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The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle

Year 2001, Volume: 18 Issue: 3, - , 23.12.2009

Abstract

Mn2+ is an essential trace element, known as Ca2+ antagonist and suppress the action potential and contraction of various types of smooth muscle cells. It exerts its effects by entering the cells through L type Voltage-dependent Ca2+ channels, or receptor-operated Ca2+ channels and/or perhaps by other non selective cation channels. It may produce contraction by directly activating contractile proteins.
In the present study, we measured the Acetylcholine (ACh]-Induced (2.7xlO"7M) duodenal smooth muscle contractions containing standard and modified Tyrocle (Ca2+-free, and 2 fold Ca2+) batlı solutions with adding Atropine (1.4xl0~7 M), Diltiazem (5.5xl0"7 M) and MnCl2 (5 mM). In the experiments, rats (n=10) were used. Animals were killed by decapitation. Abdomen opened and duodenal pieces (1 cm) were taken. Their contents were flushed with ice-cold Tyrode solution. The duodenum pieces were suspended in an organ batlı containing 10 ml of Tyrode solution and aerated with 95% 02 and 5% C02, at 37 °C. After equilibration period of 30 minutes, contractile force was recorded isometrically with a force displacement transducer (Ugo-Basile 7003) connected to a microdynograph recorder (Ugo-Basile 7050). A resting tension of 0.3 g were used. At the three batlı solutions while atropine and diltiazem inhibited the ACh-induced contractions, MnCl2 (5 mM) induced a rapid and transient suppression of contractions. The average amplitudes of contractions with MnCl2 were significantly lower than those of diltiazem and atropine.
The results suggested that the suppression of contractions with Mn(( influx may due to other factors such as cAMP content and its effects on contractile elements



Manganklorürün İzole Sıçan Duodenum Düz Kasında Asetilkolinle İndüklenmiş Kontraksiyonlar Üzerine Etkisi

Manganez, iki değerlikli formu ile Ca+2 antagonistidir. Düz kasta aksiyon potansiyeli ve kontraksiyonu inhibe edebilir. Hücre içine girişi voltaj bağımlı L tipi Ca kanalı, reseptör bağımlı Ca kanalı ve/veya non selektif iyon kanalları aracılığı ile olmaktadır. Bu nedenle çeşitli organ düz kaslarında kontraktil proteinlere direkt etki ile kontraksiyon oluşturabileceği bildirilmektedir. Sunulan çalışmada standart, 2xCa+2 ve Ca2+"suz ortamlarda 2.7xl0"7 M ACh ile oluşturulan duodenum kontraktilitesi üzerine MnCl2 (5 mMJ'ün etkisi araştınldı. Diltiazem, Atropin ile oluşan kontraksiyon yükseklikleri ile karşılaştırıldı. Çalışmada 10 adet rat kullanıldı. Uygun şekilde izole edilen duodenal düz kas örneklerinde kontraksiyon yükseklikleri Ugo Basile izometrik force transdüseri yardımı ile yazdırıldı. Kontraksiyonlar atropin ve diltiazemle inhibe olurken, MnCl2 ile ani olarak kayboldu. Bu sonuç manganez etkisi ile oluşacağı bildirilen düz kas kontraktilitesinde hücre içi diğer faktörlerin de, örneğin; cAMP düzeyinin de rol oynayabileceğini düşündürdü.

Year 2001, Volume: 18 Issue: 3, - , 23.12.2009

Abstract

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Details

Primary Language English
Journal Section Basic Medical Sciences
Authors

N. Zanoğlu This is me

E. Koç This is me

G. Yıldırım This is me

Publication Date December 23, 2009
Submission Date November 16, 2009
Published in Issue Year 2001 Volume: 18 Issue: 3

Cite

APA Zanoğlu, N., Koç, E., & Yıldırım, G. (2009). The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle. Journal of Experimental and Clinical Medicine, 18(3). https://doi.org/10.5835/jecm.v18i3.284
AMA Zanoğlu N, Koç E, Yıldırım G. The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle. J. Exp. Clin. Med. December 2009;18(3). doi:10.5835/jecm.v18i3.284
Chicago Zanoğlu, N., E. Koç, and G. Yıldırım. “The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle”. Journal of Experimental and Clinical Medicine 18, no. 3 (December 2009). https://doi.org/10.5835/jecm.v18i3.284.
EndNote Zanoğlu N, Koç E, Yıldırım G (December 1, 2009) The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle. Journal of Experimental and Clinical Medicine 18 3
IEEE N. Zanoğlu, E. Koç, and G. Yıldırım, “The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle”, J. Exp. Clin. Med., vol. 18, no. 3, 2009, doi: 10.5835/jecm.v18i3.284.
ISNAD Zanoğlu, N. et al. “The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle”. Journal of Experimental and Clinical Medicine 18/3 (December 2009). https://doi.org/10.5835/jecm.v18i3.284.
JAMA Zanoğlu N, Koç E, Yıldırım G. The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle. J. Exp. Clin. Med. 2009;18. doi:10.5835/jecm.v18i3.284.
MLA Zanoğlu, N. et al. “The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle”. Journal of Experimental and Clinical Medicine, vol. 18, no. 3, 2009, doi:10.5835/jecm.v18i3.284.
Vancouver Zanoğlu N, Koç E, Yıldırım G. The Effects Of Manganase Chloride Onacetylcholine-Induced Contractions Of Isolated Rat Duodenal Smooth Muscle. J. Exp. Clin. Med. 2009;18(3).