BibTex RIS Cite

Deneysel Olarak Salmonella enterica Serovar Enteritidis ile Enfekte Edilen Broylerlerde Enrofloksasin İlavesinin Oksidatif Stres ve Antioksidan Enzim Aktiviteleri Üzerine Etkisi

Year 2009, Volume: 4 Issue: 2, 24 - 33, 01.03.2009

Fulya Benzer

Abstract

Bu çalışma, enrofloksasinin tavuklarda deneysel S. enteritidis enfeksiyonlarında oksidatif stres ve antioksidan enzim aktivitesi üzerine etkisini araştırmak amacıyla yapıldı. Araştırmada kullanılan broiler tavuklar her grupta 15 hayvan olacak şekilde 4 gruba ayrıldı. Birinci grup kontrol grubu olarak ayrılırken, grup 2, 3, 4'teki tavuklara sırasıyla tek doz ağız yoluyla (peros) S. enteritidis suşu (0.1 ml'sinde 109 bakteri ihtiva eden inokulumlar), tek doz S. enteritidis uygulamasını takiben 15. gününden itibaren 9 gün süreyle enrofloksasin (10mg/kg/gün), 9 gün süre ile tek başına enrofloksasin (10mg/kg/gün) içme sularına katıldı. İnokulasyonu takiben 3. günden başlamak üzere örnekler alınarak bakteriyolojik etken izolasyonu yapıldı. Uygulama sonunda kan ve doku örnekleri alınarak MDA, NO düzeyleri ile GSH-Px ve KAT aktiviteleri ölçüldü. Plazma MDA düzeyleri kontrole göre bütün gruplarda arttı, en fazla artış tek başına antibiyotik verilen grupta görüldü. Eritrosit KAT aktivitesi ve plazma NO düzeyi hiçbir grupta değişmezken, eritrosit GSH-Px aktivitesi her üç grupta da kontrole göre düştü. Karaciğer MDA düzeyi ve GSH-Px aktivitesi hiçbir grupta değişmedi. NO düzeyi sadece antibiyotik verilen gruplarda (Salmonella+Antibiyotik, Kontrol+Antibiyotik) arttı. KAT aktivitesi kontrole göre bütün gruplarda düştü. Bağırsak dokusunda GSH-Px aktivitesi hiçbir grupta değişiklik göstermedi. KAT aktivitesi ve NO düzeyi kontrole göre bütün gruplarda düşüş gösterdi. MDA düzeyi Salmonellalı ve antibiyotik verilen kontrol grubunda (Kontrol+Antibiyotik) arttı. Sonuç olarak bakteriyel enfeksiyon serbest radikal hasarını artırmış, bu artış antibiyotik uygulanan gruplarda daha belirgin olarak görülmüştür. Bu nedenle antibiyotik uygulamalarında antioksidan sistemi kuvvetlendirecek maddeler verilmesinde yarar vardır.

Keywords

S. enterica serovar Enteritidis ENROFLOKSASİN MDA NO GSH-PX VE KAT

INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS

Year 2009, Volume: 4 Issue: 2, 24 - 33, 01.03.2009

Fulya Benzer

Abstract

The objective of this study was to assess influence of enrofloxacin administration on oxidative stress and the antioxidant enzyme activities of experimentally infected broilers with S. enterica serovar Enteritidis. Broiler chickens were divided into 4 groups, each consisting of 15 animals. Group 1 was used as control group; Group 2 orally received a single dose of S. enterica serovar Enteritidis strain (109 bacteria in 0.1 ml); Group 3 orally received a single dose of S. enterica serovar Enteritidis strain (109 bacteria in 0.1 ml) and enrofloxacin (10mg/kg/day) in drinking water for 9 days beginning from 15th day post administration and Group 4 was administered enrofloxacin (10mg/kg/day) in drinking water for 9 days. Beginning from 3 day post infection, samples were collected and bacterial isolation was done. At the end of the experiment, blood and tissue samples were collected and MDA, NO, GSH-Px and CAT activities were determined. Plasma MDA levels increased in all study groups compared to controls, being highest in antibiotic-administered group. Erythrocyte CAT activity and levels of plasma NO did not change among groups whereas erythrocyte GSH-Px activity decreased in all study groups compared to controls. There were no change levels of MDA and GSH-Px activity in liver. Levels of NO increased only groups given antibiotic (Salmonella+Antibiotic and Control+Antibiotic). Activity of CAT decreased in all study groups compared to controls. Intestinal GSH-Px activity didn't change in all groups. Activity of CAT and levels of NO decreased in all groups compared the control group. Levels of MDA increased in control groups given antibiotic and salmonella group. As a result, to decrease oxidative damage taking place in regard to bacterial infection and using antibiotic, the substances making antioxidant system strong should be given.

Keywords

S. enterica serovar Enteritidis Enrofloxacin MDA NO GSH-PX AND CAT

There are 0 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Basic Sciences
Authors

Fulya Benzer This is me

Publication Date March 1, 2009
Published in Issue Year 2009 Volume: 4 Issue: 2

Cite

APA Benzer, F. (2009). INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS. Veterinary Sciences, 4(2), 24-33.
AMA Benzer F. INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS. Veterinary Sciences. March 2009;4(2):24-33.
Chicago Benzer, Fulya. “INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS”. Veterinary Sciences 4, no. 2 (March 2009): 24-33.
EndNote Benzer F (March 1, 2009) INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS. Veterinary Sciences 4 2 24–33.
IEEE F. Benzer, “INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS”, Veterinary Sciences, vol. 4, no. 2, pp. 24–33, 2009.
ISNAD Benzer, Fulya. “INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS”. Veterinary Sciences 4/2 (March 2009), 24-33.
JAMA Benzer F. INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS. Veterinary Sciences. 2009;4:24–33.
MLA Benzer, Fulya. “INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS”. Veterinary Sciences, vol. 4, no. 2, 2009, pp. 24-33.
Vancouver Benzer F. INFLUENCE OF ENROFLOXACIN ADMINISTRATION ON OXIDATIVE STRESS AND ANTIOXIDANT ENZYME ACTIVITIES OF EXPERIMENTALLY INFECTED BROILERS WITH SALMONELLA ENTERICA SEROVAR ENTERITIDIS. Veterinary Sciences. 2009;4(2):24-33.