Stellaria media Ekstraktlarının Antioksidan Aktivitesinin Belirlenmesi ve Fenolik Bileşenlerinin Karakterizasyonu

Year 2018, Volume: 8 Issue: 2, 165 - 173, 31.12.2018

Akgül Rakhimzhanova Özge Kılınçarslan , Ramazan Mammadov

Abstract

Caryophyllales ailesine üye olan Stellaria media L. türünün etanol ve su
ile ekstraktları hazırlanarak toplam fenolik, flavonoid ve antioksidan
aktiviteleri belirlenmiştir. β-Karoten/ Linoleik Asit
Yöntemine göre (%59.09±1.66) ve CUPRAC
yöntemine (IC₅₀:1.19±0.02) göre en yüksek toplam antioksidan kapasite; ve en
yüksek DPPH serbest radikal giderim aktivitesi (IC₅₀:
49.72 ±0.57 mg/ml)  etanol
ekstraktlarında gözlemlenmiştir. En
yüksek toplam fenolik (21.43±0.12 mgGAE/g) ve flavonoid (22.300±1.45 mgQE/g)
madde miktarları etanol ekstraktlarında tespit edilmiştir. Ayrıca HPLC metodu
ile etanol ekstraktlarının fenolik bileşenleri tespit edilmiştir. Fenolik bileşen karakterizasyon sonuçlarına göre en yüksek
miktarda tespit edilen fenolik bileşenlerin; epikateşin (1210.99 μg/g) ve p-kumarik asit (506.99 μg/g) olduğu
ortaya çıkarılmıştır.

Keywords

Stellaria media, antioksidan, fenolik, flavonoid, HP LC

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