Effect of various doses of metformin on map-kinase and apoptosis in penicillin-induced secondary rat cortical astrocytes-can metformin be antiepileptic?

Abstract

Purpose: Astrocytes in the brain and spinal cord perform many functions and it express plasma membrane carriers for various neurotransmitters. Often sclerotic areas in the hippocampus are known as the cause of epilepsy. In reality, astrocytes initiate glutamate release in sclerotic tissue. In experimental epilepsy, penicillin is widely used to initiate this neuronal stress and establish the epilepsy model. The aim of this study is to demonstrate the protective effect of metformin, which has been proven to be effective in neurodegenerative diseases, on penicillin-induced astrocyte degeneration on the primary astrocyte cell line. This study is thought of as the first study about the effect of metformin on penicillin-induced astrocyte death.
Materials and methods: For this purpose, secondary astrocyte cell culture which was previously obtained from the newborn rat brain was used.
Results: The viability of penicillin-induced astrocyte cells was statistically significantly decreased (p=0.01), While cell viability increased statistically significantly in the metformin group at all metformin doses (p=0.01).
Conclusions: We conclude that metformin has protective effects on damage caused by the penicillin secondary astrocyte cell line. These results suggest that penicillin reduces astrocyte cell viability, but metformin protects astrocytes against the lethal effect of penicillin.

Keywords

• Astrocyte
• penicillin
• insulin
• rat
• cell culture

Supporting Institution

Pamukkale Üniversitesi

Project Number

2019TIPF018

Ethical Statement

PAUHADYEK-2019/35 09.10.2019 tarih ve 2019/07 toplantı

Thanks

Pamukkale Üniversitesi Bilimsel Araştırmalar Proje başkanlığına teşekkür

Penisilinle indüklenen sekonder sıçan kortikal astrositlerinde çeşitli metformin dozlarının map-kinaz ve apoptoz üzerine etkisi- metformin antiepileptik olabilir mi?

Abstract

Amaç: Beyin ve omurilikteki astrositler birçok işlevi yerine getirir ve çeşitli nörotransmiterler için plazma membran taşıyıcıları ifade eder. Hipokampustaki sklerotik alanlar sıklıkla epilepsinin nedeni olarak bilinir. Gerçekte, astrositler sklerotik dokuda glutamat salınımını başlatır. Deneysel epilepside, bu nöronal stresi başlatmak ve epilepsi modelini oluşturmak için penisilin yaygın olarak kullanılır. Bu çalışmanın amacı, nörodejeneratif hastalıklarda etkili olduğu kanıtlanmış olan metforminin, birincil astrosit hücre hattında penisilin kaynaklı astrosit dejenerasyonuna karşı koruyucu etkisini göstermektir. Bu çalışma, metforminin penisilin kaynaklı astrosit ölümüne etkisi hakkında yapılan ilk çalışma olarak kabul edilmektedir.
Gereç ve yöntem: Bu amaçla, daha önce yeni doğmuş sıçan beyninden elde edilen ikincil astrosit hücre kültürü kullanılmıştır.

Bulgular: Penisilin kaynaklı astrosit hücrelerinin canlılığı istatistiksel olarak anlamlı derecede azalmıştır (p=0,01). Metformin grubunda ise tüm metformin dozlarında hücre canlılığı istatistiksel olarak anlamlı derecede artmıştır (p=0,01).
Sonuç: Metforminin, penisilin kaynaklı ikincil astrosit hücre hattı hasarına karşı koruyucu etkileri olduğu sonucuna vardık. Bu sonuçlar, penisilinin astrosit hücre canlılığını azalttığını, ancak metforminin astrositleri penisilinin öldürücü etkisine karşı koruduğunu göstermektedir.

Keywords

• Astrosit
• penisilin
• insülin
• sıçan
• hücre kültürü

Project Number

2019TIPF018

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