Deneysel Olarak İntraabdominal Sepsis Oluşturulan Ratlarda Amiodaronun Böbrekler Üzerindeki Koruyucu Etkilerinin Değerlendirilmesi

Abstract

Amaç: Bu çalışmada, sıçanlarda intraabdominal sepsis deney modelinde amiodaronun böbrek koruyucu potansiyeli araştırılmıştır.

Gereç ve Yöntemler: Toplam 100 erkek Wistar sıçanı beş gruba ayrılmıştır: sham cerrahi uygulanan bir kontrol grubu; sham cerrahi uygulanan ve 50 mg/kg dozunda amiodaron verilen bir kontrol grubu; çekal ligasyon ve perforasyon (CLP) uygulanan bir grup; CLP uygulanan ve 25 mg/kg dozunda amiodaron verilen bir grup; ve CLP uygulanan ve 50 mg/kg dozunda amiodaron verilen bir grup. Sepsis modelini oluşturmak için CLP tekniği kullanılmış ve amiodaron, ameliyat sırasında bir tüp aracılığıyla ağız yoluyla verilmiştir.

Bulgular: CLP ile indüklenen sepsis, kreatinin, üre, malondialdehit, IL-1β mRNA ve TNF-α mRNA düzeylerinde anlamlı bir artışa neden olmuştur; buna paralel olarak, süperoksit dismutaz ve glutatyon düzeylerinde anlamlı bir azalma gözlenmiştir. Amiodaron, bu değişiklikleri doza bağlı bir şekilde tersine çevirdi. 50 mg/kg'lık doz, tüm biyokimyasal, moleküler ve histolojik parametrelerde en kapsamlı iyileşmeye yol açtı.

Sonuç: Sonuçlar, amiodaronun sepsisle ilişkili akut böbrek hasarını hafifletebileceğini göstermektedir. Bu etki muhtemelen ilacın antioksidan ve antienflamatuar özelliklerinin birleşimi yoluyla ortaya çıkmaktadır.

Keywords

• sepsis
• akut böbrek yetmezliği
• amiodaron
• aksidatif stres
• inflamasyon
• hayvan

Supporting Institution

Bu çalışma Atatürk Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir.

Ethical Statement

Bu çalışma için Atatürk Üniversitesi Tıp Fakültesi Yerel Hayvan Etik Kurulu'ndan 31.05.2013 tarihli ve 1/16 sayılı etik onay alınmış ve çalışma Helsinki Bildirgesi kurallarına uygun olarak yürütülmüştür.

Evaluation of the Protective Effects of Amiodarone on the Kidneys in Rats with Experimentally Induced Intra-Abdominal Sepsis

Abstract

Objective: This study examined the renoprotective potential of amiodarone in an experimental rat model of intra-abdominal sepsis.

Materials and Methods: A total of 100 male Wistar rats were distributed into five groups: sham-operated controls, sham controls receiving amiodarone 50 mg/kg, cecal ligation and puncture (CLP), CLP with amiodarone 25 mg/kg, and CLP with amiodarone 50 mg/kg. The CLP technique was employed to establish the sepsis model, and amiodarone was delivered orally via gavage at the time of surgery.

Results: Sepsis induction via CLP caused significant increases in creatinine, urea, malondialdehyde, IL-1β mRNA, and TNF-α mRNA, accompanied by significant decreases in superoxide dismutase and glutathione. Amiodarone reversed these alterations in a dose-dependent manner, with the 50 mg/kg dose producing the most comprehensive improvements across all biochemical, molecular, and histological measures.

Conclusion: The results suggest that amiodarone may mitigate sepsis-associated acute kidney injury, an effect likely mediated through its combined antioxidant and anti-inflammatory properties.

Keywords

• sepsis
• acute kidney injury
• amiodarone
• oxidative stress
• inflammation
• animal

Supporting Institution

This study was supported by Atatürk University Scientific Research Projects Coordination Unit

Ethical Statement

Ethical permission was obtained from the Atatürk University, Medical Faculty Local Animal Ethics Committee for this study with date 31.05.2013 and number 1/16 and Helsinki Declaration rules were followed to conduct this study.

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