Sıçanlarda Siyatik Sinir Yaralanma Modelinde Lavandula angustifolia'nın Periferik Sinirler Üzerine Etkisi

Abstract

Bu çalışma, Lavandula angustifolia'nın periferik sinir hasarı üzerindeki etkisini değerlendirmeyi amaçlamaktadır. Yirmi dört erkek Sprague-Dawley sıçanı rastgele üç gruba ayrıldı. Kan örneklerinden bazal inflamatuar belirteçler ölçüldü. Kontrol grubu (C) herhangi bir cerrahi işlem geçirmedi, ancak serum fizyolojik (SF) ve Lavandula (L) gruplarına cerrahi müdahale uygulandı. Açığa çıkarılan siyatik sinire 3/0 prolene sütür kullanılarak 300 gramlık bir traksiyon uygulandı. SF grubuna günlük 30 mg/kg serum fizyolojik verilirken, L grubuna 1:5 oranında hazırlanmış Lavandula-metanol solüsyonu uygulandı. Postoperatif 1., 2. ve 6. haftalarda elektromiyografi (EMG) yapıldı ve inflamatuar belirteçler için tekrar kan örnekleri alındı. Gecikme süresi açısından SF ve L grupları arasında anlamlı fark gözlenmedi; ancak amplitüd, SF grubunda (590) L grubuna kıyasla anlamlı şekilde daha düşüktü. İnflamatuar belirteçler (AGE, APOPP, DT, SOD, LHP ve TBARS), C ve SF grupları arasında anlamlı farklılık gösterdi (p < 0.05), ancak C ve L grupları arasında anlamlı fark gözlenmedi. C ve L gruplarında vasküler konjesyon ile birlikte bağ dokusu birikimi gözlemlendi ve trikom boyama ile fibrozis doğrulandı. L. angustifolia, antiinflamatuar, antioksidan ve nörojenik özellikleri sayesinde sinir rejenerasyonunu destekleyici potansiyel göstermektedir ve bu nedenle ileri klinik çalışmalara ihtiyaç vardır.

Keywords

• periferik sinir yaralanması
• siyatik sinir yaralanması
• lavanta
• Lavandula angustifolia
• sinir rejenerasyonu

Project Number

2022.09.04

The effect of lavandula angustifolia on peripheral nerves in a sciatic nerve injury model in rats

Abstract

Peripheral nerve injuries remain a significant clinical challenge due to incomplete functional recovery and persistent oxidative and inflammatory damage following trauma. Lavandula angustifolia, known for its anti-inflammatory and antioxidant properties, has demonstrated neuroprotective effects in experimental central nervous system models; however, its role in peripheral nerve regeneration has not been fully elucidated. The present study investigated the effects of orally administered Lavandula angustifolia extract in a standardized rat sciatic nerve traction injury model. Twenty-four male Sprague–Dawley rats were randomly assigned to sham-operated, injury + saline, and injury + Lavandula groups. A controlled traction force of 300 g was applied to the exposed sciatic nerve for 60 seconds using a calibrated weight-pulley system. The Lavandula extract was prepared via methanolic extraction with complete solvent evaporation and reconstitution in saline prior to oral administration (30 mg/kg/day). Electrophysiological evaluations were performed at weeks 1, 2, and 6, and serum oxidative stress markers were analyzed. At six weeks, the Lavandula-treated group demonstrated significantly higher compound muscle action potential amplitudes compared to the saline group, while latency values were comparable. Oxidative stress markers, including advanced oxidation protein products and lipid peroxidation indices, were significantly elevated in the saline group but attenuated in the Lavandula group. Superoxide dismutase levels were significantly increased following Lavandula treatment and positively correlated with electrophysiological recovery. Histopathological examination revealed higher axonal density and reduced fibrosis in the treatment group. These findings suggest that Lavandula angustifolia enhances functional and structural recovery following peripheral nerve traction injury, likely through antioxidant-mediated neuroprotective mechanisms. Further experimental and translational studies are warranted to clarify dose-response relationships and clinical applicability.

Keywords

• peripheral nerve injury
• sciatic nerve injury
• lavendula
• lavendula angustifolia
• nerve regeneration

Project Number

2022.09.04

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