Investigation of Lipid Peroxidation and Antioxidant Enzyme Activity in Sleep Apnea Patients

Year 2016, , 36 - 40, 15.08.2016

Hakan Turan Canan Demir

https://doi.org/10.20863/nsd.77315

Cited By: 1

Abstract

Aim:The goal of this study was to investigate the activities of malondialdehyde (MDA), a product of lipid peroxidation, and glutathione peroxidase (GPx), anantioxidant enzyme, in sleep apnea patients.

Method: The study involved 40 healthy control patients and 40 sleep apnea patients.  Participants in the experimental group were between the ages of 18 and 65, were diagnosed and treated in the Respiratory Diseases Clinic of Yüzüncü Yıl University Medical Faculty.  Malondialdehyde (MDA) and glutathione peroxidase (GPx) levelsin blood samples were measured using spectrophotometry.

Findings:  According to our results, the difference in the enzyme activity of glutathione peroxidase (GPx) between the experimental group (0.03 ± 0.04U/ml) and the control group (0.06 ± 0.03 U/ ml) was significant (p<0.001).MDA levels were determined to be3.292 U/L (± 0.724U/L) in the experimental groupand 0.882 U/L (± 0.226) in the control group (p<0.001).

Conclusion:  Based on our results, we can conclude that sleep apnea causes a decrease in the GPx enzyme, by means of the body’s antioxidant defense system,and an increase in malondialdehyde, a symptom of oxidative stress.

 

Keywords

Sleep Apnea, MDA; GPx

Investigation of Lipid Peroxidation and Antioxidant Enzyme Activity in Sleep Apnea Patients

Abstract

References

• Çelik Y, 2015. Obstrüktif Uyku Apne Sendromu Olan Bireylerde Metabolik Sendrom ve Beslenme Durumlarının Değerlendirilmesi (doktora tezi, basılmamış). BÜ, Sağlık Bilimleri Enstitüsü, Ankara.
• Ivanhoe JR, Cibirka RM, Lefebvre CA, Parr GR. Dental consideration in upper airway sleep disorders: A review o the literature. J Prosthet Dent, 1999 82:685-698.
• Christou K, Markoulis N, Moulas AN, Pastaka C, Gourgoulianis KI. Reactive oxygen metabolites (ROMs) as an index of oxidative stress in obstructive sleep apnea patients. Sleep Breath, 2003a 7(3): 105–10.
• Barceló A, Barbé F, de la Peña M, Vila M, Pérez G, et al. Antioxidant status in patients with sleep apnoea and impact of continuous positive airway pressure treatment. Eur Respir J., 2006 27(4): 756–60.
• Christou K, Moulas AN, Pastaka C, Gourgoulianis KI. Antioxidant capacity in obstructive sleep apnea patients. Sleep Med, 2003b 4(3): 225–8.
• Anonim, 2015f. https://tr.wikipedia.org/wiki/Antioksidan. Erişim tarihi: 28.11.2015.
• Benzer, F., Temizer OS. Fasciola hepatica ile Enfekte Koyunlarda Lipid Peroksidasyonu, Antioksidant Enzimler ve Nitrik Oksit Düzeyleri. Turk J Vet Anim Sci., 2003 27 : 657-661.
• Muhsiroğlu O. Beslenme ve Kanser - Hasta Bilgilendirme Kitapcığı. Gata Basımevi. Ankara: Türkiye; 2007.
• Beutler E. Red Cell Metabolism. A manual of biochemical methods. 3th Ed. Grune & Stratton. Orlando, 1984 72-73,74-75,105-106.
• Gutteridge, JM. Lipit peroxidation and antioxidants as biomarkers of tissue damage, Clin. Chem.,1995 41 (12): 1819–1828.
• Del Ben M, Fabiani M, Loffredo L, Polimeni L, Carnevale R, Baratta F, Brunori M, Albanese F, Augelletti T, Violi F, Angelico F. Oxidative stress mediated arterial dysfunction in patients with obstructive sleep apnoea and the effect of continuous positive airway pressure treatment. BMC Pulm Med. 2012;12:36.
• Lavie L, Vishnevsky A, Lavie P. Evidence for Lipid Peroxidation in Obstructive Sleep Apnea. Sleep Disordered Breathing, 2003 27:1
• Masotti L, Casali E, Gesmundo N, Sartor G, Galeotti T, Borrello S, Piretti MV, Pagliuca G. Lipid peroxidation in cancer cells: chemical and physical studies. Ann N Y Acad Sci, 1988 551: 47-57.
• Celec P, Hodosy J, Behuliak M, Pálffy R, Gardlík R, Halčák L, Mucska I. Oxidative and carbonyl stress in patients with obstructive sleep apnea treated with continuous positive airway pressure. Sleep Breath. 2012;16:393–398.
• Ntalapascha M, Makris D, Kyparos A, Tsilioni I, Kostikas K, Gourgoulianis K, Kouretas D, Zakynthinos E. Oxidative stress in patients with obstructive sleep apnea syndrome. Sleep Breath. 2013;17:548–555.
• Kang IG, Jung JH, Kim ST. The effect of obstructive sleep apnea on DNA damage and oxidative stress. Clin Exp Otorhinolaryngol. 2013;6:68–72.
• Carpagnano GE, Kharitonov SA, Resta O, Foschino-Barbaro MP, Gramiccioni E, Barnes PJ. 8-Isoprostane, a marker of oxidative stress, is increased in exhaled breath condensate of patients with obstructive sleep apnea after night and is reduced by continuous positive airway pressure therapy. Chest. 2003;124:1386–1392.
• Christou K, Markoulis N, Moulas AN, Pastaka C, Gourgoulianis KI. Reactive oxygen metabolites (ROMS) as an index of oxidative stress in obstructive sleep apnea patients.Sleep Breath. 2003;7:105–110.
• Wali SO, Bahammam AS, Massaeli H, Pierce GN, Iliskovic N, Singal PK, Kryger MH. Susceptibility of LDL to oxidative stress in obstructive sleep apnea. Sleep.1998;21:290–296.
• Ozturk L, Mansour B, Yuksel M, Yalcin AS, Celicoglu F, Gokhan N. Lipid peroxidation and osmotic fragility of red blood cells in sleep apnea patients. Clin Chim Acta. 2003;332:83–88.
• Christou K, Markoulis N, Moulas AN, Pastaka C, Gourgoulianis KI. Reactive oxygen metabolites (ROMS) as an index of oxidative stress in obstructive sleep apnea patients.Sleep Breath. 2003;7:105–110.
• Jordan W, Reinbacher A, Cohrs S, Grunewald RW, Mayer G, Rüther E, Rodenbeck A. Obstructive sleep apnea: Plasma endothelin-1 precursor but not endothelin-1 levels are elevated and decline with nasal continuous positive airway pressure. Peptides.2005;26:1654–1660.
• Ben MD, Albanese F, Augelletti T, et al. Oxidative stress mediated arterial dysfunction in patients with obstructive sleep apnoea and the effect of continuous positive airway pressure treatment. . BMC Pulmonary Medicine, 2012; 12:36.
• Katsoulis K, Kontakiotis T, Spanogiannis D, Vlachogiannis E, Kougioulis M, Gerou S, Daskalopoulou E. Total antioxidant status in patients with obstructive sleep apnea without comorbidities: the role of the severity of the disease. Sleep Breath, 2011; 15(4):86.
• Lee S.D, Ju G. et al. The association of oxidative stress with central obesity in obstructive sleep apnea. Sleep Breath, 2012; 16:511–517.