PSATHYRELLA CONDOLLEANA VE PSATHYRELLA SPADICEOGRISEA'NIN ASPERGILLUS TÜRLERİ ÜZERİNDE ANTİFUNGAL AKTİVİTESİ

Year 2017, Volume: 12 Issue: 4, 42 - 47, 11.10.2017

HEMEN GÜL Eraslan , Perihan Güler

Abstract

Bu çalışmada, Psathyrella türleri (P. candolleana, P. spadiceogrisea)’nin patojen mikrofunguslar Aspergillus (A. niger, A. versicolor) üzerine antifungal etkileri incelenmiştir. A. niger, A. versicolor, P. candolleana, P. spadiceogrisea sırasıyla malt ekstrakt agar ve nutrient broth besiyerlerinde üretildi. Çalışmada çözgen olarak etanol ve metanol kullanıldı.  A. niger ve A. versicolor’dan ayrı ayrı olacak şekilde 1000 mikrolitre örnekler alınarak malt ekstrakt agar üzerine yayılarak kuruması beklendi. Üzerlerine disk difüzyon yöntemi ile steril distile su, etanol ve metanolde 48 saat

muamele edilen P.candolleana ve P. spadiceogrisea emdirilmiş diskler su-etanol ve su-metanol şeklinde 2cm aralıkla bırakıldı. Karanlıkta, 27oC’de, 48 saat inkübe edildi. Psathyrella candolleana’nın her iki çözgeninin her iki Aspergillus türüne karşı antifungal etkisi görülmedi. Psathyrella spadiceogrisea’nın her iki çözgeninin her iki Aspergillus türüne karşı antifungal etkisi olduğu belirlendi.

Keywords

Psathyrella candolleana, Aspergillus, Psathyrella spadiceogrisea, Antifungal Etki

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