Analysis of Orosomucoid and C-Reactive Protein Levels in Gingival Tissue And Serum of Rats with Experimental Periodontitis: Comparison at different time points in disease progression

Abstract

Objectives: Acute-phase proteins are thought to trigger periodontal tissue destruction. The core purpose of this study is to analyze the levels of Orosomucoid1 (ORM1) and C-reactive protein (CRP) in gingival tissue and serum of rats with experimental periodontitis.

Material and Methods: Thirty rats were divided randomly and Group 1 was specified as control group, while the remaining groups were classified as ligature-induced experimental periodontitis groups. Each group was constituted 10 rats and Group 2 was evaluated the impact of ligature for the term of 7 days. Group 3 was classified on the basis of 14 days ligature. With the help of ELISA, gingival tissue and serum samples was utilized for measuring CRP and ORM1 levels.

Results: Alveolar bone and attachment loss was statistically higher in all experimental periodontitis groups than those in control group (P<0.001). The levels of CRP and ORM1 in gingival tissue were significantly higher in Group 2 than in Groups 1 and 3 (P<0.05). Also, a statistically significant positive correlation was found between CRP and ORM1 levels in the gingival tissue (P<0.001).

Conclusions: The present results reveal that tissue destruction at earlier periods of inflammatory periodontal disease may be associated with ORM. 

Keywords

• Orosomucoid; periodontitis; gingiva

References

1. Bain JL, Lester SR, Henry WD, Bishop CM, Turnage AA, Naftel JP, et al. Comparative gender differences in local and systemic concentrations of proinflammatory cytokines in rats with experimental periodontitis. J Periodont Res 2009;44:133–140.
2. Balli U, Keles GC, Cetinkaya BO, Mercan U, Ayas B, Erdogan D. Assessment of vascular endothelial growth factor and matrix metalloproteinase-9 in the periodontium of rats treated with atorvastatin. J Periodontol 2014;85:178-87.
3. Brito LC, DalBó S, Striechen TM, Farias JM, Olchanheski LR Jr, Mendes RT, et al. Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction. Arch Oral Biol 2013;58:1187-98.
4. Donatelli RE, Lee SJ. How to report reliability in orthodontic research: Part 1. Am J Orthod Dentofacial Orthop 2013;144,156-61.
5. Feng Z, Weinberg A. Role of bacteria in health and disease of periodontal tissues. Periodontol 2000 2006;40:50-76.
6. Fournier T, Medjoubi-N N, Porquet D. Alpha-1-acid glycoprotein. Biochim Biophys Acta 2000;1482:157-71.
7. Gornik O, Lauc G. Glycosylation of serum proteins in inflammatory diseases. Dis Markers 2008;25:267–278.
8. Gupta B, Sawhney A, Patil N, Yadav M, Tripathi S, Sinha S, et al. Effect of Surgical Periodontal Therapy on Serum C-reactive Protein Levels Using ELISA in Both Chronic and Aggressive Periodontitis Patient. J Clin Diagn Res 2015;9:ZC01-5.

9. Hochepied T, Berger FG, Baumann H, Libert C. Alpha(1)-acid glycoprotein: an acute phase protein with inflammatory and immunomodulating properties. Cytokine Growth Factor Rev Fujita 2003;14:25-34.
10. Keles GC, Balli U, Cetinkaya BO, Ayas B, Findik A, Keles ZP, et al. Biochemical analysis of pentraxin 3 and fibrinogen levels in experimental periodontitis model. Mediators Inflamm 2012;2012:809801.
11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275.
12. Lu Q, Jin L. Human gingiva is another site of C-reactive protein formation. J Clin Periodontol 2010;37:789-96.
13. Luo Z, Lei H, Sun Y, Liu X, Su DF. Orosomucoid, an acute response protein with multiple modulating activities. J Physiol Biochem 2015;71:329-40.
14. Maekawa T, Tabeta K, Kajita-Okui K, Nakajima T, Yamazaki K. Increased expression of C-reactive protein gene in inflamed gingival tissues could be derived from endothelial cells stimulated with interleukin-6. Arch Oral Biol 2011;56:1312-8.
15. Mahmoud FH, Abbas DK. Relationship between the Severity of Gingival inflammation and Concentration of Gingival Fluid Proteins. Journal of the College of Dentistry 2002;14:1-6.
16. Peruzzo DC, Benatti BB, Antunes IB, Andersen ML, Sallum EA, Casati MZ, et al. Chronic stress may modulate periodontal disease: a study in rats. J Periodontol 2008;79:697-704.
17. Rangé H, Poitou C, Boillot A, Ciangura C, Katsahian S, Lacorte JM, et al. Orosomucoid, a new biomarker in the association between obesity and periodontitis. PLoS One 2013;8:e57645.
18. Sai K, Kurose K, Koizumi T, Katori N, Sawada J, Matsumura Y, et al. Distal promoter regions are responsible for differential regulation of human orosomucoid-1 and -2 gene expression and acute phase responses. Biol Pharm Bull 2014;37:164-8.
19. Shimada Y, Komatsu Y, Ikezawa-Suzuki I, Tai H, Sugita N, Yoshie H. The effect of periodontal treatment on serum leptin, interleukin-6, and C-reactive protein. J Periodontol 2010;81:1118-23.
20. Sobaniec H, Sobaniec-Lotowska ME. Morphological examinations of hard tissues of periodontium and evaluation of selected processes of lipid peroxidation in blood serum of rats in the course of experimental periodontitis. Med Sci Monit 2000;6:875-81.
21. Vermeire S, Van Assche G, Rutgeerts P. Laboratory markers in IBD: useful, magic, or unnecessary toys? Gut 2006;55:426-31.