Extraction and Characterization of Chitin and Chitosan from Invasive Alien Swimming Crab Charybdis longicollis

Year 2021, Volume: 6 Issue: 2, 96 - 101, 12.07.2021

Servet Ahmet Doğdu Cemal Turan Tolga Depçi

https://doi.org/10.28978/nesciences.970546

Cited By: 1

Abstract

Chitosan is a biocompatible, biodegradable, non-toxic, antibacterial, antioxidant, and antifungal natural polymer. In this study, chitin and chitosan were chemically isolated from the exoskeleton of invasive swimming crab Charybdis longicollis. In order to obtain the chitin, demineralization, deproteinization, and decolourization steps were applied to the samples. Chitosan was prepared from the isolated chitin by deacetylation at high temperatures. The chemical composition of chitin from C. longicollis was characterized by XRD and FTIR analysis. The yield of chitin extraction from dry crab shells was 25.78 %. The yield of chitosan produced from extracted chitin was 80.23 %. The experimental analyses revealed that the obtained chitin and chitosan could be used as biomaterial.

Keywords

Charybdis longicollis, swimming crab, chitin, chitosan, scaffold, biomaterial

References

• Ahyat, N. M., Mohamad, F., Ahmad, A., & Azmi, A. A. (2017). Chitin and chitosan extraction from Portunus pelagicus. Malaysian Journal of Analytical Sciences, 21(4), 770-777.
• Demir, D., Bölgen, N., Öfkeli, F., & Ceylan, S. (2016). Extraction and characterization of chitin and chitosan from blue crab and synthesis of chitosan cryogel scaffolds. Journal of the Turkish Chemical Society Section A: Chemistry, 3(3), 131-144.
• Deval, M. C. (2020). Population structure and dynamics of the invasive crab Charybdis longicollis parasitised by the rhizocephalan Heterosaccus dollfusi along the Mediterranean coast of Turkey. Biologia, 1-11.
• Holthuis, L. B. (1961). Report on a collection of Crustacea Decapoda and Stomatopoda from Turkey and the Balkans. Zoologische Verhandelingen, 47(1), 1-67.
• Hudson, S. M., & Smith, C. (1998). Polysaccharides: chitin and chitosan: chemistry and technology of their use as structural materials. In Biopolymers from renewable resources (pp. 96-118). Springer, Berlin, Heidelberg.
• Innocenti, G., Pinter, N., & Galil, B. S. (2003). Observations on the agonistic behaviour of the swimming crab Charybdis longicollis Leene infected by the rhizocephalan barnacle Heterosaccus dollfusi Boschma. Canadian Journal of Zoology, 81(1), 173-176.
• Innocenti, G., & Galil, B. S. (2011). Live and let live: invasive host, Charybdis longicollis (Decapoda: Brachyura: Portunidae), and invasive parasite, Heterosaccus dollfusi (Cirripedia: Rhizocephala: Sacculinidae). In the Wrong Place-Alien Marine Crustaceans: Distribution, Biology and Impacts (pp. 583-605). Springer, Dordrecht.
• Kaya, M., Dudakli, F., Asan-Ozusaglam, M., Cakmak, Y. S., Baran, T., Mentes, A., & Erdogan, S. (2016). Porous and nanofiber α-chitosan obtained from blue crab (Callinectes sapidus) tested for antimicrobial and antioxidant activities. LWT-Food Science and Technology, 65, 1109-1117.
• Oduor-Odeto, P. M., Struszezyk, M. H., & Peter, M. G. (2005). Characterisation of chitosan from blowfly larvae and some Crustacean species from Kenyan marine waters prepared under different conditions. Western Indian Ocean Journal of Marine Science, 4(1), 99-108.
• Pillai, C. K., Paul, W., & Sharma, C. P. (2009). Chitin and chitosan polymers: Chemistry, solubility and fiber formation. Progress in Polymer Science, 34(7), 641-678.
• Santos, V. P., Marques, N. S., Maia, P. C., Lima, M. A. B. D., Franco, L. D. O., & Campos-Takaki, G. M. D. (2020). Seafood waste as attractive source of chitin and chitosan production and their applications. International Journal of Molecular Sciences, 21(12), 4290.
• Sikorski, P., Hori, R., & Wada, M. (2009). Revisit of α-chitin crystal structure using high-resolution X-ray diffraction data. Biomacromolecules, 10(5), 1100-1105.
• Stasolla, G., Innocenti, G., & Galil, B. S. (2015). On the diet of the invasive crab Charybdis longicollis Leene, 1938 (Brachyura: Portunidae) in the eastern Mediterranean Sea. Israel Journal of Ecology and Evolution, 61(3-4), 130-134.
• Varun, T. K., Senani, S., Kumar, N., Gautam, M., Gupta, R., & Gupta, M. (2017). Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste. Indian Journal of Animal Research, 51(6).