Quercetin'in 3T3-L1 adipositlerinde lipid birikimi ve adiponektin düzeyi üzerindeki etkisinin araştırılması

Abstract

Amaç: Obezite, adipoz dokunun homeostazını bozarak adipoz hücrelerinin sayısında ve büyüklüğünde artışa neden olur. Literatürde quercetin polifenolünün çeşitli mekanizmalar aracılığıyla sağlık üzerinde pek çok olumlu etkileri bilinmektedir. Araştırmamızın amacı, quercetinin olgun ve hipertrofik adipositlerde lipit birikimi ve adiponektin (ADP) seviyeleri üzerindeki etkilerini araştırmaktır. Gereç ve yöntem: Çalışmada, 3T3-L1 preadiposit hücreler farklılaştırıldıktan sonra yüksek glikoz içeren ortamda 8 gün muamele edilerek olgun adipositler, 18 gün muamele edilerek ise hipertrofik adipositler elde edildi. Quercetin (40 ve 80 μM), olgun adipositlere 24 saat, hipertrofik adipositlere ise 48 saat boyunca uygulandı. Oil-Red-O boyama yöntemiyle lipit miktarı görüntülendi. ELISA yöntemi aracılığıyla lipit birikimi ve ADP seviyesi ölçüldü. İstatistiksel analiz için; tek yönlü varyans analizi (post hoc: Tukey testi) kullanıldı. p≤0,05 değeri istatistiksel olarak anlamlı kabul edildi. Bulgular: Olgun adipositlere her iki quercetin dozunun uygulaması lipit birikimini anlamlı olarak azalttı (p=0,0001). Olgun adipositlerde 80 μM quercetin uygulaması, ADP seviyelerinde anlamlı bir artışa yol açtı (p=0,0001). Hipertrofik adipositlere quercetin uygulaması ise, ADP seviyelerinde anlamlı bir azalmaya yol açtı (p=0,0001). Sonuç: Çalışmamız, quercetinin olgun adipositlerde lipit birikimini azaltıp ADP seviyesini artırdığını ortaya koydu. Ancak, hipertrofik adipositlerde ise quercetinin lipit birikiminde anlamlı bir etkisi olmayıp ADP seviyesini azalttığı gözlendi. Bu bulgular, quercetinin obezitenin erken döneminde sağlık üzerindeki koruyucu etkilerini ortaya koymakla birlikte, obezitenin daha ileri döneminde etkinliğinin sınırlı kaldığını göstermektedir.

Keywords

• Adiponektin
• adiposit
• lipit birikimi
• obezite
• quercetin

Investigation of the effect of quercetin on the accumulation of lipids and the level of adiponectin in 3T3-L1 adipocytes

Abstract

Abstract Purpose: Obesity disrupts the homeostasis of adipose tissue, leading to an increase in the number and size of adipose cells. Many positive effects of quercetin polyphenol on health through various mechanisms are known in the literature. The aim of our study was to investigate the effects of quercetin on lipid accumulation and adiponectin (ADP) levels in mature and hypertrophic adipocytes. Materials and methods: In this study, 3T3-L1 differentiated preadipocyte cells were exposed to high glucose media for 8 days to generate mature adipocytes, then for 18 days to produce hypertrophic adipocytes. Quercetin (40 and 80 μM) was administered to mature adipocytes for 24 hours and to hypertrophic adipocytes for 48 hours. Lipid content was visualized by Oil-Red-O staining method. Lipid accumulation and ADP level were measured by ELISA method. For statistical analysis, one-way analysis of variance (post hoc: Tukey test) was used. p≤0.05 was considered statistically significant. Results: The administration of both quercetin doses to mature adipocytes significantly reduced lipid accumulation (p=0.0001). The administration of 80 μM quercetin in mature adipocytes caused a significant increase in ADP levels (p=0.0001). Administration of quercetin to hypertrophic adipocytes caused a significant decrease in ADP levels (p=0.0001). Conclusion: Our study revealed that quercetin decreased lipid accumulation and increased ADP levels in mature adipocytes. However, in hypertrophic adipocytes, quercetin had no significant effect on lipid accumulation and decreased ADP levels. These findings suggest that quercetin has protective effects on health in the early stages of obesity, but its efficacy is limited in the later stages of obesity.

Keywords

• Adiponectin
• adipocyte
• lipid accumulation
• obesity
• quercetin.

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