Year 2018, Volume 35, Issue 4, 101 - 109, 18.11.2019

Muhammet Volkan BÜLBÜL Hanefi ÖZBEK Ozlem BAHADIR ACIKARA Serkan OZBILGIN Burcın ERGENE OZ Ekın KURTUL İlknur KESKIN

Abstract

Antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT- 116 cells

Abstract

In this study, the cytotoxic effects of the extracts of methanol: water (80:20) prepared from the above-ground parts of the varieties of Onobrychis albiflora Hub.-Mor., Onobrychis argyrea Boiss. subsp. argyrea, Onobrychis galegifolia Boiss. and Onobrychis tournefortii (Willd.) Desv. species to HCT-116 cells were investigated. With cytotoxicity analysis that with these species inhibitor concentrations (IC50) which resulted in a 50% reduction in the proliferation of HCT-116 cells were identified. In continuation of the study; the antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT-116 cells were evaluated by Caspase 3, Annexin V / PI Apoptosis / Necrosis analysis, Apopxin Green and 7-AAD Apoptosis / Necrosis analysis. The antiproliferative, apop- totic and necrotic effects of Onobrychis albiflora extract on HCT-116 cells also were determined. 

Keywords

HCT-116, FACS, HEK-293, NECROSİS, APOPTOSİS, COLON CANCER

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