Evaluation of protective effects of mirtazapine and mesna on cisplatin-induced ovarian damage in rats

Year 2021, Volume: 38 Issue: 2, 72 - 81, 03.04.2021

Ali Doğukan Anğın , Muhammet Ali Oruç , Önder Sakin , Emine Eda Akalın , Muzaffer Çıkman , Kayhan Başak , Yasemin Alan , Asuman Orçun , Murat Alan

Abstract

Evaluation of protective effects of mirtazapine and mesna on cisplatin-induced ovarian damage in rats
Objective:
To evaluate whether mirtazapine and mesna have protective effects on cisplatin-induced ovarian injury.
Methods:
A total of 32 female Wistar Albino rats were divided into 4 groups (8 rats per group) and included in the study. No medication was administered to the first group; only intervention was that their ovaries were removed and anti-mullerian hormone (AMH) values were measured. The second group received intramuscular cisplatin at a single dose of 7.5 mg/kg. The third group received a single dose of 200 mg/kg mesna intraperitoneally, and 30 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. The fourth group received oral 30 mg/kg mirtazapine, and 60 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. Oral 30 mg/kg mirtazapine was continued for ten days. Ovaries and AMH values of all groups were evaluated at the end of tenth day.
Results:
In the cisplatin group when compared to normal ovarian tissue total histopathological damage score increased (p=0.037), preantral follicle count decreased (p=0.003) and AMH levels decreased (p<0.001). In the cisplatin+mesna group total ovarian damage score was also increased (p=0.005), preantral and antral follicles decreased (p<0.001 and p=0.001, respectively), and AMH levels decreased (p<0.001). In the cisplatin+mirtazapine group, total ovarian damage score (p <0.001), preantral follicle count (p = 0.002) and AMH values were decreased (p <0.001).
Conclusion:
It was concluded that mesna and mirtazapine were not effective in preventing ovarian damage due to cisplatin.

Keywords

Cisplatin, mirtazapine, mesna, anti-mullerian hormone, ovary, rats

References

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