Does the level of oxidative stress and genotoxicity increase in patients with acute myeloid leukemia?
Abstract
Purpose: The present study evaluates the levels of oxidative stress and DNA damage in patients diagnosed with acute myeloid leukemia (AML; newly diagnosed patients and patients in remission) compared to healthy controls.
Materials and methods: A total of 96 participants were enrolled into this study in three groups: 32 newly diagnosed AML patients, 32 patients in remission, and 32 age- and sex-matched healthy individuals. Various laboratory analyses were conducted to measure biochemical parameters, total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and DNA damage using the comet assay.
Results: We showed that while TOS levels did not differ significantly between the groups, both the newly diagnosed and remission groups exhibited higher OSI levels compared to controls. TAS levels were notably lower in the remission group, suggesting decreased antioxidant capacity. The comet assay demonstrated significantly elevated DNA damage in the newly diagnosed AML group, followed by the remission group, relative to healthy controls. These findings highlight an increased oxidative stress burden and genotoxicity in AML patients, which may contribute to disease pathophysiology. Furthermore, the results suggest that the oxidative stress and DNA damage are more pronounced in newly diagnosed patients compared to those in remission.
Conclusion: The study showed that the oxidative stress and DNA damage observed in AML patients, particularly at diagnosis, could play a role in the progression and recurrence of the disease, suggesting potential prognostic value for oxidative stress markers in AML management.
Keywords
Oxidative stress, acute myeloid leukemia, genotoxicity
Project Number
approval number 60116787-020/59517 at the meeting dated 29/09/2020 and numbered 18
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