Evaluation of thiol-disulphide homeostasis in patients with chronic hepatitis B

Year 2019, , 396 - 403, 01.12.2019

Fikriye Milletli-sezgin , Rukiye Nar , Lokman Hızmalı

https://doi.org/10.21601/ortadogutipdergisi.505078

Abstract

Aim: To evaluate the relationship between dynamic thiol-disulfide homeostasis which is a novel oxidative stress marker and levels of HBV DNA, and the oxidant-antioxidant balance.
Material and Method: In the controlled study which included chronic hepatitis B (CHB) patients and healthy volunteers, dynamic Thiol-disulphide homeostasis (TDH) was measured using a novel automated method developed by Erel. Disulfide / total thiol (%), disulfide / native thiol (%), and native thiol / total thiol (%) rates were calculated using the previously determined concentrations of disulfides, native thiols, and total thiols.
Results: Of thiol / disulfide homeostasis parameters, native thiol, total thiol, and disulfide levels were statistically lower in the CHB patient group (p <0.05). As a result of the correlation analyses, a significant negative correlation was determined between HBV DNA levels and disulfide / native thiol, disulfide / total thiol, and native thiol / total thiol parameters (p <0.05).
Conclusions: Our results suggest that oxidative stress increases with the rise in HBV-DNA levels and that the antioxidant defense may have weakened.

Keywords

chronic hepatitis B, thiol-disulfide homeostasis, HBV-DNA

Kronik hepatit B hastalarında tiyol-disülfid hemostazının değerlendirilmesi

Abstract

Amaç: HBV DNA seviyeleri ile yeni bir oksidatif stres belirteci olan dinamik tiyol-disülfit homeostazı ve oksidan-antioksidan dengesi arasındaki ilişkiyi değerlendirmektir.
Gereç ve Yöntem: Kronik hepatit B (KHB) hastalarını ve sağlıklı gönüllüleri içeren kontrollü çalışmada, dinamik Tiyol-disülfid hemostazı (TDH), Erel tarafından geliştirilen yeni bir otomatik yöntem kullanılarak ölçüldü. Disülfid / total tiyol (%), disülfid / nativ tiyol (%) ve nativ tiyol / total tiyol (%) oranları, önceden belirlenmiş disülfid konsantrasyonları, nativ tiyoller ve total tiyoller kullanılarak hesaplandı.
Bulgular: Tiyol / disülfit homeostazı parametrelerinde, nativ tiyol, total tiyol ve disülfit seviyeleri KHB hasta grubunda istatistiksel olarak düşüktü (p <0.05). Korelasyon analizleri sonucunda HBV DNA düzeyleri ve disülfit / nativ tiyol, disülfit / total tiyol ve nativ tiyol / total tiyol parametreleri arasında anlamlı bir negatif korelasyon tespit edildi (p <0.05).
Sonuç: Sonuçlarımız oksidatif stresin HBV-DNA seviyelerinin yükselmesiyle arttığını ve antioksidan savunmanın zayıflamış olabileceğini göstermektedir.

Keywords

kronik hepatit B, tiyol-disülfid hemostazı, HBV-DNA

References

• Thomas D, Zoulim F. New challenges in viral hepatitis. Gut 2012;61:1-5.
• World Health Organization. Prevention and Control of Viral Hepatitis Infection: Framework for Global Action. Geneva: WHO, 2012. http://apps.who.int/iris/bitstream/handle/10665/130012/WHO_HSE_PED_HIP_GHP_2012.1_eng.pdf;jsessionid=702912B1D626C665E81559E74B8CAE49?sequence=1
• Yapali S, Talaat N, Lok AS. Management of hepatitis B: our practice and how it relates to the guidelines. Clin Gastroenterol Hepatol 2014;12:16-26.
• Lok AS. Serologic diagnosis of hepatitis B virus infection. Waltham, MA: UpToDate, Inc. http://www.uptodate.com/contents/serologic-diagnosis-of-hepatitis-bvirus-infection
• Janssen-Heininger YM, Mossman BT, Heintz NH, et al. Redox-based regulation of signal transduction: principles, pitfalls, and promises. Free Radic Biol Med 2008;45:1–17.
• Thanan R, Oikawa S, Hiraku Y, et al. Oxidative Stress and Its Significant Roles in Neurodegenerative Diseases and Cancer. Int J Molecul Sci 2014;16:193–217.
• Cai Z, Yan LJ. Protein Oxidative Modifications: Beneficial Roles in Disease and Health. J Biochem Pharmacol Re. 2013;1:15–26.
• Esen R, Aslan M, Kucukoglu ME, et al. Serum paraoxonase activity, total thiols levels, and oxidative status in patients with acute brucellosis. Wien Klin Wochenschr 2015;127:427–33.
• Dundaroz R, Erenberk U, Turel O, Demir AD, Ozkaya E, Erel O. Oxidative and antioxidative status of children with acute bronchiolitis. J Pediatr (Rio J) 2013;89:407–11.
• Karsen H, Sunnetcioglu M, Ceylan RM, et al. Evaluation of oxidative status in patients with Fasciola hepatica infection. Afr Health Sci 2011;11:S14–8.
• Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 2000;72:653–69.
• Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013;288:26489–96.
• Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med 2009;47:1329–38.
• Biswas S, Chida AS, Rahman I. Redox modificationsof protein-thiols: emerging roles in cell signaling. Biochem Pharmacol 2006;71:551-64.
• Altıparmak IH, Erkuş ME, Sezen H, et al. The relation of serum thiol levels and thiol/disulphide homeostasis with the severity of coronary artery disease. Kardiol Pol 2016;74:1346-53.
• Ateş I, Kaplan M, Yüksel M, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016;51:47-51.
• Himmelfarb, J., McMonagle, E. and McMenamin, E. Plasma Protein Thiol Oxidation and Carbonyl Formation in Chronic Renal Failure. Kidney International 2000;58:2571-2578.
• Kaplan M, Ates I, Yuksel M, et al. Thiol/disulphide homeostasis in celiac disease. World J Gastrointest Pharmacol Ther 2017;8:120-26.
• Hanikoglu F, Hanikoglu A, Kucuksayan E, et al. Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer. Free Radic Res 2016 Nov;50:79-84.
• Gümüşyayla S, Vural G, Bektaş H, Deniz O, Neşelioğlu S, Erel O. A novel oxidative stress marker in patients with Alzheimer’s disease: dynamic thiol-disulphide homeostasis. Acta Neuropsychiatr 2016;28:315-20.
• Erel O, Neşelioğlu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32.
• Alavian SM, Showraki A. Hepatitis B and its relationship with oxidative stress. Hepatitis monthly 2016;16:e37973.
• Sen V, Uluca U, Ece A, et al. Serum prolidase activity and oxidant-antioxidant status in children with chronic hepatitis B virus infection. Ital J Pediatr 2014;40:95.
• Mahdy KA, Abd-El-Shaheed A, Khadr ME, El-Shamy KA. Antioxidant status and lipid peroxidation activity in evaluating hepatocellular damage in children. East Mediterr Health J 2009;15:842–52.
• Duygu F, Karsen H, Aksoy N, Taskin A. Relationship of oxidative stress in hepatitis B infection activity with HBV DNA and fibrosis. Ann Lab Med 2012;32:113–18.
• Severi T, Ying C, Vermeesch JR, et al. Hepatitis B virus replication causes oxidative stress in HepAD38 liver cells. Mol Cell Biochem 2006;290:79–85.
• Bhargava A, Khan S, Panwar H, et al. Occult hepatitis B virus infection with low viremiainduces DNA damage, apoptosis and oxidative stress in peripheral blood lymphocytes. Virus Res 2010;153:143–50.
• Wang K, Han L, Lu Q,Wang B, Li X,Wang H. Nitric oxide and nitric oxide synthase in patients with chronic hepatitis B. Zhonghua shi yan he lin chuang bing du xue za zhi= Zhonghua shiyan he linchuang bingduxue zazhi. Chinese J Experiment Clin Virol 2005;19:142–45.
• Tsai SM, Lin SK, Lee KT, et al. Evaluation of redox statuses in patients with hepatitis B virus-associated hepatocellular carcinoma. Ann Clin Biochem 2009;46:394–400.
• Tasdelen Fisgin N, Aydin BK, Sarikaya H, et al. Oxidative stress and antioxidant defense in patients with chronic hepatitis B. Clin Lab 2012;58:273–80.
• Guler SA, Tolun FI, Ucmak H, Haskaya G, Yavuz M, Yavuz M. Relationship between antioxidant capacity and oxidative stress in patients with chronic hepatitis B. Advance Laboratory Med Int 2014;4:17–25.
• Dalle-Donne I, Giustarini D, Colombo R, Rossi R, Milzani A. Protein carbonylation in human diseases. Trends Mol Med 2003;9:169–76.
• Namiduru ES, Namiduru M, Tarakcioglu M, Tanriverdi M. Levels of malondialdehyde, myeloperoxidase and nitrotyrosine in patients with chronic viral hepatitis B and C. Adv Clin Exp Med 2012;21:47–53.
• Meng LZ, Gao AW, Bian JF, Han LJ, Chen H. Study on Correlation between Oxidation-antioxidation and Chronic Hepatitis Viral B. Occupation Health, 2010;23.
• Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med 2013;65:244–53.
• Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Rep. 2016 Sep;21:197-203.