Investigation of Some Biological Activities of Inula graveolens (L.) Desf Species from Turkey

Year 2023, , 110 - 119, 31.12.2023

Ramazan Mammadov , Bayram Kaya , İlayda Cansu Atıcı , Mehmet Özgür Atay

https://doi.org/10.54370/ordubtd.1240934

Abstract

In this study, it was aimed to determine the phenolic and flavonoid content and different biological activities (antioxidant, enzyme inhibitor, anthelmintic) of the methanol extract of Inula graveolens (L.) Desf collected from Muğla (Turkey). As a result of the study, the total phenolic content was determined as 5.36±0.32 mg GAE/g, and the total flavonoid amount was determined as 3.49±0.05 mg QE/g extract equivalent. In the ß-carotene/linoleic acid method, the extract showed lower activity than the standard BHA used. The extract was determined to be equivalent to 4.28±0.24/0.47±0.03 mg TE/g extract in terms of copper and iron-reducing power capacity, respectively. Although the enzyme inhibitory activities of the extract increased with the increase in concentration, it was determined that it had lower activity than galantamine (89.41±0.05%) and kojic acid (73.93±0.10%) used as standard. Paralysis and death times of the extract at different concentrations (2.5,5,10,20 mg/mL) on Tubifex tubifex worms were determined. It was determined that the extract at high concentrations (20 mg/mL) exhibited an activity near that of andazole (10 mg/mL) used as a standard. According to these results, I. graveolens can be considered a good resource for the pharmaceutical industry due to its activities.

Keywords

Inula graveolens, antioxidant, enzyme inhibitor, Tubifex tubifex

Supporting Institution

TUBİTAK

Project Number

1919B012102904

Türkiye’den Toplanan Inula graveolens (L.) Desf. Türünün Bazı Biyolojik Aktivitelerinin Araştırılması

Abstract

Bu çalışmada Muğla'dan toplanan Inula graveolens (L.) Desf türünün metanol ekstraktının fenolik ve flavonoid miktarı ve farklı biyolojik aktivitelerinin (antioksidan, enzim inhibitör, antihelmint) belirlenmesi amaçlanmıştır. Çalışma sonucunda total fenolik miktarı 5,36±0,32 mg GAE/g, total flavonoid miktarı ise 3,49±0,05 mg QE/g ekstrakt eşdeğeri olarak belirlenmiştir. ß-karoten/linoleik asit yönteminde ekstrakt standart olarak kullanılan BHA’ya göre daha düşük bir aktivite sergilemiştir. Ekstraktın bakır ve demir indirgeme gücü kapasitesi bakımından sırasıyla 4,28±0,24/0,47±0,03 mg TE/g ekstrakt eşdeğeri olduğu belirlenmiştir. Ekstraktın enzim inhibitör aktiviteleri konsantrasyon artışına bağlı artsa da standart olarak kullanılan galantamin (89.41±0.05%) ve kojik asite (73.93±0.10%) göre daha düşük bir aktiviteye sahip olduğu tespit edilmiştir. Farklı konsantrasyondaki ekstraktın (2.5,5,10,20 mg/mL) Tubifex tubifex solucanları üzerindeki paralize ve ölüm süreleri belirlenmiştir. Yüksek konsantrasyonlardaki ekstraktın (20 mg/mL) standart olarak kullanılan andazole (10 mg/mL) yakın bir aktivite sergilediği belirlenmiştir. Bu sonuçlara göre I. graveolens gösterdiği aktivitelerden dolayı ilaç endüstrisi için iyi bir kaynak olarak dikkate alınabilir.

Keywords

Inula graveolens, Tubifex tubifex, antioksidan, enzim inhibitör

Project Number

1919B012102904

References

• Abdioğlu, D. M. (2019). Bazı meşe gallerinin kolinesteraz, tirozinaz ve üreaz enzim inhibisyonu ile antioksidan aktivitesinin belirlenmesi [Master’s thesis]. Batman University.
• Albayrak, S., Korkmaz-Çınar, A. E., Paksoy, M. Y., & Aksoy, A. (2015). An investigation on antioxidant and antimicrobial activities of four Inula helenium L. taxa. Iranian Journal of Science and Technology (Sciences), 39(4), 473-483. http://doi.org/10.22099/IJSTS.2015.3398
• Amarowicz, R., Pegg, R. B., Rahimi-Moghaddam, P., Barl, B., & Weil, J. A. (2004). Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry, 84(4), 551-562. http://doi.org/10.1016/S0308-8146(03)00278-4
• Apak, R., Güçlü, K., Özyürek, M. & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52(26), 7970-7981. https://doi.org/10.1021/jf048741x
• Aryal, B., & Suárez, Y. (2019). Non-coding RNA regulation of endothelial and macrophage functions during atherosclerosis. Vascular Pharmacology, 114, 64-75. https://doi.org/10.1016/j.vph.2018.03.001
• Asraoui, F., Kounnoun, A., Cacciola, F., El Mansouri, F., Kabach, I., Oulad El Majdoub, Y., ... & Louajri, A. (2021). Phytochemical profile, antioxidant capacity, α-amylase, and α-glucosidase inhibitory potential of wild Moroccan inula viscosa (L.) Aiton leaves. Molecules, 26(11), 3134. https://doi.org/10.3390/molecules26113134
• Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1996.0292
• Brahmi-Chendouh, N., Piccolella, S., Crescente, G., Pacifico, F., Boulekbache, L., Hamri-Zeghichi, S., ... & Pacifico, S. (2019). A nutraceutical extract from Inula viscosa leaves: UHPLC-HR-MS/MS-based polyphenol profile and antioxidant and cytotoxic activities. Journal of food and drug analysis, 27(3), 692-702. https://doi.org/10.1016/j.jfda.2018.11.006
• Bucchini, A., Ricci, D., Messina, F., Marcotullio, M. C., Curini, M., & Giamperi, L. (2015). Antioxidant and antifungal activity of different extracts obtained from aerial parts of Inula crithmoides L. Natural Product Research, 29(12), 1173-1176. https://doi.org/10.1080/14786419.2014.983102
• Bursal, E., Yılmaz, M. A., Izol, E., Türkan, F., Atalar, M. N., Murahari, M., ... & Ahmad, M. (2021). Enzyme inhibitory function and phytochemical profile of Inula discoidea using in vitro and in silico methods. Biophysical Chemistry, 277, 106629. https://doi.org/10.1016/j.bpc.2021.106629
• Ceylan, R., Zengin, G., Mahomoodally, M. F., Sinan, K. I., Ak, G., Jugreet, S., ... & Yılmaz, M. A. (2021). Enzyme inhibition and antioxidant functionality of eleven Inula species based on chemical components and chemometric insights. Biochemical Systematics and Ecology, 95, 104225. https://doi.org/10.1016/j.bse.2021.104225
• Chahmi, N., Anissi, J., Jennan, S., Farah, A., Sendide, K., & El Hassouni, M. (2015). Antioxidant activities and total phenol content of Inula viscosa extract selected from three regions of Morocco. Asian Pacific Journal of Tropical Biomedicine, 5(3), 228-233. https://doi.org/10.1016/S2221-1691(15)30010-1
• Çölgeçen, Ş, H. (2015). Bitki sekonder metabolitlerinin biyoreaktörlerde üretilmesi. Türk Bilimsel Derlemeler Dergisi, 8(2), 09-29. https://dergipark.org.tr/en/pub/derleme/issue/35095/389337
• Das, S. S., Dey, M., & Ghosh, A. K. (2011). Determination of the anthelmintic activity of the leaf and bark extract of Tamarindus indica Linn. Indian journal of pharmaceutical sciences, 73(1), 104. https://doi.org/10.4103/0250-474X.89768
• Dash, G. K., Suresh, P., Kar, D. M., Ganpaty, S., & Panda, S. B. (2002). Evaluation of Evolvulus Alsinoides Linn for anthelmintic and antimicrobial activities. Journal of Natural Remedies, 2(2), 182–185. https://doi.org/10.18311/jnr/2002/146
• Dey, Y. N., & Ghosh, A. K., (2010). Evaluation of the anthelmintic activity of the methanolic extract of Amorphophallus paeoniifolius tuber. International Journal of Pharmaceutical Sciences and Research, 1(11), 117. https://www.cabdirect.org/cabdirect/abstract/20113340164
• Ekor, M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4, 177. https://doi.org/10.3389/fphar.2013.00177
• Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2), 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
• Gökbulut, A. Y., & Şarer, E. T. D. (2011). Türkiye’de yetişen bazı Inula L. Türleri üzerinde farmakognozik araştırmalar [Doctoral dissertation]. Ankara University.
• Gökbulut, A., Özhana, O., Satılmiş, B., Batçioğlu, K., Günal, S., & Şarer, E. (2013). Antioxidant and antimicrobial activities, and phenolic compounds of selected Inula species from Turkey. Natural Product Communications, 8(4), 475-478. https://doi.org/10.1177/1934578X1300800417
• Güçlü, G., Ergül, M., Uçar E., Eruygur, N., Ataş M., & Akpulat, H. A. (2022). Anticancer, antioxidant, antimicrobial, and enzyme inhibitory activities Of Inula Aucheriana. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(5), 946-954. https://doi.org/10.18016/ksutarimdoga.vi.985837
• Haque, M. A., Kamal, A. M., & Chowdhury, K. A. A. (2014). Phytochemical investigation and assessment of in vivo and in vitro pharmacological activities of blumea lacera (burm. f.) dc. World Journal of Pharmaceutical Research, 8(3), 120-130. https://wjpr.s3.ap-south-1.amazonaws.com/article_issue/1425125643.pdf
• Hossain, M. M., Kabir, M. S. H., Chowdhury, T. A., Hasanat, A., & Chakrabarty, N. (2015). Anthelmintic effects of different extracts of Hopea odorata leaves on Tubifex tubifex worm using in vitro method and their condensed tannin content. Journal of Pharmaceutical Research International, 8(3), 1-7. https://doi.org/10.9734/BJPR/2015/19064
• Jallali, I., Zaouali, Y., Missaoui, I., Smeoui, A., Abdelly, C., & Ksouri, R. (2014). Variability of antioxidant and antibacterial effects of essential oils and acetonic extracts of two edible halophytes: Crithmum maritimum L. and Inula crithmoїdes L. Food Chemistry, 145, 1031-1038. https://doi.org/10.1016/j.foodchem.2013.09.034
• Karabulut, H., & Gülay, M. Ş. (2016). Serbest radikaller. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 4(1), 50-59. https://dergipark.org.tr/en/pub/maeusabed/issue/24655/260783
• Karan, T., Yildiz, I., Aydin, A., & Erenler, R. (2018). Inhibition of various cancer cells proliferation of Bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Records of Natural Products, 12(3), 274-284. https://doi.org/10.25135/rnp.30.17.09.057
• Kheyar-Kraouche, N., da Silva, A. B., Serra, A. T., Bedjou, F., & Bronze, M. R. (2018). Characterization by liquid chromatography–mass spectrometry and antioxidant activity of an ethanolic extract of Inula viscosa leaves. Journal Of Pharmaceutical And Biomedical Analysis, 156, 297-306. https://doi.org/10.1016/j.jpba.2018.04.047
• Mohti, H., Taviano, M. F., Cacciola, F., Dugo, P., Mondello, L., Marino, A., ... & Miceli, N. (2020). Inula viscosa (L.) Aiton leaves and flower buds: Effect of extraction solvent/technique on their antioxidant ability, antimicrobial properties, and phenolic profile. Natural Product Research, 34(1), 46-52. https://doi.org/10.1080/14786419.2019.1569659
• Ozkan, E., Karakas, F. P., Yildirim, A. B. B., Tas, I., Eker, I., Yavuz, M. Z., & Turker, A. U. (2019). Promising medicinal plant Inula viscosa L.: Antiproliferative, antioxidant, antibacterial, and phenolic profiles. Progress in Nutrition, 21(3), 652-661. https://doi.org/10.23751/pn.v21i3.7186
• Patil, B. S., Raut, I. D., Bhutkar, M. A., & Mohite, S. K. (2015). Evaluation of the anthelmintic activity of leaves of Tragia involucrata Linn. Journal of Pharmacognosy and Phytochemistry, 4(1), 155-159. https://www.phytojournal.com/archives/2015/vol4issue1/PartC/4-1-35.1-804.pdf
• Petrovska, B. B. (2012). Historical review of medicinal plants usage. Pharmacognosy Reviews, 6(11), 1-5. https://doi.org/10.4103/0973-7847.95849
• Sellem, I., Chakchouk-Mtibaa, A., Zaghden, H., Smaoui, S., Ennouri, K., & Mellouli, L. (2020). Harvesting season-dependent variation in chemical composition and biological activities of the essential oil obtained from Inula graveolens (L.) grown in Chebba (Tunisia) salt marsh. Arabian Journal of Chemistry, 13(3), 4835-4845. https://doi.org/10.1016/j.arabjc.2020.01.013
• Sharaf, O. Z., & Orhan, M. F. (2014). An overview of fuel cell technology: Fundamentals and application. Renewable and Sustainable Energy Reviews, 32, 810-853. https://doi.org/10.1016/j.rser.2014.01.012
• Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158. https://doi.org/10.5344/ajev.1965.16.3.144
• Tiring, G., Satar, S., & Özkaya, O. (2020). Sekonder metabolitler. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(1), 203-215. https://dergipark.org.tr/en/pub/bursauludagziraat/issue/58016/721701
• Trendafilova, A., Ivanova, V., Rangelov, M., Todorova, M., Ozek, G., Yur, S., ... & Topouzova‐Hristova, T. (2020). Caffeoylquinic acids, cytotoxic, antioxidant, acetylcholinesterase, and tyrosinase enzyme inhibitory activities of six Inula species from Bulgaria. Chemistry & Biodiversity, 17(4), e2000051. https://doi.org/10.1002/cbdv.202000051
• Turan, M., & Mammadov, R. (2018). Antioxidant, antimicrobial, cytotoxic, larvicidal, and anthelmintic activities and phenolic contents of Cyclamen alpinum. Pharmacology & Pharmacy, 9(4), 100-116. https://doi.org/10.4236/pp.2018.94008
• Yıldırım, A., Ali, Ş. E. N., Tuysuz, M., Tan, A. S. B., Şenkardeş, İ., & Bitiş, L. (2022). In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L. International Journal of Agriculture Environment and Food Sciences, 6(3), 389-395. https://doi.org/10.31015/jaefs.2022.3.7