Extraction and Purification of Eriocitrin from Mentha piperita

Year 2024, Volume: 9 Issue: 2, 119 - 128, 29.12.2024

Duygu Mısırlı , Merve Soy , Zafer Ömer Özdemir , Mahfuz Elmastas

https://doi.org/10.56171/ojn.1524775

Abstract

Currently, the practice of using plants for treatment is a multidisciplinary field referred to as "phytotherapy." The presence of secondary metabolites in plants is one of the main reasons for their utilization in the pharmaceutical sector. Research assessing the scientific use of plants utilized in Traditional and Complementary Medicine (GETAT) inside modern medicine would enhance the healthcare system by delivering highly efficient pharmacological molecules with minimal adverse effects, potentially stimulating the country's economy. In this article, the levels of 16 specific phenolic compounds in Mentha piperita, Mentha dumoretum, Mentha spicata and Mentha villosa nervata were investigated and the purification of eriocitrine from Mentha piperita was attempted. Eriocitrin is a secondary metabolite that has been shown to have various benefits for human health. The process of purifying bioflavonoid combinations containing Eriocitrin from local natural sources is becoming increasingly important. The HPLC analysis revealed that the Butanol extract of Mentha piperita contained the highest concentrations of eriocitrin. Therefore, the purification process was carried out utilizing these extracts obtained from Mentha piperita. This research has yielded eriocitrin with a purity of 92%.

Keywords

Extraction, Mentha species, Chromatography, Eriocitrin

Ethical Statement

This work has received financial support from the Scientific research projects Coordination of University of Health Sciences Turkey under Project Number 2022/47.

Supporting Institution

Scientific research projects Coordination of University of Health Sciences Turkey

Project Number

2022/047

Thanks

The authors thank the Scientific Research Projects Coordination Unit of the University of Health Sciences Turkey for financial support.

Mentha piperita'dan Eriositrin'in Ekstraksiyonu ve Saflaştırılması

Abstract

Günümüzde, bitkileri tedavi amaçlı kullanma uygulaması "fitoterapi" olarak adlandırılan çok disiplinli bir alandır. Bitkilerdeki sekonder metabolitlerin varlığı, bunların ilaç sektöründe kullanılmasının başlıca nedenlerinden biridir. Geleneksel ve Tamamlayıcı Tıpta (GETAT) kullanılan bitkilerin modern tıpta bilimsel kullanımını değerlendiren araştırmalar, en az yan etkiye sahip yüksek verimli farmakolojik moleküller sunarak sağlık sistemini geliştirecek ve potansiyel olarak ülke ekonomisini canlandıracaktır. Bu makalede Mentha piperita, Mentha dumoretum, Mentha spicata ve Mentha villosa nervata'daki 16 spesifik fenolik bileşiğin düzeyleri araştırılmıştır ve Mentha piperita'dan eriositrin saflaştırılmasına çalışılmıştır. Eriositrin insan sağlığı için çeşitli faydaları bulunduğu gösterilmiş bir sekonder metabolittir. Yerel doğal kaynaklardan Eriositrin içeren biyoflavonoid kombinasyonlarının saflaştırılması süreci giderek daha önemli hale gelmektedir. HPLC analizi, Mentha piperita'nın Butanol özütünün en yüksek eriocitrin konsantrasyonlarını içerdiğini ortaya koymuştur. Bu nedenle, saflaştırma işlemi Mentha piperita'dan elde edilen bu özütler kullanılarak gerçekleştirilmiştir. Bu araştırma sonucunda %92 saflıkta eriositrin elde edilmiştir.

Keywords

Ekstraksiyon, Mentha türleri, Kromatografi, Eriositrin

Ethical Statement

Bu çalışma Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'nin 2022/47 Proje Numarası ile finansal destek almıştır.

Supporting Institution

Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2022/047

Thanks

Yazarlar finansal desteği için Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimine teşekkür ederler.

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