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Mammalian cell lines used in Bioprocessing

Year 2022, Volume: 39 Issue: 3, 884 - 892, 30.08.2022

Abstract

Various number of expression and host systems are used in biologics manufacturing. The most commonly preferred systems are based on bacteria, yeast, mammalian cells, insect cells, and transgenic animals. A wide range of molecules including insulin, mAbs, vaccines and recombinant proteins are produced by using different kind of host systems. Because of several reasons impacting the product quality and yield, mammalian cells are utilized. Moreover, mammalian cells are generally used in virus based vaccine manufacturing. Chinese Hamster Ovary (CHO) is the most widely used cell line for high yield stable recombinant protein production, while Human Embronic Kidney (HEK) is favoured for transient transfection low yield protein manufacturing, viral based vaccine and gene and cell therapy related vector production. Other mammalian cell lines such as NSO, Sp2.0, Vero, MRC-5 and PerC.6 are also used in both recombinant protein and virus productions. Multiple modifications are carried out on industrial cell lines in order to make them more suitable both for high yield and high quality protein production. Thanks to all of these alterations, high productivity and quality levels are achieved in biotechnology industry.

References

  • 1 EvaluatePharma World Preview 2019, Outlook to 2024. [online] Available at: <https://www.evaluate.com/thought-leadership/pharma/evaluatepharma-world-preview-2019-outlook-2024> . 2 Tripathi, N. and Shrivastava, A., 2019. Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development. Frontiers in Bioengineering and Biotechnology, 7.
Year 2022, Volume: 39 Issue: 3, 884 - 892, 30.08.2022

Abstract

References

  • 1 EvaluatePharma World Preview 2019, Outlook to 2024. [online] Available at: <https://www.evaluate.com/thought-leadership/pharma/evaluatepharma-world-preview-2019-outlook-2024> . 2 Tripathi, N. and Shrivastava, A., 2019. Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development. Frontiers in Bioengineering and Biotechnology, 7.
There are 1 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Review Articles
Authors

Nuri Ergen 0000-0002-3673-8520

Halil Tüfekçi 0000-0001-5323-9929

Early Pub Date August 30, 2022
Publication Date August 30, 2022
Submission Date April 21, 2022
Acceptance Date June 5, 2022
Published in Issue Year 2022 Volume: 39 Issue: 3

Cite

APA Ergen, N., & Tüfekçi, H. (2022). Mammalian cell lines used in Bioprocessing. Journal of Experimental and Clinical Medicine, 39(3), 884-892.
AMA Ergen N, Tüfekçi H. Mammalian cell lines used in Bioprocessing. J. Exp. Clin. Med. August 2022;39(3):884-892.
Chicago Ergen, Nuri, and Halil Tüfekçi. “Mammalian Cell Lines Used in Bioprocessing”. Journal of Experimental and Clinical Medicine 39, no. 3 (August 2022): 884-92.
EndNote Ergen N, Tüfekçi H (August 1, 2022) Mammalian cell lines used in Bioprocessing. Journal of Experimental and Clinical Medicine 39 3 884–892.
IEEE N. Ergen and H. Tüfekçi, “Mammalian cell lines used in Bioprocessing”, J. Exp. Clin. Med., vol. 39, no. 3, pp. 884–892, 2022.
ISNAD Ergen, Nuri - Tüfekçi, Halil. “Mammalian Cell Lines Used in Bioprocessing”. Journal of Experimental and Clinical Medicine 39/3 (August 2022), 884-892.
JAMA Ergen N, Tüfekçi H. Mammalian cell lines used in Bioprocessing. J. Exp. Clin. Med. 2022;39:884–892.
MLA Ergen, Nuri and Halil Tüfekçi. “Mammalian Cell Lines Used in Bioprocessing”. Journal of Experimental and Clinical Medicine, vol. 39, no. 3, 2022, pp. 884-92.
Vancouver Ergen N, Tüfekçi H. Mammalian cell lines used in Bioprocessing. J. Exp. Clin. Med. 2022;39(3):884-92.