Penisilinle oluşturulan epilepside kolinerjik ve nitrerjik maddelerin diken amplitüdüne etkisi The effects of cholinergic and nitrergic substances on spike amplitude in penicillin induced epilepsy

Abstract

Literatürde asetilkolinin (ACh) ve nitrik oksitin (NO) epilepsideki yeriyle ilgili çalışmalar vardır. Bununla birlikte herhangi bir epilepsi modelinde muskarinik kolinerjik sistem ile nitrerjik sistem arasındaki etkileşim bilinmemektedir. Sunulan çalışmanın amacı, penisilin modeli deneysel epilepside, nitrerjik sistem ile muskarinik kolinerjik sistemin etkileşimini araştırmaktır. Deneylerde ağırlıkları ortalama 220±35 gram olan 70 tane Wistar cinsi erişkin erkek sıçan kullanıldı. Bu hayvanlar kontrol (200 IU/1µl penisilin), sodyum nitropurisid (SNP) (50 μg/5 μl), Nω-nitro-L-arjinin metil ester (L-NAME) (100 μg/5 μl), ACh (250 µg/5 µl, i.c.), atropin (100 ng i.c.), atropin+SNP (100 ng atropin ve 10 dk sonra 50 μg SNP i.c.), atropin+L-NAME (100 ng atropin sülfat ve 100 μg/5 μl L-NAME i.c.), L-NAME+ACh (100 μg/5 μl L-NAME ve 10 dk sonra 250 µg/5 µl ACh i.c.), ACh+SNP (250 µg/5µl ACh ve 50 μg SNP i.c.) ve Atropin+ACh gruplarına ayrıldı. Çalışmamızın sonuçlarına göre intrakortikal penisilinden (200 IU/1 mikro litre) 2-5 dakika sonra ECoG’de epileptiform aktivite başladı. Asetilkolin ile SNP birlikte verildiğinde, 10. dakikadan itibaren deneylerin sonuna kadar penisilinin oluşturduğu epileptiform aktivite ve diken yükseklikleri, istatistik açıdan çok önemli ölçüde baskılandı. Muskarinik kolinerjik ve nitrerjik sistemlerle ilgili diğer uygulamalar ve diğer maddeler diken yüksekliklerini istatistiksel açıdan önemli sayılabilecek ölçüde etkilememiştir. Sonuç olarak deney şartlarında, asetilkolin tek başına uygulandığında penisilinin oluşturduğu epileptiform aktiviteyi şiddetlendirmekte; fakat bir NO verici olan SNP ile birlikte verilince antikonvülzan etki artmakta ve diken yükseklikleri azalmaktadır. 

Keywords

• Asetilkolin
• Nitrik oksit
• ECoG
• Deneysel epilepsi
• Diken amplitüdü
• Sıçan

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