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Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats

Year 2022, Volume: 39 Issue: 2, 376 - 382, 18.03.2022

Abstract

Tropisetron, an antagonist of 5-HT3 receptor (5-HT3R), has exhibited a number of beneficial effects on the treatment of several diseases; however, its effect on diabetic cardiomyopathy, which is an important causal factor in morbidity and mortality among diabetic patients, remains to be fully elucidated. Therefore, this study was designed to investigate the effect of tropisetron on diabetes-induced cardiomyopathy and the molecular mediators possibly involved. Twenty-four male wistar rats were assigned into three groups, control, diabetic, and tropisetron–treated diabetic groups. After 14 days of treatment, the results revealed a significant increase in calcium/calmodulin-dependent protein kinaseIIδ (CaMKIIδ) total and isoforms δ2 and δ3 of CaMKIIδ, as well as myosin heavy chain (MHC)-β. Furthermore, it decreased MHC-α gene expression among the diabetic group compared to the control group. Western blot analysis showed a significant increase in Bax and cleaved caspase 3 protein levels and a significant decrease in Bcl-2 contents in heart tissue of diabetic rats in comparison to the control rats. Moreover, the levels of Tumor necrosis factor alpha (TNF-α) , ICAM-1, and CaMKII in heart tissue of diabetic rats were significantly higher than those in the control rats. Significant amelioration of alteration in the genes expression and protein changes along with restoration of the elevated levels of TNF-α, ICAM-1, and CaMKII were found in the tropisetron–treated diabetic group compared to the diabetic group. Collectively, our study provided strong evidence that 5-HT3 antagonist tropisetron was capable of attenuating the development of experimental diabetic cardiomyopathy associated with the reduction of intramyocardial MHCs and CaMKIIδ gene expression, inflammation and cardiac hypertrophy.

References

  • 1. Aminzadeh, A. 2017. Protective effect of tropisetron on high glucose induced apoptosis and oxidative stress in PC12 cells: roles of JNK, P38 MAPKs, and mitochondria pathway. Metab Brain Dis. 32: 819-826
  • 2. Anderson, M E ., Brown, J H ., Bers, D M. 2011. CaMKII in myocardial hypertrophy and heart failure. J Mol Cell Cardiol. 51: 468-473
  • 3. Becher, P M ., Lindner, D ., Frohlich, M ., Savvatis, K ., Westermann, D ., Tschope, C. 2013. Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: a time course analysis. Int J Mol Med. 32: 158-164
  • 4. Cheng, S M ., Ho, T J ., Yang, A L ., Chen, I J ., Kao, C L ., Wu, F N ., Lin, J A ., Kuo, C H ., Ou, H C ., Huang, C Y ., Lee, S D. 2013. Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways in streptozotocin-induced diabetic rats. Int J Cardiol. 167: 478-485
  • 5. Clerk, A ., Harrison, J G ., Long, C S ., Sugden, P H. 1999. Pro-inflammatory cytokines stimulate mitogen-activated protein kinase subfamilies, increase phosphorylation of c-Jun and ATF2 and upregulate c-Jun protein in neonatal rat ventricular myocytes. J Mol Cell Cardiol. 31: 2087-2099
  • 6. Communal, C ., Singh, K ., Pimentel, D R ., Colucci, W S. 1998. Norepinephrine stimulates apoptosis in adult rat ventricular myocytes by activation of the beta-adrenergic pathway. Circulation. 98: 1329-1334
  • 7. Daniels, L J ., Wallace, R S ., Nicholson, O M ., Wilson, G A ., McDonald, F J ., Jones, P P ., Baldi, J C ., Lamberts, R R ., Erickson, J R. 2018. Inhibition of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic rats. Cardiovasc Diabetol. 17: 89
  • 8. Dokken, B B. 2008. The Pathophysiology of Cardiovascular Disease and Diabetes: Beyond Blood Pressure and Lipids. Diabetes Spectrum. 21: 160-165
  • 9. Erickson, J R ., Joiner, M L ., Guan, X ., Kutschke, W ., Yang, J ., Oddis, C V ., Bartlett, R K ., Lowe, J S ., O'Donnell, S E ., Aykin-Burns, N ., Zimmerman, M C ., Zimmerman, K ., Ham, A J ., Weiss, R M ., Spitz, D R ., Shea, M A ., Colbran, R J ., Mohler, P J ., Anderson, M E. 2008. A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. Cell. 133: 462-474
  • 10. Fu, L W ., Longhurst, J C. 2002. Activated platelets contribute to stimulation of cardiac afferents during ischaemia in cats: role of 5-HT(3) receptors. J Physiol. 544: 897-912
  • 11. Grimm, M ., Ling, H ., Willeford, A ., Pereira, L ., Gray, C B ., Erickson, J R ., Sarma, S ., Respress, J L ., Wehrens, X H ., Bers, D M ., Brown, J H. 2015. CaMKIIdelta mediates beta-adrenergic effects on RyR2 phosphorylation and SR Ca(2+) leak and the pathophysiological response to chronic beta-adrenergic stimulation. J Mol Cell Cardiol. 85: 282-291
  • 12. Hegab, I I. 2018. Ameliorative effect of apelin on streptozotocin-induced diabetes and its associated cardiac hypertrophy. Alexandria Journal of Medicine. 54: 119-127
  • 13. Heshmati, E ., Shirpoor, A ., Kheradmand, F ., Alizadeh, M ., Gharalari, F H. 2018. Chronic ethanol increases calcium/calmodulin-dependent protein kinaseIIdelta gene expression and decreases monoamine oxidase amount in rat heart muscles: Rescue effect of Zingiber officinale (ginger) extract. Anatol J Cardiol. 19: 19-26
  • 14. Hoch, B ., Meyer, R ., Hetzer, R ., Krause, E G ., Karczewski, P. 1999. Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium. Circ Res. 84: 713-721
  • 15. Hochhauser, E ., Kivity, S ., Offen, D ., Maulik, N ., Otani, H ., Barhum, Y ., Pannet, H ., Shneyvays, V ., Shainberg, A ., Goldshtaub, V ., Tobar, A ., Vidne, B A. 2003. Bax ablation protects against myocardial ischemia-reperfusion injury in transgenic mice. Am J Physiol Heart Circ Physiol. 284: H2351-2359
  • 16. Huo, R ., Chen, C ., Chen, Y ., Li, Z ., Hou, Y ., Dong, D. 2012. 5-HT3 receptor antagonists protect against pressure overload-induced cardiac hypertrophy in murine. Acta Pharmaceutica Sinica B. 2: 16-22
  • 17. Izumo, S ., Lompre, A M ., Matsuoka, R ., Koren, G ., Schwartz, K ., Nadal-Ginard, B ., Mahdavi, V. 1987. Myosin heavy chain messenger RNA and protein isoform transitions during cardiac hypertrophy. Interaction between hemodynamic and thyroid hormone-induced signals. J Clin Invest. 79: 970-977
  • 18. Kayama, Y ., Raaz, U ., Jagger, A ., Adam, M ., Schellinger, I N ., Sakamoto, M ., Suzuki, H ., Toyama, K ., Spin, J M ., Tsao, P S. 2015. Diabetic Cardiovascular Disease Induced by Oxidative Stress. Int J Mol Sci. 16: 25234-25263
  • 19. Kelly, R A ., Smith, T W. 1997. Cytokines and cardiac contractile function. Circulation. 95: 778-781 20. Kroemer, G ., Dallaporta, B ., Resche-Rigon, M. 1998. The mitochondrial death/life regulator in apoptosis and necrosis. Annu Rev Physiol. 60: 619-642
  • 21. Lee, T I ., Kao, Y H ., Chen, Y C ., Huang, J H ., Hsiao, F C ., Chen, Y J. 2013. Peroxisome proliferator-activated receptors modulate cardiac dysfunction in diabetic cardiomyopathy. Diabetes Res Clin Pract. 100: 330-339
  • 22. Ling, H ., Gray, C B ., Zambon, A C ., Grimm, M ., Gu, Y ., Dalton, N ., Purcell, N H ., Peterson, K ., Brown, J H. 2013. Ca2+/Calmodulin-dependent protein kinase II delta mediates myocardial ischemia/reperfusion injury through nuclear factor-kappaB. Circ Res. 112: 935-944
  • 23. Longhurst, J C ., Tjen, A L S C ., Fu, L W. 2001. Cardiac sympathetic afferent activation provoked by myocardial ischemia and reperfusion. Mechanisms and reflexes. Ann N Y Acad Sci. 940: 74-95
  • 24. Luo, M ., Guan, X ., Luczak, E D ., Lang, D ., Kutschke, W ., Gao, Z ., Yang, J ., Glynn, P ., Sossalla, S ., Swaminathan, P D ., Weiss, R M ., Yang, B ., Rokita, A G ., Maier, L S ., Efimov, I R ., Hund, T J ., Anderson, M E. 2013. Diabetes increases mortality after myocardial infarction by oxidizing CaMKII. J Clin Invest. 123: 1262-1274
  • 25. Maier, L S. 2005. CaMKIIdelta overexpression in hypertrophy and heart failure: cellular consequences for excitation-contraction coupling. Braz J Med Biol Res. 38: 1293-1302
  • 26. Meehan, A G ., Kreulen, D L. 1991. Electrophysiological studies on the interaction of 5-hydroxytryptamine with sympathetic transmission in the guinea pig inferior mesenteric artery and ganglion. J Pharmacol Exp Ther. 256: 82-87
  • 27. Monasterolo, L A. 2014. Strategies in diabetic nephropathy: apelin is making its way. J Physiol. 592: 423-424
  • 28. Mongeau, R ., De Montigny, C ., Blier, P. 1994. Activation of 5-HT3 receptors enhances the electrically evoked release of [3H]noradrenaline in rat brain limbic structures. Eur J Pharmacol. 256: 269-279
  • 29. Palmieri, V ., Capaldo, B ., Russo, C ., Iaccarino, M ., Pezzullo, S ., Quintavalle, G ., Di Minno, G ., Riccardi, G ., Celentano, A. 2008. Uncomplicated type 1 diabetes and preclinical left ventricular myocardial dysfunction: insights from echocardiography and exercise cardiac performance evaluation. Diabetes Res Clin Pract. 79: 262-268
  • 30. Rajesh, M ., Mukhopadhyay, P ., Batkai, S ., Patel, V ., Saito, K ., Matsumoto, S ., Kashiwaya, Y ., Horvath, B ., Mukhopadhyay, B ., Becker, L ., Hasko, G ., Liaudet, L ., Wink, D A ., Veves, A ., Mechoulam, R ., Pacher, P. 2010. Cannabidiol attenuates cardiac dysfunction, oxidative stress, fibrosis, and inflammatory and cell death signaling pathways in diabetic cardiomyopathy. J Am Coll Cardiol. 56: 2115-2125
  • 31. Sack, M N. 2009. Type 2 diabetes, mitochondrial biology and the heart. J Mol Cell Cardiol. 46: 842-849
  • 32. Shao, C H ., Rozanski, G J ., Nagai, R ., Stockdale, F E ., Patel, K P ., Wang, M ., Singh, J ., Mayhan, W G ., Bidasee, K R. 2010. Carbonylation of myosin heavy chains in rat heart during diabetes. Biochem Pharmacol. 80: 205-217
  • 33. Shao, C H ., Rozanski, G J ., Patel, K P ., Bidasee, K R. 2007. Dyssynchronous (non-uniform) Ca2+ release in myocytes from streptozotocin-induced diabetic rats. J Mol Cell Cardiol. 42: 234-246
  • 34. Shirpoor, A. 2017. Medicinal plants for management of diabetes: alternative or adjuvant? Anatol J Cardiol. 17: 460
  • 35. Shirpoor, A ., Heshmatian, B ., Tofighi, A ., Eliasabad, S N ., Kheradmand, F ., Zerehpoosh, M. 2019. Nandrolone administration with or without strenuous exercise increases cardiac fatal genes overexpression, calcium/calmodulin-dependent protein kinaseiidelta, and monoamine oxidase activities and enhances blood pressure in adult wistar rats. Gene. 697: 131-137
  • 36. Shirpoor, A ., Ilkhanizadeh, B ., Saadatian, R ., Darvari, B S ., Behtaj, F ., Karimipour, M ., Ghaderi-Pakdel, F ., Saboori, E. 2006. Effect of vitamin E on diabetes-induced changes in small intestine and plasma antioxidant capacity in rat. J Physiol Biochem. 62: 171-177
  • 37. Shirpoor, A ., Khadem Ansari, M H ., Heshmatian, B ., Ilkhanizadeh, B ., Noruzi, L ., Abdollahzadeh, N ., Saboory, E. 2012. Decreased blood pressure with a corresponding decrease in adhesive molecules in diabetic rats caused by vitamin E administration. J Diabetes. 4: 362-368
  • 38. Shirpoor, A ., Zerehpoosh, M ., Ansari, M H K ., Kheradmand, F ., Rasmi, Y. 2017. Ginger extract mitigates ethanol-induced changes of alpha and beta – myosin heavy chain isoforms gene expression and oxidative stress in the heart of male wistar rats. DNA Repair. 57: 45-49
  • 39. Tak, P P ., Firestein, G S. 2001. NF-kappaB: a key role in inflammatory diseases. J Clin Invest. 107: 7-11
  • 40. Wang, W ., Ma, R. 2000. Cardiac sympathetic afferent reflexes in heart failure. Heart Fail Rev. 5: 57-71
  • 41. Wang, X T ., Gong, Y ., Zhou, B ., Yang, J J ., Cheng, Y ., Zhao, J G ., Qi, M Y. 2018. Ursolic acid ameliorates oxidative stress, inflammation and fibrosis in diabetic cardiomyopathy rats. Biomed Pharmacother. 97: 1461-1467
  • 42. Weiss, A ., Leinwand, L A. 1996. The mammalian myosin heavy chain gene family. Annu Rev Cell Dev Biol. 12: 417-439
  • 43. Westermann, D ., Van Linthout, S ., Dhayat, S ., Dhayat, N ., Schmidt, A ., Noutsias, M ., Song, X Y ., Spillmann, F ., Riad, A ., Schultheiss, H P ., Tschope, C. 2007. Tumor necrosis factor-alpha antagonism protects from myocardial inflammation and fibrosis in experimental diabetic cardiomyopathy. Basic Res Cardiol. 102: 500-507
  • 44. Wright, J W ., Mizutani, S ., Harding, J W. 2008. Pathways involved in the transition from hypertension to hypertrophy to heart failure. Treatment strategies. Heart Fail Rev. 13: 367-375
  • 45. Zhang, G X ., Kimura, S ., Nishiyama, A ., Shokoji, T ., Rahman, M ., Yao, L ., Nagai, Y ., Fujisawa, Y ., Miyatake, A ., Abe, Y. 2005. Cardiac oxidative stress in acute and chronic isoproterenol-infused rats. Cardiovasc Res. 65: 230-238
  • 46. Zhang, T ., Maier, L S ., Dalton, N D ., Miyamoto, S ., Ross, J, Jr. ., Bers, D M ., Brown, J H. 2003. The deltaC isoform of CaMKII is activated in cardiac hypertrophy and induces dilated cardiomyopathy and heart failure. Circ Res. 92: 912-919
Year 2022, Volume: 39 Issue: 2, 376 - 382, 18.03.2022

Abstract

References

  • 1. Aminzadeh, A. 2017. Protective effect of tropisetron on high glucose induced apoptosis and oxidative stress in PC12 cells: roles of JNK, P38 MAPKs, and mitochondria pathway. Metab Brain Dis. 32: 819-826
  • 2. Anderson, M E ., Brown, J H ., Bers, D M. 2011. CaMKII in myocardial hypertrophy and heart failure. J Mol Cell Cardiol. 51: 468-473
  • 3. Becher, P M ., Lindner, D ., Frohlich, M ., Savvatis, K ., Westermann, D ., Tschope, C. 2013. Assessment of cardiac inflammation and remodeling during the development of streptozotocin-induced diabetic cardiomyopathy in vivo: a time course analysis. Int J Mol Med. 32: 158-164
  • 4. Cheng, S M ., Ho, T J ., Yang, A L ., Chen, I J ., Kao, C L ., Wu, F N ., Lin, J A ., Kuo, C H ., Ou, H C ., Huang, C Y ., Lee, S D. 2013. Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways in streptozotocin-induced diabetic rats. Int J Cardiol. 167: 478-485
  • 5. Clerk, A ., Harrison, J G ., Long, C S ., Sugden, P H. 1999. Pro-inflammatory cytokines stimulate mitogen-activated protein kinase subfamilies, increase phosphorylation of c-Jun and ATF2 and upregulate c-Jun protein in neonatal rat ventricular myocytes. J Mol Cell Cardiol. 31: 2087-2099
  • 6. Communal, C ., Singh, K ., Pimentel, D R ., Colucci, W S. 1998. Norepinephrine stimulates apoptosis in adult rat ventricular myocytes by activation of the beta-adrenergic pathway. Circulation. 98: 1329-1334
  • 7. Daniels, L J ., Wallace, R S ., Nicholson, O M ., Wilson, G A ., McDonald, F J ., Jones, P P ., Baldi, J C ., Lamberts, R R ., Erickson, J R. 2018. Inhibition of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic rats. Cardiovasc Diabetol. 17: 89
  • 8. Dokken, B B. 2008. The Pathophysiology of Cardiovascular Disease and Diabetes: Beyond Blood Pressure and Lipids. Diabetes Spectrum. 21: 160-165
  • 9. Erickson, J R ., Joiner, M L ., Guan, X ., Kutschke, W ., Yang, J ., Oddis, C V ., Bartlett, R K ., Lowe, J S ., O'Donnell, S E ., Aykin-Burns, N ., Zimmerman, M C ., Zimmerman, K ., Ham, A J ., Weiss, R M ., Spitz, D R ., Shea, M A ., Colbran, R J ., Mohler, P J ., Anderson, M E. 2008. A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. Cell. 133: 462-474
  • 10. Fu, L W ., Longhurst, J C. 2002. Activated platelets contribute to stimulation of cardiac afferents during ischaemia in cats: role of 5-HT(3) receptors. J Physiol. 544: 897-912
  • 11. Grimm, M ., Ling, H ., Willeford, A ., Pereira, L ., Gray, C B ., Erickson, J R ., Sarma, S ., Respress, J L ., Wehrens, X H ., Bers, D M ., Brown, J H. 2015. CaMKIIdelta mediates beta-adrenergic effects on RyR2 phosphorylation and SR Ca(2+) leak and the pathophysiological response to chronic beta-adrenergic stimulation. J Mol Cell Cardiol. 85: 282-291
  • 12. Hegab, I I. 2018. Ameliorative effect of apelin on streptozotocin-induced diabetes and its associated cardiac hypertrophy. Alexandria Journal of Medicine. 54: 119-127
  • 13. Heshmati, E ., Shirpoor, A ., Kheradmand, F ., Alizadeh, M ., Gharalari, F H. 2018. Chronic ethanol increases calcium/calmodulin-dependent protein kinaseIIdelta gene expression and decreases monoamine oxidase amount in rat heart muscles: Rescue effect of Zingiber officinale (ginger) extract. Anatol J Cardiol. 19: 19-26
  • 14. Hoch, B ., Meyer, R ., Hetzer, R ., Krause, E G ., Karczewski, P. 1999. Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium. Circ Res. 84: 713-721
  • 15. Hochhauser, E ., Kivity, S ., Offen, D ., Maulik, N ., Otani, H ., Barhum, Y ., Pannet, H ., Shneyvays, V ., Shainberg, A ., Goldshtaub, V ., Tobar, A ., Vidne, B A. 2003. Bax ablation protects against myocardial ischemia-reperfusion injury in transgenic mice. Am J Physiol Heart Circ Physiol. 284: H2351-2359
  • 16. Huo, R ., Chen, C ., Chen, Y ., Li, Z ., Hou, Y ., Dong, D. 2012. 5-HT3 receptor antagonists protect against pressure overload-induced cardiac hypertrophy in murine. Acta Pharmaceutica Sinica B. 2: 16-22
  • 17. Izumo, S ., Lompre, A M ., Matsuoka, R ., Koren, G ., Schwartz, K ., Nadal-Ginard, B ., Mahdavi, V. 1987. Myosin heavy chain messenger RNA and protein isoform transitions during cardiac hypertrophy. Interaction between hemodynamic and thyroid hormone-induced signals. J Clin Invest. 79: 970-977
  • 18. Kayama, Y ., Raaz, U ., Jagger, A ., Adam, M ., Schellinger, I N ., Sakamoto, M ., Suzuki, H ., Toyama, K ., Spin, J M ., Tsao, P S. 2015. Diabetic Cardiovascular Disease Induced by Oxidative Stress. Int J Mol Sci. 16: 25234-25263
  • 19. Kelly, R A ., Smith, T W. 1997. Cytokines and cardiac contractile function. Circulation. 95: 778-781 20. Kroemer, G ., Dallaporta, B ., Resche-Rigon, M. 1998. The mitochondrial death/life regulator in apoptosis and necrosis. Annu Rev Physiol. 60: 619-642
  • 21. Lee, T I ., Kao, Y H ., Chen, Y C ., Huang, J H ., Hsiao, F C ., Chen, Y J. 2013. Peroxisome proliferator-activated receptors modulate cardiac dysfunction in diabetic cardiomyopathy. Diabetes Res Clin Pract. 100: 330-339
  • 22. Ling, H ., Gray, C B ., Zambon, A C ., Grimm, M ., Gu, Y ., Dalton, N ., Purcell, N H ., Peterson, K ., Brown, J H. 2013. Ca2+/Calmodulin-dependent protein kinase II delta mediates myocardial ischemia/reperfusion injury through nuclear factor-kappaB. Circ Res. 112: 935-944
  • 23. Longhurst, J C ., Tjen, A L S C ., Fu, L W. 2001. Cardiac sympathetic afferent activation provoked by myocardial ischemia and reperfusion. Mechanisms and reflexes. Ann N Y Acad Sci. 940: 74-95
  • 24. Luo, M ., Guan, X ., Luczak, E D ., Lang, D ., Kutschke, W ., Gao, Z ., Yang, J ., Glynn, P ., Sossalla, S ., Swaminathan, P D ., Weiss, R M ., Yang, B ., Rokita, A G ., Maier, L S ., Efimov, I R ., Hund, T J ., Anderson, M E. 2013. Diabetes increases mortality after myocardial infarction by oxidizing CaMKII. J Clin Invest. 123: 1262-1274
  • 25. Maier, L S. 2005. CaMKIIdelta overexpression in hypertrophy and heart failure: cellular consequences for excitation-contraction coupling. Braz J Med Biol Res. 38: 1293-1302
  • 26. Meehan, A G ., Kreulen, D L. 1991. Electrophysiological studies on the interaction of 5-hydroxytryptamine with sympathetic transmission in the guinea pig inferior mesenteric artery and ganglion. J Pharmacol Exp Ther. 256: 82-87
  • 27. Monasterolo, L A. 2014. Strategies in diabetic nephropathy: apelin is making its way. J Physiol. 592: 423-424
  • 28. Mongeau, R ., De Montigny, C ., Blier, P. 1994. Activation of 5-HT3 receptors enhances the electrically evoked release of [3H]noradrenaline in rat brain limbic structures. Eur J Pharmacol. 256: 269-279
  • 29. Palmieri, V ., Capaldo, B ., Russo, C ., Iaccarino, M ., Pezzullo, S ., Quintavalle, G ., Di Minno, G ., Riccardi, G ., Celentano, A. 2008. Uncomplicated type 1 diabetes and preclinical left ventricular myocardial dysfunction: insights from echocardiography and exercise cardiac performance evaluation. Diabetes Res Clin Pract. 79: 262-268
  • 30. Rajesh, M ., Mukhopadhyay, P ., Batkai, S ., Patel, V ., Saito, K ., Matsumoto, S ., Kashiwaya, Y ., Horvath, B ., Mukhopadhyay, B ., Becker, L ., Hasko, G ., Liaudet, L ., Wink, D A ., Veves, A ., Mechoulam, R ., Pacher, P. 2010. Cannabidiol attenuates cardiac dysfunction, oxidative stress, fibrosis, and inflammatory and cell death signaling pathways in diabetic cardiomyopathy. J Am Coll Cardiol. 56: 2115-2125
  • 31. Sack, M N. 2009. Type 2 diabetes, mitochondrial biology and the heart. J Mol Cell Cardiol. 46: 842-849
  • 32. Shao, C H ., Rozanski, G J ., Nagai, R ., Stockdale, F E ., Patel, K P ., Wang, M ., Singh, J ., Mayhan, W G ., Bidasee, K R. 2010. Carbonylation of myosin heavy chains in rat heart during diabetes. Biochem Pharmacol. 80: 205-217
  • 33. Shao, C H ., Rozanski, G J ., Patel, K P ., Bidasee, K R. 2007. Dyssynchronous (non-uniform) Ca2+ release in myocytes from streptozotocin-induced diabetic rats. J Mol Cell Cardiol. 42: 234-246
  • 34. Shirpoor, A. 2017. Medicinal plants for management of diabetes: alternative or adjuvant? Anatol J Cardiol. 17: 460
  • 35. Shirpoor, A ., Heshmatian, B ., Tofighi, A ., Eliasabad, S N ., Kheradmand, F ., Zerehpoosh, M. 2019. Nandrolone administration with or without strenuous exercise increases cardiac fatal genes overexpression, calcium/calmodulin-dependent protein kinaseiidelta, and monoamine oxidase activities and enhances blood pressure in adult wistar rats. Gene. 697: 131-137
  • 36. Shirpoor, A ., Ilkhanizadeh, B ., Saadatian, R ., Darvari, B S ., Behtaj, F ., Karimipour, M ., Ghaderi-Pakdel, F ., Saboori, E. 2006. Effect of vitamin E on diabetes-induced changes in small intestine and plasma antioxidant capacity in rat. J Physiol Biochem. 62: 171-177
  • 37. Shirpoor, A ., Khadem Ansari, M H ., Heshmatian, B ., Ilkhanizadeh, B ., Noruzi, L ., Abdollahzadeh, N ., Saboory, E. 2012. Decreased blood pressure with a corresponding decrease in adhesive molecules in diabetic rats caused by vitamin E administration. J Diabetes. 4: 362-368
  • 38. Shirpoor, A ., Zerehpoosh, M ., Ansari, M H K ., Kheradmand, F ., Rasmi, Y. 2017. Ginger extract mitigates ethanol-induced changes of alpha and beta – myosin heavy chain isoforms gene expression and oxidative stress in the heart of male wistar rats. DNA Repair. 57: 45-49
  • 39. Tak, P P ., Firestein, G S. 2001. NF-kappaB: a key role in inflammatory diseases. J Clin Invest. 107: 7-11
  • 40. Wang, W ., Ma, R. 2000. Cardiac sympathetic afferent reflexes in heart failure. Heart Fail Rev. 5: 57-71
  • 41. Wang, X T ., Gong, Y ., Zhou, B ., Yang, J J ., Cheng, Y ., Zhao, J G ., Qi, M Y. 2018. Ursolic acid ameliorates oxidative stress, inflammation and fibrosis in diabetic cardiomyopathy rats. Biomed Pharmacother. 97: 1461-1467
  • 42. Weiss, A ., Leinwand, L A. 1996. The mammalian myosin heavy chain gene family. Annu Rev Cell Dev Biol. 12: 417-439
  • 43. Westermann, D ., Van Linthout, S ., Dhayat, S ., Dhayat, N ., Schmidt, A ., Noutsias, M ., Song, X Y ., Spillmann, F ., Riad, A ., Schultheiss, H P ., Tschope, C. 2007. Tumor necrosis factor-alpha antagonism protects from myocardial inflammation and fibrosis in experimental diabetic cardiomyopathy. Basic Res Cardiol. 102: 500-507
  • 44. Wright, J W ., Mizutani, S ., Harding, J W. 2008. Pathways involved in the transition from hypertension to hypertrophy to heart failure. Treatment strategies. Heart Fail Rev. 13: 367-375
  • 45. Zhang, G X ., Kimura, S ., Nishiyama, A ., Shokoji, T ., Rahman, M ., Yao, L ., Nagai, Y ., Fujisawa, Y ., Miyatake, A ., Abe, Y. 2005. Cardiac oxidative stress in acute and chronic isoproterenol-infused rats. Cardiovasc Res. 65: 230-238
  • 46. Zhang, T ., Maier, L S ., Dalton, N D ., Miyamoto, S ., Ross, J, Jr. ., Bers, D M ., Brown, J H. 2003. The deltaC isoform of CaMKII is activated in cardiac hypertrophy and induces dilated cardiomyopathy and heart failure. Circ Res. 92: 912-919
There are 45 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Clinical Research
Authors

Roya Naderi This is me 0000-0001-8529-7306

Alireza Shirpoor 0000-0002-3663-6823

Mahrokh Samadi This is me 0000-0001-6493-1304

Farideh Nezamimajd This is me 0000-0003-2564-5991

Yousef Rasmi This is me 0000-0003-1506-1909

Morteza Bagheri This is me 0000-0002-4739-8517

Early Pub Date March 18, 2022
Publication Date March 18, 2022
Submission Date August 20, 2021
Acceptance Date January 31, 2022
Published in Issue Year 2022 Volume: 39 Issue: 2

Cite

APA Naderi, R., Shirpoor, A., Samadi, M., Nezamimajd, F., et al. (2022). Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats. Journal of Experimental and Clinical Medicine, 39(2), 376-382.
AMA Naderi R, Shirpoor A, Samadi M, Nezamimajd F, Rasmi Y, Bagheri M. Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats. J. Exp. Clin. Med. March 2022;39(2):376-382.
Chicago Naderi, Roya, Alireza Shirpoor, Mahrokh Samadi, Farideh Nezamimajd, Yousef Rasmi, and Morteza Bagheri. “Protective Effects of Tropisetron on Diabetes-Induced Expression of Genes Involved in Regulation of Contractility, Cardiomyopathy and Apoptosis in the Left Ventricle of Rats”. Journal of Experimental and Clinical Medicine 39, no. 2 (March 2022): 376-82.
EndNote Naderi R, Shirpoor A, Samadi M, Nezamimajd F, Rasmi Y, Bagheri M (March 1, 2022) Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats. Journal of Experimental and Clinical Medicine 39 2 376–382.
IEEE R. Naderi, A. Shirpoor, M. Samadi, F. Nezamimajd, Y. Rasmi, and M. Bagheri, “Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats”, J. Exp. Clin. Med., vol. 39, no. 2, pp. 376–382, 2022.
ISNAD Naderi, Roya et al. “Protective Effects of Tropisetron on Diabetes-Induced Expression of Genes Involved in Regulation of Contractility, Cardiomyopathy and Apoptosis in the Left Ventricle of Rats”. Journal of Experimental and Clinical Medicine 39/2 (March 2022), 376-382.
JAMA Naderi R, Shirpoor A, Samadi M, Nezamimajd F, Rasmi Y, Bagheri M. Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats. J. Exp. Clin. Med. 2022;39:376–382.
MLA Naderi, Roya et al. “Protective Effects of Tropisetron on Diabetes-Induced Expression of Genes Involved in Regulation of Contractility, Cardiomyopathy and Apoptosis in the Left Ventricle of Rats”. Journal of Experimental and Clinical Medicine, vol. 39, no. 2, 2022, pp. 376-82.
Vancouver Naderi R, Shirpoor A, Samadi M, Nezamimajd F, Rasmi Y, Bagheri M. Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats. J. Exp. Clin. Med. 2022;39(2):376-82.