The strong relationship between disease severity and thiol-disulphide homeostasis in patients with restless legs syndrome

Year 2018, Volume: 10 Issue: 4, 436 - 443, 01.12.2018

Salim Neşelioğlu , Yasemin Eren Ebru Bilge Dirik Emine Feyza Yurt Serpil Erdogan , Orhan Deniz Özcan Erel

https://doi.org/10.21601/ortadogutipdergisi.475395

Abstract

Objective: This study was conducted to investigate thiol-disulphide homeostasis, a novel oxidative stress marker in primary restless legs syndrome (RLS) patients.

Material and Method: Thirty-eight patients and 43 healthy subjects were included in the study. Serum native thiol, total thiol, disulphide and disulphide / native thiol percent ratio levels were measured by using a novel automated method. Diagnosis of RLS was based on the criteria proposed by the International Restless Legs Syndrome Study Group (IRLSSG), and RLS severity was assessed using the IRLSSG Severity Scale.

Results: Serum native thiol and total thiol levels were significantly lower (p< 0.001; p= 0.007, respectively) while disulphide and disulphide/native thiol percent ratio levels significantly higher (p= 0.017; p< 0.001, respectively), in patients with RLS compared with control group. Significant correlations were found between the IRLSSG Severity Scale and thiol-disulphide homeostasis parameters (r= -0.56, p< 0.001 for native thiol; r= -0.51, p< 0.001 for total thiol; r= 0.37, p= 0.020 for disulphide and r= 0.60, p< 0.001 for disulphide/native thiol percent ratio).

Conclusions: Decreased native thiol, increased disulphide levels and increased disulphide/native thiol percent ratio show that thiol-disulphide balance shifted to the oxidation side. Strong correlations between the IRLSSG Severity Scale and thiol-disulphide homeostasis parameters indicate that homeostasis may have a role in the pathogenesis of the Primary RLS.

Keywords

Disulphide, oxidative stress, restless leg syndrome, thiol, thiol-disulphide hemeostasis

Huzursuz bacak sendromlu hastalarda hastalık şiddeti ve tiyol-disülfid homeostazı arasındaki güçlü ilişki

Abstract

Amaç:
Bu çalışma, primer huzursuz bacak sendromu (RLS) hastalarında yeni bir
oksidatif stres belirteci olan tiyol-disülfid homeostazisini araştırmak
amacıyla yapıldı.

Gereç
ve Yöntem: Otuz sekiz hasta ve 43 sağlıklı birey çalışmaya
alındı. Serum nativ tiyol, total tiyol, disülfit ve disülfid / nativ tiyol
oranları yeni bir otomatik yöntem kullanılarak ölçülmüştür. RLS tanısı
Uluslararası Huzursuz Bacak Sendromu Çalışma Grubu (IRLSSG) tarafından önerilen
kriterlere gore konuldu ve RLS şiddeti IRLSSG Şiddet Ölçeği kullanılarak
değerlendirildi.

Bulgular:
Serum nativ tiyol ve total tiyol düzeyleri anlamlı derecede düşük (sırasıyla
p<0.001; p=0.007), disülfid ve disülfid / nativ tiyol oranı anlamlı olarak
daha yüksekti (sırasıyla p=0.017; p<0.001). RLS kontrol grubu ile
karşılaştırıldı. IRLSSG Önem Ölçeği ve tiyol-disülfid homeostasisi
parametreleri arasında anlamlı korelasyonlar bulundu (r=-0.56, nativ tiyol için
p<0.001; total tiyol için r=-0.51, p<0.001; disülfid için r=0.37, p=0.020);
r=0.60, disülfid / nativ tiyol yüzdesi oranı için p<0.001).

Sonuçlar:
Azalmış nativ tiyol, artan disülfid seviyeleri ve artmış disülfid / nativ tiyol
oranı, tiyol-disülfür dengesinin oksidasyon tarafına kaydığını göstermektedir.
IRLSSG Önem Ölçeği ve tiyol-disülfid homeostasisi parametreleri arasındaki
güçlü korelasyonlar, homeostazın primer RLS'nin patogenezinde rol
oynayabileceğini göstermektedir.

Keywords

Disulphide, oxidative stress, restless legs syndrome, thiol, thiol-disulphide homeostasis

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