Determining the levels of serum Heat Shock Protein B7 (HSPB7) and tetranectin in patients undergoing hemodialysis

Abstract

Purpose: Heart damage may develop over time in patients with chronic renal disease (CKD), who are undergoing hemodialysis (HD) treatment. Serum levels of heat shock protein B7 (HSPB7) and tetranectin proteins change following damage to the heart muscle. This study aimed to determine HSPB7 and tetranectin levels in patients with CKD undergoing HD treatment. Materials and methods: The patients aged 30-60 years old, healthy controls (n=30) and HD patients (n=30) participated in the study. Blood samples were taken from healthy subjects who applied to the hospital for check-up and from patients with kidney disease receiving HD treatment. Biochemical parameters were examined from the blood taken. HSPB7 and tetranectin levels from isolated serum samples were determined using measurement kits based on the solid phase sandwich (ELISA) principle. Results: There was no significant difference between the groups in gender, age, glucose and iron (Fe) values of the subjects (p>0.05). Lymphocyte, platelet counts, hemoglobin and albumin values were found to be lower in patient group compared to the control group (p<0.05). Urea, creatine kinase (CK) and C-reactive protein (CRP) values were found to be higher in patient group than in the control group (p<0.05). A significant increase in HSPB7 levels and a significant decrease in tetranectin levels were detected in patient group compared to control group (p<0.001). Conclusions: In this study, the changes detected in HSPB7 and tetranectin levels in with CKD undergoing HD treatment. May be early indicators of the possible development of cardiovascular diseases in patients with renal disease.

Keywords

• Hemodialysis
• chronic renal disease (CKD)
• HSPB7
• tetranectin

Hemodiyaliz tedavisi alan hastalarda serum Isı Şok Proteini B7 (HSPB7) ve tetranektin düzeylerinin belirlenmesi

Abstract

gelişebilmektedir. Isı şok proteini B7 (HSPB7) ve tetranektin proteinlerinin serum düzeyleri kalp kasında gelişen hasarı takiben değişmektedir. Bu çalışmada, HD tedavisi alan KBH hastalarında HSPB7 ve tetranektin düzeylerinin araştırılması amaçlanmıştır. Gereç ve yöntem: Çalışmaya 30-60 yaş, sağlıklı ve HD hastası denekler katılmış olup iki grup oluşturulmuştur (n=60): Kontrol grubu (n=30) ve Hasta grubu (n=30). Hastaneye check-up için başvuran sağlıklı deneklerden ve böbrek hastalığı olup HD tedavisi alan hastalardan kan örnekleri alınmıştır. Alınan kanlardan biyokimyasal parametreler incelenmiştir. İzole edilen serum örneklerinden HSPB7 ve tetranektin düzeyleri solid faz sandwich (ELİSA) prensibine dayanan ölçüm kitleri kullanılarak belirlenmiştir. Bulgular: Deneklerin cinsiyet, yaş, glukoz ve Demir (Fe) değerlerinde gruplar arasında anlamlı fark saptanmamıştır (p>0,05). Lenfosit, trombosit sayıları, hemoglobin ve albümin değerleri diyaliz hastalarında kontrol grubuna kıyasla düşük bulunmuştur (p<0,05). Diyaliz hastalarında üre, kreatin kinaz ve CRP değerleri sağlıklı kontrol grubuna göre yüksek saptanmıştır (p<0,05). Hastalarda sağlıklı deneklere kıyasla, HSPB7 düzeylerinde anlamlı artış ve tetranektin düzeylerinde ise anlamlı düşüş tespit edilmiştir (p<0,001). Sonuç: Bu çalışmada, diyaliz tedavisi alan KBH hastalarında HSPB7 ve tetranektin düzeylerinde tespit edilen değişimler, böbrek yetmezliği olan hastalarda olası gelişebilecek kardiyovasküler hastalıkların gelişiminin erken göstergesi olabilir.

Keywords

• Hemodiyaliz
• kronik böbrek hastalığı (KBH)
• HSPB7
• tetranektin

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