Yaşlı sıçanlarda borik asidin oksidan-antioksidan, proinflamatuar sitokin seviyeleri ve biyokimyasal parametreler üzerine etkisi

Year 2024, , 369 - 379, 01.04.2024

Mehmet Başeğmez , Muhammed Fatih Doğan

https://doi.org/10.31362/patd.1438593

Abstract

Amaç: Yapılan literatür çalışmaları neticesinde borik asit ’in birçok çalışmaya konu olduğu ve canlıya olan etkilerinin araştırılarak incelendiği görü lmüş tü r. Bu çalışmada yaşlı sıçanlarda farmakolojik dozlarda uygulanan oral borik asit takviyesinin fizyolojik ve biyokimyasal sistemlerdeki etkilerinin araştırılması amaçlanmıştır.
Gereç ve yöntem: Toplam 32 adet Wistar Albino erkek yaşlı sıçan rastgele ve eşit olarak aşağıdaki dört gruba ayrılmıştır: 1.; Kontrol=1ml salin, 2.; Düşük-doz borik asit (L-BA)=10 mg/kg, 3.; Orta-doz borik asit (M-BA)=20 mg/kg, 4.; Yüksek-doz borik asit (H-BA)=40 mg/kg. Yaşlı sıçanlara 28 gün boyunca borik asit oral olarak verilmiştir. Sıçanların kanı, karaciğer ve böbrek örnekleri oksidan, antioksidan, proinflamatuar sitokinler ve biyokimyasal değişiklikler açısından analiz edilmek üzere 29. günde toplanmıştır.
Bulgular: Borik asit, albümin, total protein ve kalsiyum düzeylerini kontrol grubuna kıyasla tüm borik asit gruplarında eşit oranda artırırken (p<0,05), kolesterol parametresini sadece H-BA grubunda artırmıştır (p<0,05). Borik asit fosfor düzeyini kontrol ve L-BA gruplarına kıyasla M-BA ve H-BA gruplarında artırırken (p<0,05), total bilirubin düzeyini sadece L-BA grubunda (p<0,05), kan üre nitrojen düzeyini L-BA ve M-BA gruplarında artırmıştır (p<0,05). Ayrıca alanin aminotransferaz düzeyini sadece M-BA grubunda artırırken (p<0,05), kreatin kinaz ve glukoz düzeylerini kontrol grubuna kıyasla tüm gruplarda artırdığı tespit edilmiştir (p<0,05). Ancak borik asidin globulin, kreatin, alkalin fosfataz, amilaz düzeyleri üzerinde doza bağlı bir etkisi olmamıştır (p>0,05). Borik asit karaciğer ve böbrek dokularında MDA düzeylerini önemli ölçüde azaltmış (p<0,05) ve GSH, SOD ve CAT enzim aktivitelerini artırmıştır (p<0,05). Ayrıca, borik asit plazma IL-6 ve TNF-α proinflamatuar sitokin düzeylerini azaltmıştır (p<0,05).

Keywords

Yaş, antioksidan, borik asit, oksidan, proinflamatuar sitokin

Supporting Institution

Pamukkale Üniversitesi Bilimsel Araştırma Koordinatörlüğü

Project Number

2022BSP010

Effects of boric acid on oxidant-antioxidant, proinflammatory cytokine levels, and biochemical parameters in aged rats

Abstract

Purpose: As a result of the literature studies, it was seen that boric acid was the subject of many studies, and its effects on living things were investigated and examined. This study aimed to investigate the effects of oral boric acid supplementation at pharmacologic doses on physiological and biochemical systems in aged rats.
Material and methods: A total of 32 Wistar Albino male-aged rats were randomly and equally divided into the following four groups: 1st; Control=1 ml saline; 2nd; Low-dose boric acid (L-BA)=10 mg/kg; 3rd; Medium-dose boric acid (M-BA)=20 mg/kg; 4th; High-dose boric acid (H-BA)=40 mg/kg. Boric acid was given orally to aged rats for 28 days. Blood, liver, and kidney samples of rats were collected on day 29 to be analyzed for oxidants, antioxidants, proinflammatory cytokines, and biochemical changes.
Result: Boric acid significantly increased albumin, total protein, calcium levels equally in all boric acid groups compared to the control group (p<0.05), increased cholesterol parameter only in H-BA group (p<0.05), increased phosphor level in M-BA and H-BA groups compared to control and L-BA groups (p<0.05), total bilirubin level was increased only in L-BA group (p<0.05), blood urea nitrogen level was increased in L-BA and M-BA groups (p<0.05), alanine aminotransferase level was increased only in M-BA group (p<0.05), creatine kinase and glucose levels were increased boric acid in all groups compared to control group (p<0.05). However, boric acid did not affect globulin, creatine, alkaline phosphatase, and amylase levels in a dose-dependent manner (p>0.05). Boric acid significantly decreased MDA levels (p<0.05) and increased GSH, SOD, and CAT enzyme activities (p<0.05) in liver and kidney tissues in a dose-dependent manner. In addition, boric acid decreased plasma IL-6 and TNF-α proinflammatory cytokine levels (p<0.05).
Conclusion: This study demonstrated that boric acid supplementation has ameliorative effects in a dose-dependent manner on lipid peroxidation, immunomodulation, and regulation of many blood biochemical parameters in aged rats.

Keywords

Age, antioxidant, boric acid, oxidant, proinflammatory cytokine

Supporting Institution

Pamukkale University Scientific Research Coordination Unit

Project Number

2022BSP010

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