Role of 6-Shogaol Against Ovarian Torsion Detorsion-Induced Reproductive Organ Damage

Abstract

6-Shogaol was examined on ovarian torsion detorsion (T/D) rat model to find out the potential beneficial results exist or not. 4 groups were composed to establish the experimental step. Sprague-Dawley female rats were used in the experiment. Each group included 8 rats and 32 rats were examined totally. Groups were designed as: Sham, T/D, T/D+Dimethyl sulfoxide (DMSO) and T/D+6-Shogaol groups. Sham group: Only abdominal incision was performed and closed without any additional process. T/D group: 3 hours torsion and 3 hours detorsion process were established following the abdominal incision. T/D+DMSO group: 0.3 ml of DMSO was given as intraperitoneal 30 minutes before detorsion. T/D+6-Shogaol group: 6-Shogaol was administered as intraperitoneal 30 minutes prior to detorsion. At the end of the experiment, high doses of anesthesia was preferred for the euthanasia of the rats and the ovarian tissues were excised in order to obtain samples. Oxidative biomarkers such as MPO activity, OSI, IL-1β, MDA, TNF-α and TOS levels elevated significantly but TAS and SOD levels diminished in T/D group compared to sham group. On the other hand, 6-Shogaol administration provided a decrease in oxidant parameters and elevated TAS and SOD levels, antioxidant mediators. Consequently, 6-Shogaol demonstrated beneficial activity by protecting the ovarian tissues against ovarian injury induced by experimental T/D rat model.

Keywords

• 6-Shogaol,Ovarian Torsion Detorsion,Ovary,Rat

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