Research Article
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Potentiation of Cell Death and DNA Damage Through 5-Fluorouracil and Ferulic Acid Coadministration in p53 Mutant HT-29 Cell Lines

Year 2023, Volume: 4 Issue: 1, 1 - 7, 30.01.2023
https://doi.org/10.56766/ntms.1091833

Abstract

When the Mediterranean diet is set in focus, scientific studies report a strong statistical correlation between human nutrition, diet, and cancer incidence. Considering the anticancer effects of a fiber-rich diet, it is understood that the anticancer effect is not only due to the bulk cellulosic material load but also related to the increased bioavailability of cellulose-bound bioactive (anticancer) compounds released due to intestinal microfloral activities. Ferulic acid (FA) is one of the components found ubiquitously in the fiber fraction of plant food. Because of its effects on cancer cell viability and its association with a low incidence of cancer concerning a high-fiber diet, FA can be considered an anticancer agent. Here in this work, it was investigated whether FA can potentiate the effects of anticancer drugs at lower doses. For this, a general anticancer drug named 5-Fluorouracil (5-FU) was used, and potentiation tests were performed on two cancer cell lines, namely A2780 besides HT-29, which has the homozygous mutation for p53. The results are interpreted as follows: anticancer effect of 5-FU was readily potentiated with 200 µM of FA in both cancer cell lines, and DNA damage-induced with 5-FU was enhanced with co-administration of FA. When cell viability and DNA damage of A2780 and HT-29 lines are evaluated together, we think it is most probable that 5-FU and FA administered jointly show its anticancer effect, especially by strengthening the apoptosis pathway triggered by DNA damage.
If it might be possible to uncover the mechanism that drove DNA damage mediated apoptosis in p53 mutant HT-29 cells in our work we may shed light on the treatment of chemotherapy-resistant cancer incidences.

Supporting Institution

partly by Bartın University Central Research Laboratory, Research and Application Center

Project Number

NONE

References

  • Refrences 1 . Erden Y. Capsanthin Stimulates the Mitochondrial Apoptosis-Mediated Cell Death, following DNA Damage in MCF-7 Cells. Nutr Cancer 2020; 73: 662–670.
  • 2 . Ou S, Kwok K-C. Ferulic acid: pharmaceutical functions, preparation and applications in foods. J Sci Food Agric 2004; 84: 1261–1269.
  • 3 . Isanga J, Zhang GN. Soybean bioactive components and their implications to health - A review. Food Rev Int 2008; 24: 252–276.
  • 4 . K A. AA. Effect of Lepidium sativum on Lipid Profiles and Blood Glucose in Rats Effect of Lepidium sativum on Lipid Profiles and Blood Glucose in Rats. J Physiol Pharmacol Adv 2012; 2: 277–281.
  • 5 . Mishra N, Mohammed A, Rizvi S. Efficacy of Lepidium Sativum to act as an anti-diabetic agent. Prog Heal Sci 2017; 7: 44–53.
  • 6 . Vasanthi, HR, ShriShriMal N, Das DK. Retraction Notice: Phytochemicals from Plants to Combat Cardiovascular Disease. Curr Med Chem 2012; 19: 2242–2251.
  • 7 . Serra F, Spatafora F, Toni S, Farinetti A, Gelmini R, Mattioli AV. Polyphenols, Olive oil and Colonrectal cancer: the effect of Mediterranean Diet in the prevention. Acta Biomed 2021; 92: 1–6.
  • 8 . Djuric Z, Rifkin S. A New Score for Quantifying Adherence to a Cancer-Preventive Mediterranean Diet. Nutr Cancer 2022; 74: 579–591.
  • 9 . Rödel F, Frey B, Multhoff G, Gaipl U. Contribution of the immune system to bystander and non-targeted effects of ionizing radiation. Cancer Lett 2015; 356: 105–113.
  • 10 . Marín A, Martín M, Liñán O, Alvarenga F, López M, Fernández L et al. Bystander effects and radiotherapy. Reports Pract Oncol Radiother 2015; 20: 12–21.
  • 11 . Chen Y, Jungsuwadee P, Vore M, Butterfield DA, St. Clair DK. Collateral damage in cancer chemotherapy: Oxidative stress in nontargeted tissues. Mol Interv 2007; 7: 147–156.
  • 12 . Kumari P, Ghosh B, Biswas S. Nanocarriers for cancer-targeted drug delivery. J Drug Target 2015; 00: 1–13.
  • 13 . Sui M, Liu W, Shen Y. Nuclear drug delivery for cancer chemotherapy. J Control Release 2011; 155: 227–236.
  • 14 . Taper HS, De Gerlache J, Lans M, Roberfroid M. Non‐toxic potentiation of cancer chemotherapy by combined C and K3 vitamin pre‐treatment. Int J Cancer 1987; 40: 575–579.
  • 15 . Casado-Zapico S, Rodriguez-Blanco J, García-Santos G, Martín V, Sánchez-Sánchez AM, Antolín I et al. Synergistic antitumor effect of melatonin with several chemotherapeutic drugs on human Ewing sarcoma cancer cells: Potentiation of the extrinsic apoptotic pathway. J Pineal Res 2010; 48: 72–80.
  • 16 . ERDEN Y, GÜNAY S. TÜMÖR HÜCRELERİ APOPTOZ FAKTÖRÜ (TCApF)’NİN İNSAN PROSTAT VE MEME KANSERİ HÜCRE HATLARI ÜZERİNE SİTOTOKSİK VE GENOTOKSİK ETKİLERİNİN BELİRLENMESİ. İnönü Üniversitesi Sağlık Hizmetleri Mesl Yüksek Okulu Derg 2020; 8: 356–366.
  • 17 . Kang. Implication of necrosis-linked p53 aggregation in acquired apoptotic resistance to 5-FU in MCF-7 multicellular tumour spheroids. Oncol Rep 2010; 24: 4163–8.
  • 18 . Dewanto V, Wu X, Liu RH. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 2002; 50: 4959–4964.
  • 19 . Wilson TA, Nicolosi RJ, Woolfrey B, Kritchevsky D. Rice bran oil and oryzanol reduce plasma lipid and lipoprotein cholesterol concentrations and aortic cholesterol ester accumulation to a greater extent than ferulic acid in hypercholesterolemic hamsters. J Nutr Biochem 2007; 18: 105–112.
  • 20 . Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55–63.
  • 21 . Tekin S, Sandal S, Colak C. Effects of Apelin-13 on Human Prostate Cancer Lines [Insan Prostat Kanseri Hucre Serilerinde Apelin-13’un Etkileri]. Med Sci | Int Med J 2014; 3: 1427.
  • 22 . Genc ZK, Tekin S, Sandal S, Sekerci M, Genc M. Synthesis and DFT studies of structural and some spectral parameters of nickel(II) complex with 2-(2-hydroxybenzoyl)-N-(1-adamantyl) hydrazine carbothioamide. Res Chem Intermed 2015; 41: 4477–4488.
  • 23 . Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184–191.
  • 24 . Erden Y. Sour black mulberry (Morus nigra L.) causes cell death by decreasing mutant p53 expression in HT-29 human colon cancer cells. Food Biosci 2021; 42: 101113.
  • 25 . Hosseini F, Sam MR, Jabbari N, Mozdarani H. Modulating Survivin as a Radioresistant Factor, Caspase-3, and Apoptosis by Omega-3 Docosahexaenoic Acid Sensitizes Mutant-p53 Colorectal Cancer Cells to γ-Irradiation. Cancer Biother Radiopharm 2018; 33: 387–395.
  • 26 . Sritharan S, Sivalingam N. Curcumin induced apoptosis is mediated through oxidative stress in mutated p53 and wild type p53 colon adenocarcinoma cell lines. J Biochem Mol Toxicol 2021; 35: 1–10.
  • 27 . Węglarz L, Molin I, Orchel A, Parfiniewicz B. colon cancer HT-29 cells treated with inositol hexaphosphate. 2006; 53: 349–356.
  • 28 . Yu JJ, Lee KB, Mu C, Li Q, Abernathy T V., Bostick-Bruton F et al. Comparison of two human ovarian carcinoma cell lines (A2780/CP70 and MCAS) that are equally resistant to platinum, but differ at codon 118 of the ERCC1 gene. Int J Oncol 2000; 16: 555–560.
  • 29 . Lee JG, Ahn JH, Kim TJ, Lee JH, Choi JH. Mutant p53 promotes ovarian cancer cell adhesion to mesothelial cells via integrin β4 and Akt signals. Sci Rep 2015; 5: 1–12.
  • 30 . Muthusamy G, Balupillai A, Ramasamy K, Shanmugam M, Gunaseelan S, Mary B et al. Ferulic acid reverses ABCB1-mediated paclitaxel resistance in MDR cell lines. Eur J Pharmacol 2016; 786: 194–203.
  • 31 . Muthusamy G, Gunaseelan S, Prasad NR. Ferulic acid reverses P-glycoprotein-mediated multidrug resistance via inhibition of PI3K/Akt/NF-κB signaling pathway. J Nutr Biochem 2019; 63: 62–71.
  • 32 . Saenglee S, Jogloy S, Patanothai A, Senawong T. Cytotoxic effects of peanut phenolic compounds possessing histone deacetylase inhibitory activity on human colon cancer cell lines. Turkish J Biol 2016; 40: 1258–1271.
  • 33 . Sarwar T, Zafaryab M, Husain MA, Ishqi HM, Rehman SU, Moshahid Alam Rizvi M et al. Redox cycling of endogenous copper by ferulic acid leads to cellular DNA breakage and consequent cell death: A putative cancer chemotherapy mechanism. Toxicol Appl Pharmacol 2015; 289: 251–261.
  • 34 . Gao J, Yu H, Guo W, Kong Y, Gu lina, Li Q et al. The anticancer effects of ferulic acid is associated with induction of cell cycle arrest and autophagy in cervical cancer cells. Cancer Cell Int 2018; 18: 1–9.
Year 2023, Volume: 4 Issue: 1, 1 - 7, 30.01.2023
https://doi.org/10.56766/ntms.1091833

Abstract

Project Number

NONE

References

  • Refrences 1 . Erden Y. Capsanthin Stimulates the Mitochondrial Apoptosis-Mediated Cell Death, following DNA Damage in MCF-7 Cells. Nutr Cancer 2020; 73: 662–670.
  • 2 . Ou S, Kwok K-C. Ferulic acid: pharmaceutical functions, preparation and applications in foods. J Sci Food Agric 2004; 84: 1261–1269.
  • 3 . Isanga J, Zhang GN. Soybean bioactive components and their implications to health - A review. Food Rev Int 2008; 24: 252–276.
  • 4 . K A. AA. Effect of Lepidium sativum on Lipid Profiles and Blood Glucose in Rats Effect of Lepidium sativum on Lipid Profiles and Blood Glucose in Rats. J Physiol Pharmacol Adv 2012; 2: 277–281.
  • 5 . Mishra N, Mohammed A, Rizvi S. Efficacy of Lepidium Sativum to act as an anti-diabetic agent. Prog Heal Sci 2017; 7: 44–53.
  • 6 . Vasanthi, HR, ShriShriMal N, Das DK. Retraction Notice: Phytochemicals from Plants to Combat Cardiovascular Disease. Curr Med Chem 2012; 19: 2242–2251.
  • 7 . Serra F, Spatafora F, Toni S, Farinetti A, Gelmini R, Mattioli AV. Polyphenols, Olive oil and Colonrectal cancer: the effect of Mediterranean Diet in the prevention. Acta Biomed 2021; 92: 1–6.
  • 8 . Djuric Z, Rifkin S. A New Score for Quantifying Adherence to a Cancer-Preventive Mediterranean Diet. Nutr Cancer 2022; 74: 579–591.
  • 9 . Rödel F, Frey B, Multhoff G, Gaipl U. Contribution of the immune system to bystander and non-targeted effects of ionizing radiation. Cancer Lett 2015; 356: 105–113.
  • 10 . Marín A, Martín M, Liñán O, Alvarenga F, López M, Fernández L et al. Bystander effects and radiotherapy. Reports Pract Oncol Radiother 2015; 20: 12–21.
  • 11 . Chen Y, Jungsuwadee P, Vore M, Butterfield DA, St. Clair DK. Collateral damage in cancer chemotherapy: Oxidative stress in nontargeted tissues. Mol Interv 2007; 7: 147–156.
  • 12 . Kumari P, Ghosh B, Biswas S. Nanocarriers for cancer-targeted drug delivery. J Drug Target 2015; 00: 1–13.
  • 13 . Sui M, Liu W, Shen Y. Nuclear drug delivery for cancer chemotherapy. J Control Release 2011; 155: 227–236.
  • 14 . Taper HS, De Gerlache J, Lans M, Roberfroid M. Non‐toxic potentiation of cancer chemotherapy by combined C and K3 vitamin pre‐treatment. Int J Cancer 1987; 40: 575–579.
  • 15 . Casado-Zapico S, Rodriguez-Blanco J, García-Santos G, Martín V, Sánchez-Sánchez AM, Antolín I et al. Synergistic antitumor effect of melatonin with several chemotherapeutic drugs on human Ewing sarcoma cancer cells: Potentiation of the extrinsic apoptotic pathway. J Pineal Res 2010; 48: 72–80.
  • 16 . ERDEN Y, GÜNAY S. TÜMÖR HÜCRELERİ APOPTOZ FAKTÖRÜ (TCApF)’NİN İNSAN PROSTAT VE MEME KANSERİ HÜCRE HATLARI ÜZERİNE SİTOTOKSİK VE GENOTOKSİK ETKİLERİNİN BELİRLENMESİ. İnönü Üniversitesi Sağlık Hizmetleri Mesl Yüksek Okulu Derg 2020; 8: 356–366.
  • 17 . Kang. Implication of necrosis-linked p53 aggregation in acquired apoptotic resistance to 5-FU in MCF-7 multicellular tumour spheroids. Oncol Rep 2010; 24: 4163–8.
  • 18 . Dewanto V, Wu X, Liu RH. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 2002; 50: 4959–4964.
  • 19 . Wilson TA, Nicolosi RJ, Woolfrey B, Kritchevsky D. Rice bran oil and oryzanol reduce plasma lipid and lipoprotein cholesterol concentrations and aortic cholesterol ester accumulation to a greater extent than ferulic acid in hypercholesterolemic hamsters. J Nutr Biochem 2007; 18: 105–112.
  • 20 . Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55–63.
  • 21 . Tekin S, Sandal S, Colak C. Effects of Apelin-13 on Human Prostate Cancer Lines [Insan Prostat Kanseri Hucre Serilerinde Apelin-13’un Etkileri]. Med Sci | Int Med J 2014; 3: 1427.
  • 22 . Genc ZK, Tekin S, Sandal S, Sekerci M, Genc M. Synthesis and DFT studies of structural and some spectral parameters of nickel(II) complex with 2-(2-hydroxybenzoyl)-N-(1-adamantyl) hydrazine carbothioamide. Res Chem Intermed 2015; 41: 4477–4488.
  • 23 . Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184–191.
  • 24 . Erden Y. Sour black mulberry (Morus nigra L.) causes cell death by decreasing mutant p53 expression in HT-29 human colon cancer cells. Food Biosci 2021; 42: 101113.
  • 25 . Hosseini F, Sam MR, Jabbari N, Mozdarani H. Modulating Survivin as a Radioresistant Factor, Caspase-3, and Apoptosis by Omega-3 Docosahexaenoic Acid Sensitizes Mutant-p53 Colorectal Cancer Cells to γ-Irradiation. Cancer Biother Radiopharm 2018; 33: 387–395.
  • 26 . Sritharan S, Sivalingam N. Curcumin induced apoptosis is mediated through oxidative stress in mutated p53 and wild type p53 colon adenocarcinoma cell lines. J Biochem Mol Toxicol 2021; 35: 1–10.
  • 27 . Węglarz L, Molin I, Orchel A, Parfiniewicz B. colon cancer HT-29 cells treated with inositol hexaphosphate. 2006; 53: 349–356.
  • 28 . Yu JJ, Lee KB, Mu C, Li Q, Abernathy T V., Bostick-Bruton F et al. Comparison of two human ovarian carcinoma cell lines (A2780/CP70 and MCAS) that are equally resistant to platinum, but differ at codon 118 of the ERCC1 gene. Int J Oncol 2000; 16: 555–560.
  • 29 . Lee JG, Ahn JH, Kim TJ, Lee JH, Choi JH. Mutant p53 promotes ovarian cancer cell adhesion to mesothelial cells via integrin β4 and Akt signals. Sci Rep 2015; 5: 1–12.
  • 30 . Muthusamy G, Balupillai A, Ramasamy K, Shanmugam M, Gunaseelan S, Mary B et al. Ferulic acid reverses ABCB1-mediated paclitaxel resistance in MDR cell lines. Eur J Pharmacol 2016; 786: 194–203.
  • 31 . Muthusamy G, Gunaseelan S, Prasad NR. Ferulic acid reverses P-glycoprotein-mediated multidrug resistance via inhibition of PI3K/Akt/NF-κB signaling pathway. J Nutr Biochem 2019; 63: 62–71.
  • 32 . Saenglee S, Jogloy S, Patanothai A, Senawong T. Cytotoxic effects of peanut phenolic compounds possessing histone deacetylase inhibitory activity on human colon cancer cell lines. Turkish J Biol 2016; 40: 1258–1271.
  • 33 . Sarwar T, Zafaryab M, Husain MA, Ishqi HM, Rehman SU, Moshahid Alam Rizvi M et al. Redox cycling of endogenous copper by ferulic acid leads to cellular DNA breakage and consequent cell death: A putative cancer chemotherapy mechanism. Toxicol Appl Pharmacol 2015; 289: 251–261.
  • 34 . Gao J, Yu H, Guo W, Kong Y, Gu lina, Li Q et al. The anticancer effects of ferulic acid is associated with induction of cell cycle arrest and autophagy in cervical cancer cells. Cancer Cell Int 2018; 18: 1–9.
There are 34 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Research Articles
Authors

Hamdi Kamçı 0000-0001-9255-2125

Project Number NONE
Publication Date January 30, 2023
Submission Date March 22, 2022
Published in Issue Year 2023 Volume: 4 Issue: 1

Cite

EndNote Kamçı H (January 1, 2023) Potentiation of Cell Death and DNA Damage Through 5-Fluorouracil and Ferulic Acid Coadministration in p53 Mutant HT-29 Cell Lines. New Trends in Medicine Sciences 4 1 1–7.