Stellaria media Ekstraktlarının Antioksidan Aktivitesinin Belirlenmesi ve Fenolik Bileşenlerinin Karakterizasyonu

Year 2018, Volume: 8 Issue: 2, 165 - 173, 31.12.2018

Akgül Rakhimzhanova Özge Kılınçarslan Ramazan Mammadov

Abstract

Caryophyllales ailesine üye olan Stellaria media L. türünün etanol ve su
ile ekstraktları hazırlanarak toplam fenolik, flavonoid ve antioksidan
aktiviteleri belirlenmiştir. β-Karoten/ Linoleik Asit
Yöntemine göre (%59.09±1.66) ve CUPRAC
yöntemine (IC₅₀:1.19±0.02) göre en yüksek toplam antioksidan kapasite; ve en
yüksek DPPH serbest radikal giderim aktivitesi (IC₅₀:
49.72 ±0.57 mg/ml)  etanol
ekstraktlarında gözlemlenmiştir. En
yüksek toplam fenolik (21.43±0.12 mgGAE/g) ve flavonoid (22.300±1.45 mgQE/g)
madde miktarları etanol ekstraktlarında tespit edilmiştir. Ayrıca HPLC metodu
ile etanol ekstraktlarının fenolik bileşenleri tespit edilmiştir. Fenolik bileşen karakterizasyon sonuçlarına göre en yüksek
miktarda tespit edilen fenolik bileşenlerin; epikateşin (1210.99 μg/g) ve p-kumarik asit (506.99 μg/g) olduğu
ortaya çıkarılmıştır.

Keywords

Stellaria media, antioksidan, fenolik, flavonoid, HP LC

References

• 1. Ahmad S S & Husain S Z (2008). Ethno medicinal survey of plants from salt range (Kallar Kahar) of Pakistan. Pak J Bot. 40: 1005-1011
• 2. Amin I & Hong T (2002). Antioxidant activity of selected commercial seaweeds. Malays. J. Nutr. 8(2): 167-177
• 3. Apak R, Guclu K, Ozyurek M, Karademir S E & Ercag E (2006). The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition 57(5/6): 292-304
• 4. Arnous A, Makris D P & Kefalas P (2001). Effect of principal polyphenoliccomponents in relation to antioxidant characteristics of aged redwines. J. Agric. Food. Chem. 49: 5736-5742
• 5. Arvouet-Grand A, Vennat B, Pourrat A & Legret P (1994). Standardization of propolis extract and identification of principal constituents. Journal de Pharmacie de Belgique 49(6): 462-468
• 6. Baytop T (1984). Türkiye’de Bitkiler ile Tedavi (Treatment with Plants in Turkey). Istanbul Universitesi Eczacılık Fakültesi Yayınları: 40, Istanbul, TR
• 7. Bukola O & Bernard A. (2011). Phytochemistry and in vitro anti-oxidant activities of Stellaria media, Cajanus cajan and Tetracera potatoria methanolic extracts. Journal of Medicinal Plants Research 5(30): 6622-6627
• 8. Caponio F, Alloggio V & Gomes T (1999). Phenolic compounds of virgin olive oil: influence of pastepreperation techniques. Food Chemistry 64: 203-209
• 9. Cui H, Kong Y & Zhang H (2012). Oxidative stress, mitochondrial dysfunction, and aging. Journal of signal transduction 2012
• 10. Fitzpatrick F K (1954). Plant substances achieve against Mycobacterium tuberculoses, Antibiotics and Chemotherapy 4
• 11. Güner A, Aslan S, Ekim T, Vural M & Babaç M T (2012). Plants list of Turkey. Researches of Flora (in Turkish), Istanbul
• 12. Hodisan V & Sancraian A (1989). Triterpenoid saponins from Stellaria media (L.) Cyr. Farmacia 37: 105-109
• 13. Kitanov G (1992). Phenolic acids and flavonoids from Stellaria media (L.) Vill. (Caryophyllaceae). Pharmazie 47:470-471
• 14. Koubajer B H, Snoussi H, Essaidi I, Chaabouni M M, Thonart P & Bouzouita N (2014). Betalain and phenolic compositions, antioxidant activity of Tunisian red beet (Beta vulgaris L. conditiva) roots and stems extracts. International journal of food properties 17(9),1934-1945
• 15. Lu F & Foo L Y (1995). Toxicological aspects of food antioxidants. Food antioxidants. New York: Marcel Dekker
• 16. Mahdavi M, Assadi M, Fallahian F & Nejadsattari T (2012). The systematic significance of seed Micro-Morphology in Stellaria L.(Caryophyllaceae) and its closest relatives in Iran. Iranian journal of Botany 18(2): 302-310
• 17. Malik A H, Khuroo A A, Dar G H & Khan Z S (2011). Ethnomedicinal uses of some plants in the Kashmir Himalaya. Ind J Tradit Know. 10: 362- 366
• 18. Mammadov R, Ili P, Ertem Vaizoğulları H & Afacan Makascı A (2011). Antioxidant activity and total phenolic content of Gagea fibrosa and Romulea ramiflora. Iran. J. Chem. Chem. Eng. 30(3), 57-62
• 19. Mates J M, Perez-Gomez C & Nunez de Castro I (1999). Antioxidant enzymes and human diseases. Clin. Biochem. 32: 595–603
• 20. Miller N J & Rice-Evans C A (1997). The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidants to the activity of orange and apple fruit juices and black currant drink. Food. Chem. 60: 331-337
• 21. Rani N, Vasudeva N & Sharma S K (2012). Quality assessment and anti-obesity activity of Stellaria media (Linn.) Vill. BMC complementary and alternative medicine, 12(1), 145
• 22. Rice-Evans C A, Miller N J, Bolwell P G, Bramley P M & Pridham J B (1995). The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free. Radical. Res. 23: 375-383
• 23. Rogowska M, Lenart M, Srečec S, Ziaja M, Parzonko A & Bazylko A (2017). Chemical composition, antioxidative and enzyme inhibition activities of chickweed herb (Stelaria media L., Vill.) ethanolic and aqueous extracts. Industrial Crops and Products, 97: 448-454
• 24. Salam J S, Joylani S D, Rebika N D & Priyadarshini S (2011). Secondary Metabolites, Antioxidant Status and Nutritive Composition of Two Non-Conventional Leafy Vegetables-Stellaria media L. and Chenopodium album L. Indian Journal of Agricultural Biochemistry 24(2):136-140
• 25. Sarmah P, Sarma, A, Kashyap D, Mahanta M & Medhi P (2014). Nutraceutical properties of Stellaria media (L.) Vill. and Persicaria chinensis (L.) H. Gross under Brahmaputra valley agro-climatic condition. Annals of Plant Sciences 3(08): 779-782
• 26. Scully V (1960). A Treasury of American Indian Herbs. Bonanza Books: 210-213, New York
• 27. Sharma A & Arora D (2012). Phytochemical and pharmacological potential of genus Stellaria: a review. J. Pharma. Res. 5(7): 3591-3596
• 28. Shi J, Nawaz H, Pohorly J, Mittal G, Kakuda Y & Jiang Y (2005). Extraction of polyphenolics from plant material for functional foods-engineering and technology. Food Rev. Int. 21:139–166
• 29. Shinwari M I & Khan M A (2000). Folk use of medicinal herbs of Margalla Hills National Park, Islamabad. J Ethnopharmacol 69: 45- 56
• 30. Singh B & Yadav S K (2010). In Vitro studies on antibacterial activity and phytochemical analysis of whole plant extracts of Stellaria media. International Journal of Phytomedicine 2: 260–266
• 31. Slinkard K & Singleton V L (1977). Total phenol analysis: automation and comparison with manual methods. Am. J. Enol. Vitic. 28:49–55
• 32. Wu C, Chen F, Wang X, Kim H J, He G Q, Haley-Zitlin V & Huang G (2006). Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chemistry 96(2): 220-227
• 33. Yong-Mei H, Wen-Cai Y, Qian L, Hai-Yan T, Hao W & Hong-Yu D (2006). C-glycosylflavones from Stellaria media, Zhongguo Tianran Yaowu 4: 420-424
• 34. Zengin G, Mahomoodally M F, Aktümsek A, Ceylan R, Uysal S, Mocan A, Yılmaz M A, Picot-Allain C M N, Ciric A, Glaoclija J & Sokovic M (2018). Functional constituents of six edible Silene species: A focus on their phytochemical profiles and bioactive properties. Food Bioscience 23: 75-82