot tip explant, Tissue culture

References

• Abdalla, I. B., Ali, M. A. A. A., Ali, O. A., & Elhassan, A. A. (2021). Effect of cytokinins and auxins on micropropagation of shoot tip and nodal explants of two cultivars of sweet potato (Ipomoea batatas (L) Lam). Gezira Journal of Agricultural Science, 1(2), 84-93.
• Ahmad, A., Anis, M., Khanam, M. N., & Alatar, A. A. (2020). Direct shoot organogenesis from shoot tip explants of a highly medicinal valued tree Pterocarpus marsupium Roxb. In Vitro Cellular & Developmental Biology-Plant, 56(5), 670-681.
• Arigundam, U., Variyath, A. M., Siow, Y. L., Marshall, D., & Debnath, S. C. (2020). Liquid culture for efficient in vitro propagation of adventitious shoots in wild Vaccinium vitis-idaea ssp. minus (lingonberry) using temporary immersion and stationary bioreactors. Scientia Horticulturae, 264, 109199.
• Borpuzari, P. P., & Bisht, N. S. (2018). Enhanced rhizome induction and fast regeneration protocol in liquid culture of Dendrocalamus longispathus Kurz: A single step culture. Tropical Plant Research, 6(1), 18-23.
• Demétrio, C. A., de Oliveira Jacob, J. F., Ambrosano, G. B., de Oliveira, Ê. T., & Rodrigues, P. H. V. (2021). In vitro propagation of cambuci (Campomanesia phaea): An endangered exotic fruit and ornamental plant from Brazilian Atlantic Forest. Plant Cell, Tissue and Organ Culture (PCTOC), 145(1), 203-208.
• Dogan, M. (2018). Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Acta Biologica Turcica, 32(2), 78-83.
• Dogan, M. (2019a). In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Anatolian Journal of Botany, 3(1), 1-6.
• Dogan, M. (2019b). Multiple shoot regeneration via indirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. Journal of Animal and Plant Sciences, 29(2), 568-577.
• Doğan, M. (2017). In vitro koşullarda çoğaltılan bazı su bitkilerinin fitoremediasyon potansiyellerinin araştırılması. Karamanoğlu Mehmetbey Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, Karaman.
• Espinosa-Leal, C. A., Puente-Garza, C. A., & García-Lara, S. (2018). In vitro plant tissue culture: means for production of biological active compounds. Planta, 248(1), 1-18.
• Hussain, A., Qarshi, I. A., Nazir, H., & Ullah, I. (2012). Plant tissue culture: current status and opportunities. Recent Advances in Plant In Vitro Culture, IntechOpen, Croatia.
• Kose, M. S. H., Dogan, M., & Sadi, G. (2021). Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze. Biologia, 76(3), 1053-1061.
• Kulus, D. (2020). Influence of growth regulators on the development, quality, and physiological state of in vitro-propagated Lamprocapnos spectabilis (L.) Fukuhara. In Vitro Cellular & Developmental Biology-Plant, 56(4), 447-457.
• Loyola-Vargas V.M., & Avilez-Montalvo R.N. (2018). Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9. In: Loyola-Vargas V., Ochoa-Alejo N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY
• Moola, A. K., & Kumari, B. R. (2019). Direct regeneration of plantlets from shoot tip explants of a vulnerable medicinal plant–Celastrus paniculatus Willd. Journal of Applied Horticulture, 21(3), 189-194.
• Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
• Nowakowska, K., Pacholczak, A., & Tepper, W. (2019). The effect of selected growth regulators and culture media on regeneration of Daphne mezereum L.‘Alba’. Rendiconti Lincei. Scienze Fisiche e Naturali, 30(1), 197-205.
• Oseni, O. M., Pande, V., & Nailwal, T. K. (2018). A review on plant tissue culture, a technique for propagation and conservation of endangered plant species. International Journal of Current Microbiology and Applied Sciences, 7(7), 3778-3786.
• Plessis, H. J. D., Nikolova, R. V., Egan, B. A., & Kleynhans, R. (2021). Preliminary study on in vitro shoot culture of Hibiscus coddii subsp. barnardii, an indigenous South African flowering plant. Ornamental Horticulture, 27, 408-416.
• Rittirat, S., Thammasiri, K., & Klaocheed, S. (2021). In vitro rapid multiplication of a highly valuable ornamental aquatic plant Anubias heterophylla. Trends in Sciences, 18(19)-3, 1-12.
• Singh, A. (2018). Efficient micropropagation protocol for Jatropha curcas using liquid culture medium. Journal of Crop Science and Biotechnology, 21(1), 89-94. Tyub, S., Dar, S. A., Lone, I. M., Mir, A. H., & Kamili, A. N. (2021). A robust in-vitro protocol for shoot multiplication of Echinacea angustifolia. Current Plant Biology, 28, 100221.
• Varutharaju, K., Thilip, C., Raja, P., Thiagu, G., Aslam, A., & Shajahan, A. (2021). An improved liquid culture system for efficient shoot multiplication in Aerva lanata (L.) Juss. Ex Schult. Plant Tissue Culture and Biotechnology, 31(1), 35-42.
• Viella G, K. R. (2021). Resurgence of multiple shoots from photosynthetic explant of red listed Lindernia antipoda (L.) Alston. International Journal of Aquatic Science, 12(3), 105-111.
• Wada, E., & Feyissa, T. (2021). In vitro propagation of two tannia (Xanthosoma sagittifolium (L.) Schott) cultivars from shoot tip explants. Plant Tissue Culture and Biotechnology, 31(1), 25-34.
• Xhulaj, D. B., & Doriana, B. (2019). Effect of plant growth regulators on in vitro plant regeneration of wheat (Triticum aestivum L.) from embryo explants. Journal of Animal and Plant Sciences, 29, 1616-1621.
• Yaseen, M., Ahmad, T., Sablok, G., Standardi, A., & Hafiz, I.A. (2013). Role of carbon sources for in vitro plant growth and development. Molecular Biology Reports, 40(4), 2837-2849.