Antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT- 116 cells

Year 2018, Volume: 35 Issue: 4, 101 - 109, 18.11.2019

Muhammet Volkan Bülbül Hanefi Özbek Ozlem Bahadır Acıkara Serkan Ozbılgın Burcın Ergene Oz Ekın Kurtul İlknur Keskın

Abstract

In this study, the cytotoxic effects of the extracts of methanol: water (80:20)
prepared from the above-ground parts of the varieties of Onobrychis albiflora
Hub.-Mor., Onobrychis argyrea Boiss. subsp. argyrea, Onobrychis galegifolia
Boiss. and Onobrychis tournefortii (Willd.) Desv. species to HCT-116 cells
were investigated. With cytotoxicity analysis that with these species inhibitor
concentrations (IC50) which resulted in a 50% reduction in the proliferation of
HCT-116 cells were identified. In continuation of the study; the antiproliferative
and apoptotic effects of Onobrychis albiflora extract on HCT-116 cells were
evaluated by Caspase 3, Annexin V / PI Apoptosis / Necrosis analysis, Apopxin
Green and 7-AAD Apoptosis / Necrosis analysis. The antiproliferative, apop-
totic and necrotic effects of Onobrychis albiflora extract on HCT-116 cells also
were determined. 

Keywords

HCT-116, FACS, HEK-293, NECROSİS, APOPTOSİS, COLON CANCER

Project Number

Bap 2016/18

References

• Acikgoz, E. 1988. Annual forage legumes in the arid and semi-arid regions of Turkey. In Nitrogen fixation by legumes in Mediterranean agriculture. Springer. pp. 47-54.
• Acıkgöz, E., 2001. Yem Bitkileri. Uludağ Üniv. Güçlendirme Vakfı Yayın. 182.
• Anbalagan, V., Raju, K., Shanmugam, M., 2017. Assessment of Lipid Peroxidation and Antioxidant Status in Vanillic Acid Treated 7,12-Dimethylbenz[a]anthracene Induced Hamster Buccal Pouch Carcinogenesis. Journal of clinical and diagnostic research. 11.3.
• Avci, S., Tekin, N., Sancak, C., Özcan, S., Maraghi, A.O., 2016. Phylogenetic relationship of some Onobrychis taxa naturally grown in Turkey based on morphology and nuclear ribosomal DNA ITS Sequences. Legume Research. 39(5), 665-673.
• Basli, A., Belkacem, N., Amrani, I., 2017. Health benefits of phenolic compounds against cancers. Phenolic compounds-biological activity. London: InTechOpen. 193-210.
• Bates, S.H., Jones, R.B., Bailey, C.J., 2000. Insulin‐like effect of pinitol. British journal of pharmacology. 130(8), 1944-1948.
• Choi, E.M., Hwang, J.K., 2004. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia. 75(6), 557-565.
• Conforti, F., Sosa, S., Marrelli, M., Menichini, F., Statti, G A., Uzunov, D., Della Loggia, R., 2008. In vivo anti-inflammatory and in vitro antioxidant activities of Mediterranean dietary plants. Journal of ethnopharmacology. 116(1), 144-151.
• Cragg, G M., Kingston, D.G., Newman, D.J., 2005. Anticancer agents from natural products. CRC press. pp. 89-122.
• Cragg, G M., Kingston, D.G., Newman, D.J., 2012. Anticancer agents from natural products. CRC press. pp. 123-175.
• Desai, A.G., Qazi, G.N., Ganju, R.K., El-Tamer, M., Singh, J., Saxena, A.K., Bhat, H.K., 2008. Medicinal plants and cancer chemoprevention. Current drug metabolism. 9(7), 581-591.
• Eitsuka, T., Tatewaki, N., Nishida, H., Nakagawa, K., Miyazawa, T., 2016. A combination of δ-tocotrienol and ferulic acid synergistically inhibits telomerase activity in DLD-1 human colorectal adenocarcinoma cells. Journal of nutritional science and vitaminology. 62(5), 281-287.
• Gonçalves, C., Dinis, T., Batista, M.T., 2005. Antioxidant properties of proanthocyanidins of Uncaria tomentosa bark decoction: a mechanism for anti-inflammatory activity. Phytochemistry. 66(1), 89-98.
• Guha, G., Rajkumar, V., Mathew, L., KUMAR, R.A., 2011. The antioxidant and DNA protection potential of Indian tribal medicinal plants. Turkish Journal of Biology. 35(2), 233-242.
• Halabalaki, M., Aligiannis, N., Papoutsi, Z., Mitakou, S., Moutsatsou, P., Sekeris, C., Skaltsounis, A.L., 2000. Three new arylobenzofurans from O n obrychis e benoides and evaluation of their binding affinity for the estrogen receptor. Journal of natural products. 63(12), 1672-1674.
• Hashemzaei, M., Delarami Far, A., Yari, A., Heravi, R.E., Tabrizian, K., Taghdisi, S.M., Nikitovic, D., 2017. Anticancer and apoptosis inducing effects of quercetin in vitro and in vivo. Oncology reports. 38(2), 819-828.
• Karamian, R., Asadbegy, M., 2016. Antioxidant activity, total phenolic and flavonoid contents of three Onobrychis species from Iran. Pharmaceutical sciences. 22(2), 112.
• Katsanou, E.S., Halabalaki, M., Aligiannis, N., Mitakou, S., Skaltsounis, A.L., Alexi, X., Alexis, M.N., 2007. Cytotoxic effects of 2-arylbenzofuran phytoestrogens on human cancer cells: modulation by adrenal and gonadal steroids. The Journal of steroid biochemistry and molecular biology. 104(3-5), 228-236.
• Korkmaz, F., 2010. Isırgan Otu (Urtica dioica) Ekstresinin Kolon Kanseri Kolon Kanseri Hücre Serileri Üzerindeki Apoptotik, Antiproliferatif ve Antioksidan Etkilerinin Araştırılması. A.Ü. Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi. 45-46.
• Lee, K.H., Xiao, Z., 2005. Podophyllotoxins and analogs. In Anticancer agents from natural products. CRC Press. 80-96.
• Lee, K.H., Xiao, Z., 2012. Podophyllotoxins and analogs. In Anticancer agents from natural products. CRC Press. 95-122.
• Marais, J.P., Mueller-Harvey, I., Brandt, E.V., Ferreira, D. 2000. Polyphenols, condensed tannins, and other natural products in Onobrychis viciifolia (sainfoin). Journal of Agricultural and Food Chemistry. 48(8), 3440-3447.
• Nicodemus, K.K., Jacobs, D.R., Folsom, A.R., 2001. Whole and refined grain intake and risk of incident postmenopausal breast cancer (United States). Cancer Causes & Control. 12(10), 917-925.
• Pang, J.H.S., Yen, J.H., Wu, H.T., Huang, S.T., 2017. Gallic acid inhibited matrix invasion and AP-1/ETS-1-mediated MMP-1 transcription in human nasopharyngeal carcinoma cells. International journal of molecular sciences. 18(7), 1354.
• Roy, N., Narayanankutty, A., Nazeem, P.A., Valsalan, R., Babu, T. D., Mathew, D., 2016. Plant phenolics ferulic acid and p-coumaric acid inhibit colorectal cancer cell proliferation through EGFR down-regulation. Asian Pac J Cancer Prev. 17(8), 4019-4023.
• Scarpa, E.S., Antonini, E., Palma, F., Mari, M., Ninfali, P., 2018. Antiproliferative activity of vitexin-2-O-xyloside and avenanthramides on CaCo-2 and HepG2 cancer cells occurs through apoptosis induction and reduction of pro-survival mechanisms. European journal of nutrition. 57(4), 1381-1395.
• Tyszka-Czochara, M., Bukowska-Strakova, K., Majka, M., 2017. Metformin and caffeic acid regulate metabolic reprogramming in human cervical carcinoma SiHa/HTB-35 cells and augment anticancer activity of Cisplatin via cell cycle regulation. Food and Chemical Toxicology. 106, 260-272.
• Veeresham, C., 2012. Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res. 200-201.
• World Health Organization, 2014. Cancer country profiles 2014. Geneva: World Health Organization.
• Zheng, A.W., Chen, Y.Q., Zhao, L.Q., Feng, J.G., 2017. Myricetin induces apoptosis and enhances chemosensitivity in ovarian cancer cells. Oncology letters. 13(6), 4974-4978.
• Zhu, B., Shang, B., Li, Y., Zhen, Y., 2016. Inhibition of histone deacetylases by trans-cinnamic acid and its antitumor effect against colon cancer xenografts in athymic mice. Molecular medicine reports. 13(5), 4159-4166.
• Zhu, K.C., Sun, J.M., Shen, J.G., Jin, J.Z., Liu, F., Xu, X.L., Lv, J.J., 2015. Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1. Oncology letters. 10(4), 2359-2365.