Investigation of the effect of quercetin on the accumulation of lipids and the level of adiponectin in 3T3-L1 adipocytes
Abstract
Abstract
Purpose: Obesity disrupts the homeostasis of adipose tissue, leading to an increase in the number and size of
adipose cells. Many positive effects of quercetin polyphenol on health through various mechanisms are known
in the literature. The aim of our study was to investigate the effects of quercetin on lipid accumulation and
adiponectin (ADP) levels in mature and hypertrophic adipocytes.
Materials and methods: In this study, 3T3-L1 differentiated preadipocyte cells were exposed to high glucose
media for 8 days to generate mature adipocytes, then for 18 days to produce hypertrophic adipocytes. Quercetin
(40 and 80 μM) was administered to mature adipocytes for 24 hours and to hypertrophic adipocytes for 48
hours. Lipid content was visualized by Oil-Red-O staining method. Lipid accumulation and ADP level were
measured by ELISA method. For statistical analysis, one-way analysis of variance (post hoc: Tukey test) was
used. p≤0.05 was considered statistically significant.
Results: The administration of both quercetin doses to mature adipocytes significantly reduced lipid accumulation
(p=0.0001). The administration of 80 μM quercetin in mature adipocytes caused a significant increase in ADP
levels (p=0.0001). Administration of quercetin to hypertrophic adipocytes caused a significant decrease in ADP
levels (p=0.0001).
Conclusion: Our study revealed that quercetin decreased lipid accumulation and increased ADP levels in
mature adipocytes. However, in hypertrophic adipocytes, quercetin had no significant effect on lipid accumulation
and decreased ADP levels. These findings suggest that quercetin has protective effects on health in the early
stages of obesity, but its efficacy is limited in the later stages of obesity.
Keywords
Adiponectin, adipocyte, lipid accumulation, obesity, quercetin.
Supporting Institution
This study was supported by PAU Scientific Research Projects Coordination Unit (Project No: 2023SABE009).
Ethical Statement
The study is a cell culture study that does not require ethics committee approval.
Thanks
The authors thank Asst. Prof. Hande Senol for her help in statistical analyses.
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