Büllöz pemfigoidli hastalarda serum ve lezyonel miRNA-1291 ekspresyonlarının karşılaştırılması

Year 2022, , 836 - 844, 01.10.2022

Şule Subaşı Gökşin , Buket Er Urgancı , Işıl Göğem İmren , İbrahim Açıkbaş

https://doi.org/10.31362/patd.1174388

Abstract

Amaç: Büllöz pemfigoid (BP), yaşla birlikte artan otoimmün bir hastalıktır. miRNA-1291, birçok immün aracılı kutanöz hastalıkta önemli rol oynayan bir mikroRNA'dır. Serum ve lezyonlu derideki ekspresyon seviyelerini ve ana BP biyobelirteçleri (anti-BP180 ve anti-BP230) ve CCL17 ile korelasyonunu karşılaştırarak miRNA-1291'in BP'deki rolünü araştırmayı amaçladık.
Gereç ve yöntem: BP'li 23 hasta ve 23 sağlıklı kontrol hastası çalışmaya dahil edildi. Serum ve biyopsi örneklerinden RNA izolasyonu için miRNeasy Mini Kitleri kullanıldı. cDNA'nın dönüştürülmesi için Qiagen-miRCURY LNA RT Kiti kullanıldı. miRNA-1291 ekspresyonu, orijinal primer ve SYBR Green ana karışımı ile Real-Time PCR ile gerçekleştirildi. Serum CCL17, anti-BP180 ve anti-BP230 seviyeleri ELISA kullanılarak ölçüldü. İstatistiksel değerlendirme için SPSS 25.0 (IBM SPSS Statistics 25 yazılımı) kullanıldı.
Bulgular: Serum ve biyopsi örneklerinde miRNA-1291 ekspresyonu, kontrol grubuna göre sırasıyla 7.02 ve 4.77 kat anlamlı şekilde up-regüleydi (p=0,001, p=0.007). BP ve CCL17 hastalarında anti-BP-180, anti-BP-230 serum düzeyleri kontrol grubuna göre anlamlı derecede yüksekti (p=0,0049, p=0,0071, p=0,001). BP hastalarında anti-BP180, anti-BP230, CCL17 seviyeleri, serum miRNA-1291 ekspresyonu ile pozitif korelasyon gösterdi. Ek olarak, serum miRNA-1291 ekspresyonu, lezyonel miRNA-1291 ekspresyonu ile iyi korele idi.
Sonuç: Serum ve lezyonlu dokuda lezyonlu olmayan dokuya göre miRNA-1291 düzeylerinin artması, miRNA-1291'in BP'nin patogenezinde rol oynayabileceğini düşündürmektedir. miRNA-1291 diğer BP biyobelirteçleri ile korelasyon gösterdiğinden, BP için önemli bir biyobelirteç olarak hizmet edebilir.

Keywords

Büllöz pemfigoid, , biyobelirteç, miRNA-1291, BP180, CCL17

Supporting Institution

Pamukkale Üniversitesi

Project Number

2020BSP20

Comparison of serum and lesional miRNA-1291 expressions in patients with bullous pemphigoid

Abstract

Purpose: Bullous pemphigoid (BP) is an autoimmune disease increasing by age. miRNA-1291 is a microRNA that plays major role in many immune-mediated cutaneous diseases. We aimed to investigate the role of miRNA-1291 in BP by comparing its expression levels in serum and lesional skin and its correlation with main BP biomarkers (anti-BP180, and anti-BP230) and CCL17
Materials and methods: Twenty three patients with BP and 23 healthy control patients were included in the study. The miRNeasy Mini Kits was used for RNA isolation from serum and biopsy samples. Qiagen-miRCURY LNA RT Kit was used for conversion of cDNA. miRNA-1291 expression was performed by Real-Time PCR with the original primer and SYBR Green master mix. Serum CCL17, anti-BP180, and anti-BP230 levels were measured with use of ELISA. SPSS 25.0 (IBM SPSS Statistics 25 software) was used for statistical evaluation.
Results: The expression of miRNA-1291 in serum and biopsy samples were 7.02 and 4.77-fold significantly upregulated than that of control group respectively (p=0.001, p=0.007). The serum levels of anti-BP-180, anti-BP-230 and CCL17 in BP patients were significantly higher compared to control group (p=0.0049, p=0.0071, p=0.001). Anti-BP180, anti-BP230, CCL17 levels and serum miRNA-1291 expression were correlated positively in BP patients. Additionally, serum miRNA-1291 expression were correlated well with lesional miRNA-1291 expression.
Conclusion: The increase in miRNA-1291 levels in serum and lesional tissue compared to non-lesional tissue suggests that miRNA-1291 may play a role in the pathogenesis of BP. As miRNA-1291 correlates with other BP biomarkers, it may serve as an important biomarker for BP.

Keywords

Bullous pemphigoid, biomarker, miRNA-1291, BP180, CCL17

Project Number

2020BSP20

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