Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin

Hülya Çetin; Damla Gündüz; Hülya Aybek

doi:10.31362/patd.1268311

EN TR

Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin

Abstract

Purpose: Gentamicin is an antibiotic, used to treat infections caused by microorganisms. Endogenous antioxidants are strengthened by using antioxidant protectors against the toxic effects caused by drugs. Rosmarinic acid has antioxidative, anti-inflammatory, antiapoptotic, and antitumoral effects. In our study, we aimed to investigate the protective effect, immunohistochemical changes, oxidative markers, histopathological changes and inflammation related Ifi44 expression of rosmarinic acid against gentamicin induced nephrotoxicity in rats. Materials and methods: In our study, 32 rats randomly divided into four groups. These groups are consisted of control group, gentamicin group received gentamicin 100 mg/kg/day, gentamicin + rosmarinic group acid received gentamicin 100 mg/kg/day and rosmarinic acid 50 mg/kg/day, and rosmarinic acid group received rosmarinic acid 50 mg/kg/day. At the end of the study, histopathological, immunohistochemical, and biochemical changes in kidney tissues evaluated. Results: Rosmarinic acid reduced creatine, urea, blood urea nitrogen, and total oxidative stress in blood serum. The toxic effect of gentamicin caused severe histopathological changes in the kidneys. A slight decrease in histopathological changes observed in the gentamicin + rosmarinic acid group. Antiproliferative Ifi44 expression was higher in the gentamicin group and gentamicin + rosmarinic acid group. Conclusion: As a result of the application of rosmarinic acid, a decrease in oxygen radicals and an increase in antioxidant levels observed. When used in combination with gentamicin and rosmarinic acid, the protective effect of rosmarinic acid was partially observed, but it could not provide full protection.

Keywords

Gentamicin, rosmarinic acid, Ifi44, rat

Project Number

2019SABE014

Yüksek doz gentamisin verilen sıçanlarda rosmarinik asidin koruyucu etkisinin araştırılması

Abstract

Amaç: Gentamisin, mikroorganizmaların neden olduğu enfeksiyonları tedavi etmek için kullanılan bir antibiyotiktir. İlaçların neden olduğu toksik etkilere karşı antioksidan koruyucular kullanılarak endojen antioksidanlar güçlendirilir. Rosmarinik asit antioksidan, antienflamatuar, antiapoptotik ve antitümör etkilere sahiptir. Çalışmamızda rosmarinik asidin sıçanlarda gentamisin ile indüklenen nefrotoksisiteye karşı koruyucu etkisini, immünohistokimyasal değişiklikleri, oksidatif belirteçleri, histopatolojik değişiklikleri ve enflamasyona bağlı Ifi44 ekspresyonunu araştırmayı amaçladık. Gereç ve yöntem: Çalışmamızda 32 rat rastgele 4 gruba ayrıldı. Bu gruplar kontrol grubu, gentamisin grubu 100 mg/kg/gün gentamisin verildi, gentamisin + rosmarinik asit grubu gentamisin 100 mg/kg/gün ve rosmarinik asit 50 mg/kg/gün verildi, rosmarinik asit grubu rosmarinik asit 50 mg/kg/gün verildi. Çalışma sonunda böbrek dokularındaki histopatolojik, immünohistokimyasal ve biyokimyasal değişiklikler değerlendirildi. Bulgular: Rosmarinik asit, kan serumunda kreatin, üre, kan üre nitrojeni ve toplam oksidatif stresi azaltmıştır. Gentamisinin toksik etkisi böbreklerde ciddi histopatolojik değişikliklere neden olmuştur. Gentamisin + rosmarinik asit grubunda histopatolojik değişikliklerde hafif bir azalma gözlendi. Antiproliferatif Ifi44 ekspresyonu gentamisin grubunda ve gentamisin + rosmarinik asit grubunda daha yüksekti. Sonuç: Rosmarinik asit uygulaması sonucunda oksijen radikallerinde azalma ve antioksidan düzeylerinde artış gözlendi. Gentamisin ve rosmarinik asit birlikte kullanıldığında rosmarinik asidin koruyucu etkisi kısmen gözlenmiştir ancak tam koruma sağlayamamıştır.

Keywords

Gentamisin, rosmarinik asit, Ifi44, sıçan

Supporting Institution

Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi desteği ile gerçekleştirilmiştir

Project Number

2019SABE014

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Details

Primary Language

English

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Hülya Çetin ^*
0000-0001-8731-0631
Türkiye

Damla Gündüz
0000-0003-4838-6574
Türkiye

Hülya Aybek
0000-0002-0635-4251
Türkiye

Early Pub Date

June 14, 2023

Publication Date

July 1, 2023

Submission Date

March 20, 2023

Acceptance Date

June 12, 2023

Published in Issue

Year 2023 Volume: 16 Number: 3

DOI

https://doi.org/10.31362/patd.1268311

IZ

https://izlik.org/JA53EW28FM

APA

Çetin, H., Gündüz, D., & Aybek, H. (2023). Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin. Pamukkale Medical Journal, 16(3), 486-497. https://doi.org/10.31362/patd.1268311

AMA

1.Çetin H, Gündüz D, Aybek H. Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin. Pam Med J. 2023;16(3):486-497. doi:10.31362/patd.1268311

Chicago

Çetin, Hülya, Damla Gündüz, and Hülya Aybek. 2023. “Investigation of the Protective Effect of Rosmarinic Acid in Rats Given High Dose Gentamicin”. Pamukkale Medical Journal 16 (3): 486-97. https://doi.org/10.31362/patd.1268311.

EndNote

Çetin H, Gündüz D, Aybek H (July 1, 2023) Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin. Pamukkale Medical Journal 16 3 486–497.

IEEE

[1]H. Çetin, D. Gündüz, and H. Aybek, “Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin”, Pam Med J, vol. 16, no. 3, pp. 486–497, July 2023, doi: 10.31362/patd.1268311.

ISNAD

Çetin, Hülya - Gündüz, Damla - Aybek, Hülya. “Investigation of the Protective Effect of Rosmarinic Acid in Rats Given High Dose Gentamicin”. Pamukkale Medical Journal 16/3 (July 1, 2023): 486-497. https://doi.org/10.31362/patd.1268311.

JAMA

1.Çetin H, Gündüz D, Aybek H. Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin. Pam Med J. 2023;16:486–497.

MLA

Çetin, Hülya, et al. “Investigation of the Protective Effect of Rosmarinic Acid in Rats Given High Dose Gentamicin”. Pamukkale Medical Journal, vol. 16, no. 3, July 2023, pp. 486-97, doi:10.31362/patd.1268311.

Vancouver

1.Hülya Çetin, Damla Gündüz, Hülya Aybek. Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin. Pam Med J. 2023 Jul. 1;16(3):486-97. doi:10.31362/patd.1268311

Pamukkale Medical Journal is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY NC).

Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin

Abstract

Keywords

Project Number

Yüksek doz gentamisin verilen sıçanlarda rosmarinik asidin koruyucu etkisinin araştırılması

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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