The effects of EDTA on zinc accumulation in tissues of Cyprinus carpio (Linnaeus 1758)

Year 2021, Volume: 6 Issue: 1, 30 - 38, 25.01.2021

Fahri Karayakar Fikret Seçen Bedii Cicik

https://doi.org/10.28978/nesciences.868065

Abstract

The present study aimed to determine the zinc accumulation levels in the gill, liver, and muscle tissues of Cyprinus carpio (Linnaeus, 1758) under the effect of zinc (2.0 and 4.0 ppm) and zinc + EDTA (2.0 + 4.0 and 4.0 + 8.0 ppm) for 7, 15, and 30 days and the effects of EDTA on zinc accumulation in tissues. Tissue zinc levels were determined by inductively coupled plasma-mass spectrometry (ICP˗MS). No mortality occurred in fish under the exposure times and concentrations with solutions containing zinc only and zinc combined with EDTA. At the beginning of the metal exposure, various behavioral changes were observed in the fish, and those behaviors returned to normal with longer exposure time. The exposure to both zinc alone and zinc combined with EDTA significantly increased metal accumulation in the tissues and organs (P < 0.05) as compared to the control, and a gill > liver > muscle accumulation relationship was found among the tissues. The results obtained showed that EDTA reduced zinc accumulation in the gill, liver, and muscle tissues when compared to the effect of zinc only.

Keywords

Cyprinus carpio, zinc, EDTA, tissue, bioaccumulation

References

• Abdel-Tawwab, M., Mousaad, M.N.M., Sharafeldin, K.M. & Ismaiel, N.E.M. (2013). Changes in Growth and Biochemical Status of Common Carp, Cyprinus carpio L. Exposed to Water-Born Zinc Toxicity for Different Periods. International Aquatic Research, 5(11), 1-9. doi:10.1186/2008-6970-5-11
• Abdullah, S., Javed, M. & Javid, A. (2007). Studies on Acute Toxicity of Metals to the Fish (Labeo rohita). International Journal of Agriculture & Biology, 9(2), 333-337.
• Al-Yousuf, M.H., El-Shahawi, M.S. & Al-Ghais, S.M. (2000). Trace metals in liver, skin and muscle of Lethrinus lentjan fish species in relation to body length and sex. The Science of the Total Environment, 256, 87-94. doi: 10.1016/s0048-9697(99)00363-0
• Benedict, O.O. & Ayotunde, E.O. (2008). Toxicity of Lead to Freshwater Invertebrates, (Water Fleas; Daphnia magna and Cyclop sp.) in Fish Ponds in a Tropical Floodplain. Water Air Soil & Pollution, 192, 39-46. doi:10.1007/s11270-008-9632-0
• Bervoets, L. & Blust, R. (2003). Metal Concentrations in Water, Sediment and Gudgeon (Gobio gobio) from a Pollution Gradient: Relationship with Fish Condition Factor. Environmental Pollution, 126, 9-19. doi:10.1016/s0269-7491(03)00173-8
• Chavan, V.R. & Muley, D.V. (2014). Effect of Heavy Metals on Liver and Gill of Fish Cirrhinus mrigala. International Journal of Current Microbiology and Applied Sciences, 3(5), 277-288.
• De Boeck, G., Meeus, W., Coen, W.D. & Blust, R. (2004). Tissue-Spesific Cu Bioaccumulation Patterns and Differences in Sensitivity to Waterborne Cu in Three Freshwater Fish: Rainbow Trout (Oncorhyncus mykiss), Common Carp (Cyprinus carpio), and Gibel Carp (Carassius auratus gibelio). Aquatic Toxicology, 70, 179-188. doi:10.1016/j.aquatox.2004.07.001
• DeConto Cinier, C., Petit-Ramel, M., Faure, R., Garin, D. & Bouvet, Y. (1999). Kinetics of Cadmium Accumulation and Elimination in Carp Cyprinus carpio Tissues. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 122, 345-352. doi:10.1016/S0742-8413(98)10132-9
• Ebrahimi, M. & Taherianfard, M. (2011). The Effects of Heavy Metals Exposure on Reproductive Systems of Cyprinid Fish from Kor River. Iranian Journal of Fisheries Sciences, 10(1), 13-24.
• Eisler, R. (2000). Handbook of Chemical Risk Assessment Health Hazards to Humans, Plants and Animals. 1 st ed. Boca Raton, London, New York, D.C: Lewis Publishers. Heath, A.G. (1995). Water Pollution and Fish Physiology. 2nd ed. New York, NY, USA: CRC Press.
• Hogstrand, C. (2012). Zinc, Homeostasis and Toxicology of Essential Metals. In: Wood, C.M., Farrell, A.P. & Brauner, C.J. (editor). Homeostasis and Toxicology of Essential Metals. 1 st ed. San Diego, CA, USA: Elsevier, pp. 135-200.
• Lu, N., Sun, S., Song, W. & Jia, R. (2017). Behavioural Toxicity in Zebrafish (Danio rerio) Exposed to Waterborne Zinc and Chromium (VI). Chemistry and Ecology, 33(8), 725-738. doi:10.1080/02757540.2017.1373767
• Morgan, J.D., Sakamoto, T., Grau, E.G. & Iwama, G.K. (1997). Physiological and Respiratory Responses of the Mozambique Tilapia (Oreochromis mossambicus) to Salinity Acclimation. Comparative Biochemistry and Physiology - A Physiology, 117(3), 391-398. doi:10.1016/S0300-9629(96)00261-7
• Muramoto, S. (1980). Effects of Complexans (EDTA, NTA and DTPA) on the Exposure to High Concentrations of Cadmium, Copper, Zinc and Lead. Bulletin of Environmental Contamination and Toxicology, 25, 941-946. doi: 10.1007/BF01985635
• Nicula, M., Gergen, I., Harmanescu, M., Banatean-Dunea, I., Marcu, A., et al. (2011). Assessing the Impact of EDTA Chelating Effect on Some Macro and Microminerals in Prussian Carp (Carassius gibelio) Tissues. Scientific Papers Animal Science and Biotechnologies, 44 (2), 40-44.
• Nowack, B. (1996). Behavior of EDTA in Groundwater a Study of the Surface Reactions of Metal ˗ EDTA Complexes. PhD, Swiss Federal Institute of Technology, Zürich, Switzerland.
• Palaniappan, P.L.R.M., Nishanth, T. & Renju, V.B. (2010). Bioconcentration of Zinc and its Eeffect on the Biochemical Constituents of the Gill Tissues of Labeo rohita: An FT˗IR Study. Infrared Physics & Technology, 53, 103-111. doi:10.1016/j.infrared.2009.10.003
• Palaniappan, R. & Muthulingam, M. (2016). Impact of Heavy Metal, Chromium on Protein Metabolism in Brain and Muscle of Freshwater Fish, Channa striatus (Bloch). International Journal of Current Microbiology and Applied Sciences, 5(7), 638-647. doi:10.20546/ijcmas.2016.507.072
• Preez, H.H., Rensburg, E. & Vuren, J.H.J. (1993). Preliminary Laboratory Investigation of the Bioconcentration of Zinc and Iron in Selected Tissues of the Banded Tilapia, Tilapia sparmanii (Cichlidae). Bulletin of Environmental Contamination and Toxicology, 50, 674-681. doi: 10.1007/BF00194661
• Shukla, V., Dhankhar, M., Prakash, J. & Sastry, K.V. (2007). Bioaccumulation of Zn, Cu and Cd in Channa punctatus. Journal of Environmental Biology, 28(2), 395-397.
• Tunçsoy, M. & Erdem, C. (2014). Accumulation of Copper, Zinc and Cadmium in Liver, Gill and Muscle Tissues of Oreochromis niloticus Exposed to These Metals. Fresenius Environmental Bulletin, 23(5), 1143-1149.
• Van Dyk, J.C., Pieterse, G.M. & Van Vuren, J.H.J. (2007). Histological Changes in the Liver of Oreochromis mossambicus (Cichlidae) After Exposure to Cadmium and Zinc. Ecotoxicology and Environmental Safety, 66, 432-440. doi:10.1016/j.ecoenv.2005.10.012
• Yesilbudak, B. (2009). Zinc and Cadmium Accumulation in Gill, Liver, Kidney and Muscle Tissues of Cyprinus carpio and Oreochromis niloticus. MSc, Cukurova University, Adana, Turkey.