Evaluation of serum levels of Copper and Selenium in H .pylori positive and negative gastritis patients

Year 2021, Volume: 38 Issue: 2, 182 - 186, 03.04.2021

Vahid Hamidi Mohammad Ranaei Nahid Neamati Javad Shokri Karimollah Hajian Abbas Mosapour Mehdi Soltanzadeh Mehdi Tourani Hadi Parsian

Abstract

Background: Helicobacter pylori (H. pylori) infection can result in various complications, included micronutrient variations, nutritional impairment, gastric tissue damages and oxidative stress. The present study aimed to evaluate serum variations of Cu and Se in H. pylori infected individuals.
Materials and methods: A prospective case-control study was performed on 69 participants with abdominal pains (40 patients with H. pylori gastritis versus 29 control group). Serum levels of Cu and Se were measured using Atomic Absorption Spectrophotometer. Statistical analysis was performed using SPSS software. Comparisons of elements between included groups were done using independent sample t-test. Subgroup analysis between male and female (with or without H. pylori) was performed using one way analysis of variance. Correlation between serum Cu and Se was assessed using Pearson’s correlation test. The p-value levels less than 0.05 were considered as significant.
Results: There were no significant differences in serum levels of Cu and Se between H. pylori positive and negative persons. In male with H. pylori, Se levels were 38.4% higher than control male (p-value=0.03). A positive and significant correlation was observed between serum Cu and Se levels (r = 0.51, P-value = 0.024).
Conclusion: Although no statistic difference was observed in serum Cu and Se levels, the significant correlation between measured elements represent as good evidence for some discrepancies. Given the importance of well controlled micronutrients in whole body health, especially in high risk H. pylori patients, more studies are needed to clarify the exact relation of these and other trace elements with H. pylori outcomes.

Keywords

Copper (Cu), Helicobacter pylori (H. pylori), Selenium (Se), gastroduodenal complication

Supporting Institution

The present study was funded by Babol University of Medical Sciences, Babol, Iran.

Project Number

4182

Thanks

The authors have also thanked vice chancellor for the approval of this study.

References

• 1. Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clinical microbiology reviews. 2006;19(3):449-90.
• 2. Eshraghian A. Epidemiology of Helicobacter pylori infection among the healthy population in Iran and countries of the Eastern Mediterranean Region: a systematic review of prevalence and risk factors. World journal of gastroenterology: WJG. 2014;20(46):17618.
• 3. Peleteiro B, Bastos A, Ferro A, Lunet N. Prevalence of Helicobacter pylori infection worldwide: a systematic review of studies with national coverage. Digestive diseases and sciences. 2014;59(8):1698-709.
• 4. Rezaeimehr Z, Hosseini SR, Darbandi Z, Hosseini SA, Kheirkhah F, Bijani A, et al. The association between helicobacter pylori infection and cognitive disorder in iranian elderly population. Archives of Clinical Infectious Diseases. 2016;11(4).
• 5. Ghadimi R, Taheri H, Suzuki S, Kashifard M, Hosono A, Esfandiary I, et al. Host and environmental factors for gastric cancer in Babol, the Caspian Sea Coast, Iran. European journal of cancer prevention. 2007;16(3):192-5.
• 6. Teimoorian F, Ranaei M, Tilaki KH, Shirvani JS, Vosough ZJIjop. Association of Helicobacter pylori Infection With Colon Cancer and Adenomatous Polyps. 2018;13(3):325.
• 7. Ding S-Z, Minohara Y, Fan XJ, Wang J, Reyes VE, Patel J, et al. Helicobacter pylori infection induces oxidative stress and programmed cell death in human gastric epithelial cells. Infection and immunity. 2007;75(8):4030-9.
• 8. Butcher LD, den Hartog G, Ernst PB, Crowe SE. Oxidative stress resulting from Helicobacter pylori infection contributes to gastric carcinogenesis. Cellular and molecular gastroenterology and hepatology. 2017;3(3):316-22.
• 9. Naito Y, Yoshikawa T. Molecular and cellular mechanisms involved in Helicobacter pylori-induced inflammation and oxidative stress1, 2. Free Radical Biology and Medicine. 2002;33(3):323-36.
• 10. Dovhanj J, Kljaic K, Vcev A, Ilakovac V. Helicobacter pylori and trace elements. Clinical laboratory. 2010;56(3-4):137-42.
• 11. Florianczyk B. Trace elements as constituents of antioxidative proteins. Journal of pre-clinical and clinical research. 2008;2(1).
• 12. Yakoob J, Jafri W, Abid S. Helicobacter pylori infection and micronutrient deficiencies. World journal of gastroenterology. 2003;9(10):2137.
• 13. Hoffmann PR, Berry MJ. The influence of selenium on immune responses. Molecular nutrition & food research. 2008;52(11):1273-80.
• 14. Gromer S, Eubel J, Lee B, Jacob J. Human selenoproteins at a glance. Cellular and Molecular Life Sciences CMLS. 2005;62(21):2414-37.
• 15. Kao C-Y, Sheu B-S, Wu J-J. Helicobacter pylori infection: An overview of bacterial virulence factors and pathogenesis. Biomedical journal. 2016;39(1):14-23.
• 16. Sayar R, Shokri Shirvani J, Hajian K, Vosough Z, Ranaei M. The negative association between inflammatory bowel disease and Helicobacter pylori seropositivity. Caspian Journal of Internal Medicine. 2019;10(2):217-22.
• 17. Polk DB, Peek Jr RM. Helicobacter pylori: gastric cancer and beyond. Nature Reviews Cancer. 2010;10(6):403.
• 18. Handa O, Naito Y, Yoshikawa T. Redox biology and gastric carcinogenesis: the role of Helicobacter pylori. Redox Report. 2011;16(1):1-7.
• 19. Nagata K, Yu H, Nishikawa M, Kashiba M, Nakamura A, Sato EF, et al. Helicobacter pylori generates superoxide radicals and modulates nitric oxide metabolism. Journal of Biological Chemistry. 1998;273(23):14071-3.
• 20. Mori M, Suzuki H, Suzuki M, Kai A, Miura S, Ishii H. Catalase and superoxide dismutase secreted from Helicobacter pylori. Helicobacter. 1997;2(2):100-5.
• 21. Götz J, Thio J, Verspaget H, Offerhaus G, Biemond I, Lamers C, et al. Treatment of Helicobacter pylori infection favourably affects gastric mucosal superoxide dismutases. Gut. 1997;40(5):591-6.
• 22. Stähler FN, Odenbreit S, Haas R, Wilrich J, Van Vliet AH, Kusters JG, et al. The novel Helicobacter pylori CznABC metal efflux pump is required for cadmium, zinc, and nickel resistance, urease modulation, and gastric colonization. Infection and immunity. 2006;74(7):3845-52.
• 23. Ge Z, Taylor DE. Helicobacter pylori genes hpcopA and hpcopP constitute a cop operon involved in copper export. FEMS microbiology letters. 1996;145(2):181-8.
• 24. Montefusco S, Esposito R, D’Andrea L, Monti MC, Dunne C, Dolan B, et al. Copper promotes TFF1-mediated Helicobacter pylori colonization. PLoS One. 2013;8(11):e79455.
• 25. Üstündag Y, Boyacioglu S, Haberal A, Demirhan B, Bilezikçi B. Plasma and gastric tissue selenium levels in patients with Helicobacter pylori infection. Journal of clinical gastroenterology. 2001;32(5):405-8.
• 26. Nezhad ZH, Moghaddam SD, Zahedi MJ, Hayatbakhsh M, Sharififar F, Meimand FE, et al. Serum selenium level in patients with gastric non-cardia cancer and functional dyspepsia. Iranian journal of medical sciences. 2015;40(3):214.
• 27. Cai X, Wang C, Yu W, Fan W, Wang S, Shen N, et al. Selenium exposure and cancer risk: an updated meta-analysis and meta-regression. Scientific reports. 2016;6:19213.
• 28. Wu M-C, Huang C-Y, Kuo F-C, Hsu W-H, Wang SS, Shih H-Y, et al. The effect of Helicobacter pylori eradication on the levels of essential trace elements. BioMed research international. 2014;2014.
• 29. Ugwuja EI, Akubugwo EI. Impact of Maternal Helicobacter pylori Infection on Trace Elements (Copper, Iron and Zinc) and Pregnancy Outcomes. Online Journal of Health and Allied Sciences. 2010;8(4).
• 30. Ozturk N, Kurt N, Ozgeris F, Baygutalp N, Tosun M, Bakan N. Serum zinc, copper, magnesium and selenium levels in children with Helicobacter pylori. Infection Eurasian J Med. 2015;47:126-9.
• 31. Hu A, Li L, Hu C, Zhang D, Wang C, Jiang Y, et al. Serum Concentrations of 15 Elements Among Helicobacter Pylori-Infected Residents from Lujiang County with High Gastric Cancer Risk in Eastern China. Biological trace element research. 2018:1-10.
• 32. Hacibekiroglu T, Basturk A, Akinci S, Bakanay S, Ulas T, Guney T, et al. Evaluation of serum levels of zinc, copper, and Helicobacter pylori IgG and IgA in iron deficiency anemia cases. European review for medical and pharmacological sciences. 2015;19(24):4835-40.
• 33. Janjetic MA, Goldman CG, Balcarce NE, Rua EC, González AB, Fuda JA, et al. Iron, zinc, and copper nutritional status in children infected with Helicobacter pylori. Journal of pediatric gastroenterology and nutrition. 2010;51(1):85-9.