Affordable and Simple Protocol for Immunofluorescence Staining of Insulin Secreting iGL Cell Line 2D and 3D

Abstract

Immunofluorescence staining is a widely used technique to visualize the localization of specific proteins in cells. This protocol provides an affordable and simple method for immunofluorescence staining of the Insulin-GLase (iGL) cell line in both 2D and 3D culture conditions. We describe cost-effective alternatives for reagents and materials without compromising the quality of the results, such as the use of Bovine Serum Albumin (BSA) 3% as a blocking buffer instead of normal serum. Immunofluorescence staining on the iGL cell line is a valuable technique for visualizing the distribution and localization of target biomolecules. With careful consideration of cost-effective strategies and an understanding of the unique characteristics of the iGL cell line, it is possible to achieve reliable and informative immunofluorescence results even with budget constraints.

Keywords

• Cell culture
• Immunofluorescence
• iGL
• 2D and 3D culture

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