Hydrogen peroxide-induced oxidative stress and apoptosis in SH-SY5Y cells: Protective effect of Momordica charantia fruit extract

Abstract

Oxidative stress triggers apoptosis in neuronal cells, resulting in cellular loss, which is critical in the pathogenesis of neurodegenerative diseases. Momordica charantia (MC), a traditional medicinal plant, is famous for its variety of health benefits, including its anti-diabetic, anti-inflammatory, and antioxidant properties. The purpose of this study was to investigate on how MC might affect oxidative stress and apoptosis caused by H2O2. First, we investigated whether ethanol extract of MC in the presence of H2O2 attenuated cell death in human neuroblastoma SH-SY5Y cells. MC improved H2O2-stimulated intracellular reactive oxygen species (ROS) production. Additionally, MC reduced caspase activation, which greatly improved cell viability and avoided H2O2-induced apoptosis. Through its anti-oxidant and anti-apoptotic properties, MC ethanol extract has been shown to be effective in protecting against H2O2-induced cell death.

Keywords

• Hydrogen peroxide
• Oxidative stress
• Apoptosis

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