Sıçan (Wistar albino) merkezi sinir sisteminde kadmiyum sülfatla (CdSO4) birlikte verilen nitrik oksit sentaz (NOS) inhibitörlerinin toplam lipid ve protein miktarlarına etkisi

Year 2011, , 175 - 181, 27.01.2012

Mehmet Önger , Hayri Genç , Faruk Tan

https://doi.org/10.5835/jecm.omu.28.04.010

Abstract

Sıçanlarda (Wistar albino) somatomotor kortekse kadmiyum sülfat (CdSO4) verildikten sonra spesifik Nitrik Oksit Sentaz (iNOS) enzim inhibitörü olan Aminoguanidin (AG) ve NO öncüsü olan L-Arjinin (L-Arg)’in beyin ve beyincikteki toplam lipid ve protein miktarlarına etkisini tespit etmek üzere, sunulan çalışma planlandı. Bu amaçla, saf, kontrol, kadmiyum, kadmiyum+L-Arg ve kadmiyum+AG olmak üzere 7’şer sıçandan oluşan 5 grup oluşturuldu. Kadmiyum grubu sıçanlara 0,0021 mg/kg CdSO4 sol hemisfere (bregmanın lateraline) intrakortikal yoldan enjekte edildi. Kontrol grubuna aynı hacimde serum fizyolojik verildi. Kadmiyum+AG grubuna günde iki kez olmak üzere 100 mg/ kg AG 15 gün süreyle intraperitoneal yoldan verildi. Kadmiyum+L-Arjinin grubuna da günde iki kez olmak üzere 1000 mg/kg L-Arjinin 15 gün süreyle intraperitoneal yoldan verildi. Saf grup ise hiçbir işleme tabi tutulmadı ve hiçbir madde verilmedi.
Elde edilen sonuçlar, çalışılan diğer gruplara göre kadmiyumun toplam lipid ve protein miktarlarını düşürdüğünü ve toksik etkiye sahip olduğunu göstermektedir. Ayrıca, sunulan çalışma ile sıçanda kadmiyumun bu nörotoksik etkisinin, spesifik NOS inhibitörü olan AG tarafından azaltıldığına dair sonuçlar yeni bulgulardır. L-Arjininin kadmiyumdan kaynaklanan toplam lipid ve protein miktarlarındaki azalmayı arttırdığını ve bu nörotoksik etkide NO’nun da rol aldığını, elde ettiğimiz bulgular göstermektedir. 

Keywords

Cadmium Sulphate, Cerebrum, Cerebellum, Total Lipid, Total Protein, Aminoguanidine, L-Arginine, Rat

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