Parkinson tanısı almış hastalarda snoRNA’ların deregülasyonu

Year 2023, , 111 - 119, 31.01.2023

Pervin Elvan Tokgün , Ayşe Gaye Tomatır , Fatma Gizem Sarıekiz , Sinan Bir

https://doi.org/10.31362/patd.1218005

Abstract

modifikasyonlarına aracılık ederler. 5'-uçları ve poli-A kuyrukları yoktur. C/D box snoRNA'lar, H/ACA box
snoRNA'lar ve küçük kajal gövdesine özgü RNA'lar olarak sınıflandırılırlar. snoRNA'lar, transkripsiyon, RNA
eklenmesi, hücre döngüsü vb. gibi önemli biyolojik süreçlerde önemli rollere sahiptir. Bu çalışmada, Parkinson
tanısı alan hastaların PBMC'lerinde snoRNA'ların ifade değişimlerini mikroarray analizi ile ortaya koymaya
çalıştık.
Gereç ve yöntem: Unilateral başlangıç öyküsü olan ve ilk yıllarda dopaminerjik tedaviye iyi yanıt verdiği
düşünülen hastalar (n=3) çalışmaya alındı. Periferik kan mononükleer hücre izolasyonu için 10 ml periferik kan
örneği alındı. Total RNA, GeneAll® Hybrid-R™ kiti kullanılarak izole edildi ve Affymetrix GeneChip Human ST
2.0 platformu kullanılarak mikrodizin analizi yapıldı. Ham datalar, Affymetrix Command Console Software 1.1
kullanılarak çıkarıldı. KEGG yolak ve Gen ontoloji analizleri yapıldı ve lokus genlerin protein-protein değişimi
STRING veri tabanı kullanılarak gerçekleştirildi.
Bulgular: Elde edilen veriler, 28 snoRNA'nın upregüle olduğunu ve 3 snoRNA'nın downregüle olduğunu ortaya
çıkardı
Sonuç: Bu çalışmada, mikrodizin analizi ile kesin Parkinson tanısı alan hastalarda snoRNA'ların ifade
değişimlerini değerlendirdik ve bazı snoRNA'ların deregüle ekspresyonlarını gözlemledik. snoRNA'lardaki ifade
değişimi, lokus genlerin transkripsiyonel aktivitesinde değişikliklere neden olabilir ve bu nedenle hastalıklar için
biyobelirteçler olarak değerlendirilebilirler.

Keywords

parkinson, snoRNA, mikrodizin

Supporting Institution

Pamukkale üniversitesi Bilimsel Araştırma Projeleri koordinatörlüğü

Project Number

2020SABE024

snoRNAs are deregulated in patients with Parkinson’s Disease

Abstract

Purpose: Small nucleolar RNAs are ranging from 65 to 300 nucleotides in length that mediate post-transcriptional
RNA modifications. They don’t have a 5′-Cap and a poly-A tail and are categorized as C/D box snoRNAs, H/
ACA box snoRNAs, and small Cajal body-specific RNAs. snoRNAs have essential roles in important biological
processes such as transcription, RNA splicing, cell cycle, and etc. In this study, we tried to reveal differential
expressions of snoRNAs in PBMCs of patients with Parkinson’s Disease by microarray analysis.
Materials and methods: Patients (n=3) who are considered to have a unilateral onset history and a good
response to dopaminergic treatment in the first years were included in the study. 10 ml peripheral blood sample
was taken for peripheral blood mononuclear cell isolation. Total RNA was extracted using GeneAll® Hybrid-R™
kit and microarray analysis was performed by using Affymetrix GeneChip Human ST 2.0 platform. Raw data
were extracted using Affymetrix Command Console Software 1.1. KEGG pathway and GO terms analyses were
performed and protein-protein interaction of host genes were determined by using STRING database.
Results: Data from patients revealed that there were 28 snoRNAs were downregulated and 3 snoRNAs were
upregulated.
Conclusion: Here in this study, we evaluated the differential expressions of snoRNAs in patients with a definitive
diagnosis of PD by microarray analysis and observed deregulated expressions of some snoRNAs. Differential
expression of snoRNA may cause changes in the transcriptional activity of host genes and thus can serve as
biomarkers for diseases.

Keywords

parkinson, snoRNA, microarray

Project Number

2020SABE024

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