Determination of Antibacterial Activity of St. John's Wort (Hypericum perforatum L.) Oil, Nigella Sativa Oil, Clove (Eugenia caryophyllata) Oil, Orange Peel (Citrus sinensis) and Garlic (Allium sativa) Oil Against Microorganisms Isolated From Clinical Samples

Abstract

Objective: The aim of this study is to detect St. John's Wort, Nigella sativa, Clove, Orange Peel and Garlic oil on bacteria isolated from blood culture to determine its antibacterial effect. Methods: One hundered blood samples sent to … Medical Microbiology Laboratory between 1 June 2021 and 1 January 2022 were analyzed with blood culture system. Bacteria isolated from blood culture were passaged into blood agar. Bacterial suspension was prepared from the bacterial colonies at 0.5 Mc Farland turbidity. In order to determine the antibacterial activity of plant extract oils, Minimum Inhibition Concentration and Minimal Bactericidal Concentration values were determined by liquid microdilution method. Also, the zone diameters of the disc diffusion method were measured. Results: The antibacterial effect of plant extract oils was detected on only 10 of the 100 clinical samples included in the study. St. John's Wort oil used in these 10 samples showed the most effective antibacterial effect of 7.81 µg/mL against Staphylococcus haemolyticus and Enterobacter aerogenes. Garlic oil showed the most effective antibacterial effect against Escherichia coli and Staphylococcus haemolyticus at 7.81 µg/mL. Nigella sativa oil showed the most effective antibacterial effect against Staphylococcus haemolyticus at 3.9 µg/mL. Orange Peel oil showed the most effective antibacterial effect against Enterococcus faecalis at 1.95 µg/mL. The minimum inhibition concentration at which the oils were effective on microorganisms was determined by comparing them with standard control strains. Conclusion: More clinical isolates and high-dose studies are needed to determine the effectiveness of plant extract oils. Garlic oil Escherichia coli, Staphylococcus haemolyticus and Enterobacter aerogenes, St. John's wort oil Staphylococcus haemolyticus and Enterobacter aerogenes, Nigella sativa) oil on Staphylococcus haemolyticus has been found to be effective

Keywords

• Antibacterial activity
• Plant extract
• Blood culture
• Microdilution
• Zone diameter
• Antibacterial activity, Plant extract, Blood culture, Microdilution, Zone diameter

References

1. 1. Ferrer R, Martin-Loeches I, Phillips G. Empiric antibiotic treatment reduces mortality in severe sepsis and septic shock from the first hour: results from a guideline-based performance improvement program. Crit Care Med. 2014; 42, 1749-1755. https://doi.org/10.1097/CCM.0000000000000330
2. 2. Dellinger RP, Levy MM, Rhodes A. Surviving sepsis campaign: international guidelines for management of severe sepsis and septic shock: 2012. Crit Care Med. 2013; 41, 580-637. https://doi.org/10.1097/CCM.0b013e31827e83af
3. 3. Levy MM, Artigas A, Phillips GS. Outcomes of the surviving sepsis campaign in intensive care units in the USA and europe: a prospective cohort study. Lancet Infect Dis. 2012; 12, 919-924. https://doi.org/10.1016/S1473-3099(12)70239-6.
4. 4. Marshall JC, Dellinger RP, Levy M. The surviving sepsis campaign: a history and a perspective. Surg Infect. 2010; 11, 275-281. https://doi.org/10.1089/sur.2010.024.
5. 5. Levy M M, Dellinger RP, Townsend SR. The surviving sepsis campaign: results of an international guideline-based performance improvement program targeting severe sepsis. Crit Care Med. 2010; 38, 367-374. https://doi.org/10.1097/CCM.0b013e3181cb0cdc.
6. 6. Huang L, Zhang YY, Sun LY. Time to positivity of blood culture can predict different candida species instead of pathogen concentration in candidemia. Eur J Clin Microbiol Infect Dis. 2013; 32, 917-922. https://doi.org/10.1007/s10096-013-1826-8.
7. 7. Taur Y, Cohen N, Dubnow S, Paskovaty A, Seo SK. Effect of antifungal therapy timing on mortality in cancer patients with candidemia. Antimicrob Agents Chemother. 2010; 54, 184-190. https://doi.org/10.1128/AAC.00945-09.
8. 8. Abdel-Wahhab MA, Aly SE. Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis. Journal of Applied Toxicolgy. 2005; 25 (3): 218–223. https://doi.org/10.1002/jat.1057.

9. 9. Demir HN. Investigation of the Quality of Some Nigella sativa and Grape Seed Oils. Erciyes University Faculty of Pharmacy. Graduation Paper, 2014..
10. 10. Piras A, Rosa A, Morongiu B, Porcedda S, Falconieri D, Dessi MA, Özçelik B, Koca U. Chemical Composition and in vitro Bioactivity of the Volatile and Fixed Oils of Nigella sativa L. Extracted by Supercritical Carbondioxide. Elsevier .2013; 46, 317-323. https://doi.org/10.1016/j.indcrop.2013.02.013.
11. 11. Nannapaneni R, Chalova VI, Crandall PG, Ricke SC, Johnson MG, O’Bryan CA. Campylobacter and Arcobacter species sensitivity to commercial orange oil fractions. Int J Food Microbio.l 2009; 129, 43–49. https://doi.org/10.1016/j.ijfoodmicro.2008.11.008.
12. 12. Magwa ML, Gundidza M, Gweru N. Chemical composition and biological activities of essential oil from the leaves of sesuvium portulacastrum. J Ethnopharmacol. 2006; 103, 85-89. https://doi.org/10.1016/j.jep.2005.07.024.
13. 13. Cortes-Rojas DF, de Souza CR F, Oliveira WP. Clove (Syzygium aromaticum): a precious spice. Asian Pac J Trop Bio. 2014; 4, 90–96. https://doi.org/10.1016/S2221-1691(14)60215-X.
14. 14. Cavallito CJ, Bailey JH and Buck J. The antibacterial principle of Allicin, Allium sativum. III. The precursor and “essential oil” of garlic. J Am Chem Soc. 1944; 66:1950-1951. https://doi.org/10.1021/ja01239a048.
15. 15. Alizadeh Behbahani B, Shahidi F, Yazdi FT, Mortazavi SA, Mohebbi M. Use of Plantago major seed mucilage as a novel edible coating incorporated with Anethum graveolens essential oil on shelf life extension of beef in refrigerated storage. Int J Biol. Macromol.2017;94:515-526. https://doi.org/10.1016/j.ijbiomac.2016.10.055.
16. 16. CLSI. Performance Standards for Antimicrobial Testing; 23rd Informational Supplement. CLSI document M100-S23. Wayne, PA: Clinical and Laboratory Standards Institute.2013.
17. 17. Kuang CL, Lv D, Shen GH, Li SS, Luo QY, Zhang ZQ. Chemical composition and antimicrobial activities of volatile oil extracted from Chrysanthemum morifolium Ramat. J Food Sci Technol. 2018;55(7):2786-2794. https://doi.org/10.1007/s13197-018-3203-1.
18. 18. Rojas J, Ndong Ntoutoume GM, Martin P, Morillo,M. Antibacterial Activity and Reversal of Multidrug Resistance of Tumor Cells by Essential Oils from Fresh Leaves, Flowers, and Stems of Montanoa quadrangularis Schultz Bipontinus (Asteraceae) Collected in Mérida-Venezuela. Biomolecules. 2021; 11(4): 605. https://doi.org/10.3390/biom11040605.
19. 19. Park S, Mun S, Kim YR. Influences of added surfactants on the water solubility and antibacterial activity of rosemary extract. Food science and biotechnology. 2020; 29(10): 1373–1380. https://doi.org/10.1007/s10068-020-00792-w.
20. 20. Lyles JT, Kim A, Nelson K, Bullard-Roberts AL, Hajdari A, Mustafa, B. et al. The Chemical and Antibacterial Evaluation of St. John's Wort Oil Macerates Used in Kosovar Traditional Medicine. Frontiers in microbiology. 2017; 8: 1639. https://doi.org/10.3389/fmicb.2017.01639.
21. 21. Kocoglu E, Kalcioglu MT, Uzun L, Zengin F, Celik S, Serifler S. et al. In Vitro Investigation of the Antibacterial Activity of Nigella sativa Oil on Some of the Most Commonly Isolated Bacteria in Otitis Media and Externa. The Eurasian journal of medicine, 2019; 51(3): 247–251. https://doi.org/10.5152/eurasianjmed.2019.18386.
22. 22. Ginting EV, Retnaningrum E, Widiasih DA. Antibacterial activity of clove (Syzygium aromaticum) and cinnamon (Cinnamomum burmannii) essential oil against extended-spectrum β-lactamase-producing bacteria. Vet World. 2021;14(8):2206-2211. https://doi.org/10.14202/vetworld.2021.2206-2211.
23. 23. Guo C, Shan Y, Yang Z, Zhang L, Ling W, Liang Y, et al. Chemical composition, antioxidant, antibacterial, and tyrosinase inhibition activity of extracts from Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) peel. Journal of the science of food and agriculture. 2020; 100(6): 2664–2674. https://doi.org/10.1002/jsfa.10297.
24. 24. Du J, Bao T, Wang Z, Sun J. A combination of garlic oil and cooked chilli oil could be effective and efficient for pigeon production. J Anim Physiol Anim Nutr.2021;00:1-10. https://doi.org/10.1111/jpn.13646.
25. 25. Leclercq R, Cantón R, Brown DF, Giske CG, Heisig P, MacGowan AP. et al. EUCAST expert rules in antimicrobial susceptibility testing. Clinical microbiology and infection: the official publication of the European Society of Clinical Microbiology and Infectious Diseases.2013; 19(2): 141–160. https://doi.org/10.1111/j.1469-0691.2011.03703.x.
26. 26. Baba E. In vitro antibacterial effect of orange (Citrus sinensis) peel essential oil against bacterial fish pathogens. Journal of Suleyman Demirel University Egirdir Fisheries Faculty.2018; 14(3): 208-214. https://doi.org/10.22392/egirdir.391075.
27. 27. Koptaget E, Özbek A. Investigation of Antimicrobial Efficacy of Cinnamon Oil and Clove Oil by Microbiological Quantification Method, Sakarya University Institute of Health Sciences, Master Thesis, 2019.
28. 28. Emeka L B, Emeka P M, Han T M. Antimicrobial activity of Nigella sativa L. seed oil against multi-drug resistant Staphylococcus aureus isolated from diabetic wounds. Pak J Pharm Sci 2015; 28(6):1985-1990.
29. 29. Kozan G, Semiz G. Chemical Composition of Essential Oils, Comparison of Antibacterial and Antioxidant Effects of Allium Sativum L. (Kastamonu and Denizli Local) Plant, Institute of Science, Master Thesis, 2012.
30. 30. Kim J W, Kim Y.S, Kyung K H. Inhibitory activity of essential oils of garlic and onion against bacteria and yeasts. Journal of Food Protection 2004; 67, 499-504. https://doi.org/10.4315/0362-028X-67.3.499.
31. 31. O’Gara EA, Hill D J, Maslin DJ. Activities of Garlic Oil, Garlic Powder, and Their Diallyl Constituents against Helicobacter pylori. Applied and Environmental Microbiology 2000; 66, 2269-2273. https://doi.org/10.1128/AEM.66.5.2269-2273.2000.
32. 32. Abu-Shanab B, Adwan G, Jarrar N, Abu-Hıjleh A, Adwan K. Antibacterial Activity of Four Plant Extracts Used in Palestine in Folkloric Medicine against Methicillin-Resistant Staphylococcus aureus. Turk J Biol 2006; 30, 195-198.