SYNTHESIS AND ANIMICROBIAL ACTIVITY OF WATER-SOLUBLE SULFONATO CALIX[8]AREN BINDING WITH DNA

Year 2019, Volume: 8 Issue: 1, 615 - 620, 28.01.2019

Mevlüt Bayrakcı , Bahar Yılmaz

https://doi.org/10.28948/ngumuh.517209

Cited By: 2

Abstract

   In this study; it was synthesized a water
soluble calixarene derivative so-called as versatile molecules for the design
and synthesis of receptors having mimic properties or affect biological
functions. The structure of the synthesized sulfonato calix[8]arene (sCLX8) derivative
was clarified by spectroscopic methods such as FT-IR and ¹H-NMR.
Antibacterial activity of the sulfonate calix[8]arene molecule was investigated
by disk diffusion test on gram-positive and gram-negative bacterial lines such
as B. subtilis and E. coli, respectively. Furthermore,
possible interaction between the double-stranded DNA (dsDNA) and calixarene was
studied by agarose gel electrophoresis and UV-Vis spectrophotometry in order to
see the binding behavior of the functionalized water-soluble calixarene
skeleton toward macromolecules.

Keywords

Sulphonato calix, 8, aren, DNA, Disc diffusion, B. Subtilis, E. Coli

DNA İLE BAĞLANABİLEN SUDA ÇÖZÜNÜR SÜLFONATO KALİKS[8]AREN SENTEZİ VE ANTİMİKROBİYAL AKTİVİTESİ

Abstract

   Bu çalışmada; biyolojik fonksiyonları taklit
edebilen veya etkileyen reseptörlerin tasarımı ve sentezi için çok yönlü
moleküller olarak bilinen kaliksarenlerin suda çözünür bir türevi
sentezlenmiştir. Sentezlenen sülfonato kaliks[8]aren (sCLX8) türevinin yapısı
FT-IR ve ¹H-NMR gibi spektroskopik yöntemlerle aydınlatılmıştır.
Yapısı aydınlatılan sülfonato kaliks[8]aren molekülünün B.subtilis gibi gram pozitif ve E.coli
gibi gram negatif özellikteki bakteri hatları üzerine disk difüzyon testi ile
antibakteriyel aktivitesi incelenmiştir. Yine fonksiyonel hale getirilmiş suda
çözünür kaliksaren iskeletinin, makromoleküllere karşı bağlanma davranışını
görebilmek için çift zincirli DNA (dsDNA) ile olan muhtemel etkileşimi agaroz
jel elektroforezi ve UV-Vis spektrofotometresi ile çalışılmıştır.

Keywords

Sülfonato kaliks, 8, aren, DNA, Disk difüzyon, B. Subtilis, E. Coli

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