Deneysel diyabet modelinde probiyotiklerin böbrek dokusuna etkisi

Abstract

Amaç: Diyabet, önemli bir halk sağlığı sorunu olan endokrin ve metabolik bir hastalıktır. Tüm dokularda olduğu gibi böbrek dokusunda da hasara yol açmaktadır. Probiyotiklerin yeterli miktarda kullanılması, hastalıkların önlenmesi ve tedavisinde faydalı olabileceği düşünülmektedir. Bu çalışmada, diyabetin böbrek dokusunda oluşturduğu hasara karşı probiyotik takviyesinin etkisini araştırmayı amaçladık. Gereç ve yöntem: Toplam 34 sıçan beş gruba ayrıldı. Kontrol grubuna (K, n=5) PBS, yalnızca probiyotik alan gruba (Pm, n=5) ise probiyotik (0,6 mg/kg) uygulandı. Diyabet grubu (Dm, n=8) streptozotosin (50 mg/kg) ile diyabet oluşturuldu. PmD grubuna (n=8) diyabet oluşturulmadan önce probiyotik takviyesi uygulandı ve diyabet oluştuktan sonra da probiyotik uygulamasına devam edildi. DmP grubunda (n=8) ise önce diyabet oluşturuldu, ardından probiyotik takviyesi uygulandı. Böbrek dokuları histopatolojik ve immünohistokimyasal olarak incelendi; Bcl-2 ve alfa-SMA antikor ekspresyonları değerlendirildi. Bulgular: Kontrol grubunda histopatolojik değişiklikler gözlenmezken, Dm grubunda orta-hafif nefrotoksisite saptandı. Probiyotik takviyesi alan diyabet gruplarında ise hafif nefrotoksisite gözlendi. Dm grubunda Bcl-2 ekspresyonu azalmış olup, DmP ve PmD gruplarında ise daha yüksek seviyelerde olduğu tespit edilmiştir. Alfa-SMA ekspresyonu ise Dm grubunda artarken, DmP ve PmD gruplarında daha düşük seviyelerde olduğu gözlenmiştir. Sonuç: Histopatolojik ve immünohistokimyasal değerlendirmeler, probiyotiklerin diyabetin neden olduğu böbrek hasarına karşı koruyucu bir etki gösterdiğini ortaya koymaktadır.

Keywords

• Diyabet
• probiyotik
• böbrek
• alfa-SMA
• Bcl-2

Effect of probiotics on kidney tissue in an experimental diabetes model

Abstract

Purpose: Diabetes, a major public health issue, is an endocrine and metabolic disease that causes damage to various tissues, including the kidneys. Probiotics are thought to play a beneficial role in the prevention and treatment of diseases when used in sufficient amounts. This study aims to investigate the effects of probiotic supplementation on kidney tissue damage induced by diabetes. Materials and methods: A total of 34 rats were divided into five groups. The control group (K, n=5) received PBS, while the probiotic-only group (Pm, n=5) was given probiotics (0.6 mg/kg). In the diabetes group (Dm, n=8), diabetes was induced using streptozotocin (50 mg/kg). The PmD group (n=8) received probiotic supplementation before diabetes induction, and probiotic administration continued after diabetes was induced. In contrast, the DmP group (n=8) first underwent diabetes induction, followed by probiotic supplementation. Kidney tissues were examined histopathologically and immunohistochemically, with Bcl-2 and alpha-SMA antibody expressions evaluated. Results: No histopathological alterations were observed in the control group. In the Dm group, moderate-to-mild nephrotoxicity was detected, while the probiotic-supplemented diabetes groups exhibited mild nephrotoxicity. Bcl-2 expression was decreased in the Dm group but was found to be higher in the DmP and PmD groups. Conversely, alpha-SMA expression was elevated in the Dm group, whereas it was lower in the DmP and PmD groups. Conclusion: Histopathological and immunohistochemical analyses indicate that probiotics exert a protective effect against diabetes-induced kidney damage.

Keywords

• Diabetes
• probiotic
• kidney
• alpha-SMA
• Bcl-2

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