Comparative analysis of the different regions of skin tissue in Nannospalax xanthodon

Year 2020, Volume: 5 Issue: 2, 92 - 100, 23.06.2020

Burcu Biterge Süt , Ayşe İkinci Keleş Teoman Kankılıç

https://doi.org/10.28978/nesciences.756748

Cited By: 1

Abstract

Blind mole rats (Nannospalax xanthodon Palmer, 1903) are subterranean mammals that are well-known for their high tolerance to hypoxia and resistance to cancer. Due to their unusual habitat, these animals have developed several adaptations during their evolution. Therefore, this study aimed to identify possible structural differences between different regions of the skin in Nannospalax as well as to characterize the histological organization of a specialized skin patch namely scrub sensory organ that have possibly arisen as a result of adaptation to underground life. Skin from the neck, nasal pad and the scrub sensory organ was harvested from wildtype blind mole rats and fixed in 10% formaldehyde. Tissues were embedded into paraffin and blocked via routine histological procedures. 5-micron sections were taken and stained with Hematoxylin & Eosin and Cresyl Violet. Histopathological analysis of the skin revealed that the nasal pad and the scrub sensory organ were significantly thicker compared to the neck skin. Skin epithelia was structurally normal, although it was thicker in the neck skin in comparison to others. We observed structures that are similar to pyramidal cells in the scrub sensory organ. In conclusion, we defined some histological properties of the scrub sensory organ in Nannospalax xanthodon for the first time in the literature. Specifically, the detection of structures that are similar to pyramidal cells is significant. These results indicate structural differences between different skin regions and suggest a role for the scrub sensory organ in somatosensation.

Keywords

Blind mole rat, skin histopathology, somatosensation, pyramidal cell

References

• Adeli, S., Zahmatkesh, M., Ansari Dezfouli M. (2019). Simvastatin Attenuates Hippocampal MMP-9 Expression in the Streptozotocin-Induced Cognitive Impairment. Iran Biomed J. 23(4):262-71.
• Begall S., Burda H., Schleich C.E. (Eds.) (2007). Subterranean Rodents: News from Underground. Springer, Berlin, Heidelberg.
• Bekkers, J. M. (2011). Pyramidal neurons. Curr Biol. 21(24):R975.
• Bronchti, G., Heil, P., Sadka, R., Hess, A., Scheich, H., Wollberg, Z. (2002). Auditory activation of "visual" cortical areas in the blind mole rat (Spalax ehrenbergi). Eur J Neurosci. 16: 311-29.
• Burda, H. (2006). Ear and eye in subterranean mole-rats, Fukomys anselli (Bathyergidae) and Spalax ehrenbergi (Spalacidae): progressive specialization or regressive degeneration? Anim.Biol. 56: 475‒486.
• Catania, K.C. (1999). A nose that looks like a hand and acts like an eye: the unusual mechanosensory system of the star-nosed mole. Journal of Comparative Physiology A: Sensory, Neural, and Behavioral Physiology 185:367–372.
• Crish, S. D., Rice, F. L., Park, T. J., Comer, C. M. (2003). Somatosensory organization and behavior in naked mole-rats I: Vibrissa-like body hairs comprise a sensory array that mediates orientation to tactile stimuli. Brain, Behavior and Evolution, 62(3): 141-151.
• Ebner, F. F. and Kaas, J. H. (2015) Somatosensory System. In The Rat Nervous System. 4th edition, Academic Press, Elsevier.
• Hill, W.C.O., Porter, A., Bloom, R.T., Seago, J., Southwick, M.D. (2009). Field and laboratory studies on the naked mole rat, heterocephalus glaber. Proceedings of the Zoological Society of London 128:455–514.
• İkinci, A., Odacı, E., Baş, O. (2013). Effects of ethyl pyruvate administration on female rats’ pyramidal cells of cornu ammonis after brain ischemia: a stereological and histopathological study. Turk J Med Sci. 43: 354-361.
• Kaplan, S., Onger, M. E., Altunkaynak, B. Z., Elibol, E., Deniz, O. G., Karayiğit, M. Ö., Yarım, M., Marangoz, C., Ragbetli, M. Ç. (2018). Effects of spermine and the passive avoidance learning (PAL) following cerebral ischemia in chicks: Association with neuroprotection of pyramidal cells. J Chem Neuroanat. 88: 41-45”.
• Kazmierczak, P., Müller, U. (2012). Sensing sound: molecules that orchestrate mechanotransduction by hair cells. Trends Neurosci. 35: 220-9.
• Lacey, E.A., Patton, J.L., Cameron, G.N. (2000). Life Underground: The Biology of Subterranean Rodents. Chicago:University of Chicago Press.
• Low, Z. W. K., Li, Z., Owh, C., Chee, P. L., Ye, E., Kai, D., Yang, D. P., Loh, X. J. (2019). Using Artificial Skin Devices as Skin Replacements: Insights into Superficial Treatment. Small. 15(9):e1805453.
• Manns, I. D., Sakmann, B., Brecht, M. (2004). Sub- and suprathreshold receptive field properties of pyramidal neurones in layers 5A and 5B of rat somatosensory barrel cortex. J Physiol. 15;556(Pt 2):601-22.
• Nevo, E., Filippucci, M. G., Redi, C., Korol, A., Beiles, A. (1994). Chromosomal speciation and adaptive radiation of mole rats in Asia Minor correlated with increased ecological stress. Proc Natl Acad Sci U S A. 91; 8160-8164.
• Nevo, E., Filippucci, M. G., Redi, C., Simson, S., Heth, G., Beiles, A. (1995). Karyotype and genetic evolution in speciation of subterranean mole rats of the genus Spalax in Turkey. Biological Journal of the Linnean Society, 54; 203-229.
• Nevo, E. (2013). Stress, adaptation, and speciation in the evolution of the blind mole rat, Spalax, in Israel. Mol. Phylogenet.Evol. 66: 515–525.
• Partha, R., Chauhan, B.K., Ferreira, Z., Robinson, J.D., Lathrop, K., Nischal, K.K., Chikina, M., Clark, N.L. (2017). Subterranean mammals show convergent regression in ocular genes and enhancers, along with adaptation to tunneling. Elife. 6:e25884.
• Petreanu, L., Gutnisky, D. A., Huber, D., Xu, N. L., O'Connor, D. H., Tian, L., Looger, L., Svoboda, K. (2012). Activity in motor-sensory projections reveals distributed coding in somatosensation. Nature. 13;489(7415):299-303.
• Sanyal, S., Jansen, H. G. J., de Grip, W., Nevo, E., de Jong, W. (1990). The eye of the blind mole rat, Spalax ehrenbergi. Invest. Ophthalmol. Vis. Sci. 31: 1398–1404. Spruston, N. (2008). Pyramidal neurons: dendritic structure and synaptic integration. Nat Rev Neurosci. 9(3):206-21.