Green Synthesis of Silver Nanoparticles Using Safran (Crocus sativus) Purple flower and Their Antimicrobial activity

Abstract

Objective: In this research, CS-AgNPs were created, described, and their antibacterial activity assessed utilizing an inexpensive, simple, and ecologically friendly extraction approach from the waste saffron flower's purple flower parts. Methods: : In this study, silver nanoparticles (AgNPs) were synthesized quickly, cheaply, and environmentally friendly utilizing purple flower extract from saffron (Crocus sativus (CS)). For the explanation of the structure of silver nanoparticles synthesized with saffron flower extract (CS-AgNPs); UV-visible (UV-vis.) Spectrophotometer, Fourier Scanning Electron Microscope (FE-SEM), Scanning Electron Microscope (SEM), Electron Distributed X-rays (EDX), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Characterized using Diffraction Diffractrometer (XRD), Transmission Electron Microscope (TEM), Zeta potential data. It was discovered that the UV-visible spectrum of the biologically produced silver nanoparticles generated at a maximal wavelength of 405.68 nm in the spectrum collected after 15 minutes. Furthermore, it was noted that the synthesized nanoparticles' UV spectrum, taken a month later, revealed the same wavelength and that the resulting nanoparticles were stable. CS-AgNPs' antimicrobial effects against gram-positive, gram-negative, and fungal pathogens were assessed employing the minimum inhibition concentration approach. Results: The generated CS-AgNPs were found to be active against both fungi and bacteria as a consequence. Conclusion: It turned out that even at very low concentrations, the antimicrobial activity of silver nanoparticles enhanced with decreasing size and had high antibacterial and anticandidal implementation.

Keywords

• Crocus sativus
• CS-AgNPs
• MIC
• UV-Vis and Antimicrobial effect

Safran (Crocus sativus ) Mor Çiçekleri Kullanılarak Gümüş Nanopartiküllerin Yeşil Sentezi ve Antimikrobiyal Aktiviteleri

Abstract

Amaç: Bu araştırmada, safran çiçeğinin mor çiçekleri parçalarından ucuz, basit ve çevre dostu bir ekstraksiyon yaklaşımı kullanılarak CS-AgNP'ler oluşturulmuş, tanımlanmış ve antibakteriyel aktiviteleri değerlendirilmiştir. Metod: Bu çalışmada saffrondan (Crocus sativus (CS)) elde edilen mor çiçek özü kullanılarak gümüş nanoparçacıklar (AgNP'ler) hızlı, ucuz ve çevre dostu olarak sentezlendi. Safran çiçeği özü (CS-AgNPs) ile sentezlenen gümüş nanopartiküllerin yapısının açıklanması için; UV-görünür (UV-vis.) Spektrofotometre, Fourier Taramalı Elektron Mikroskobu (FE-SEM), Taramalı Elektron Mikroskobu (SEM), Elektron Dağıtılmış X-ışınları (EDX), Fourier Dönüşümlü Kızılötesi Spektroskopi (FT-IR), X-Ray Kırınım Kırınım Ölçer (XRD), Geçirgen Elektron Mikroskobu (TEM), Zeta potansiyel verileri kullanılarak karakterize edilmiştir. Biyolojik olarak üretilen gümüş nanopartiküllerin UV-görünür spektrumunun 15 dakika sonra toplanan spektrumda maksimum 405.68 nm dalga boyunda üretildiği keşfedildi. Ayrıca sentezlenen nanoparçacıkların bir ay sonra alınan UV spektrumunun aynı dalga boyunu ortaya çıkardığı ve ortaya çıkan nanoparçacıkların kararlı olduğu kaydedildi. CS-AgNP'lerin gram-pozitif, gram-negatif ve mantar patojenlerine karşı antimikrobiyal etkileri, minimum inhibisyon konsantrasyonu yaklaşımı kullanılarak değerlendirildi. Bulgular: Çok düşük konsantrasyonlarda bile gümüş nanoparçacıkların antimikrobiyal aktivitesinin küçülen boyutla arttığı ve yüksek antibakteriyel ve antikandidal uygulamaya sahip olduğu ortaya çıktı.

Keywords

• Crocus sativus
• CS-AgNP
• MİK
• Antibakteriyel etki ve Antifungal etki

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