Possible effects of some anaesthetic agents in rat hepatotoxicity: Histological and biochemical study

Year 2022, Volume: 39 Issue: 1, 84 - 90, 01.01.2022

Ebru Annaç , Öznur Uludag , Zümrüt Doğan

Abstract

Selectively steroidal neuromuscular blocking agents, such as rocuronium, are required to maintain surgical procedures. It is a sugammadex γ-cyclodextrin-derived drug that encapsulates these drugs and reverses its effects. In the present study, it was aimed to investigate the effects of sugammadex rocuronium complex on liver and possible toxic effects on hepatocytes using histopathological and biochemical methods. In the current study, 32 adult Sprague - Dawley male rats were used. Four groups were designed as pure control, control group, sugammadex and sugammadex-rocunorium. Following the experimental procedures, liver tissues extracted from rats were stained after routine histological procedures. The images were taken from the sections for histopathological evaluation. For biochemical analysis, glutathione (GSH) and malondialdehyde (MDA) enzymes were analyzed in the liver tissues. In the group treated with the sugammadex-rocunorium complex, caspase-3 expressions were observed to be higher than in other groups. In addition, while the amount of GSH belonging to sugammadex and sugammadex-rocunorium groups decreased compared to the control group, an increase in MDA amount was found. Sugammadex-rocunorium can lead to oxidative stress in the hepatocytes. However, more studies are needed to reveal the toxic effects of sugammadex and sugammadex and rocunorium complex in the liver.

Keywords

Sugammadex, Rocuronium, Oxidative stress, Immunohistochemistry, Liver histology, Rat

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