Kafein sıçan yavrularında bağırsak hasarı gelişiminde predispozan bir risk faktörü mü?

Year 2021, , 338 - 345, 01.04.2021

Özmert M.a. Özdemir , Savaş Saldıray , Yaşar Enli , Nilay Türk , Hacer Ergin

https://doi.org/10.31362/patd.804703

Abstract

Amaç: Hipoksi/reoksijenizasyon (H/R) ile intestinal hasarlanma oluşturulan sıçan yavrularında kafein sitratın histopatolojik ve biyokimyasal etkilerini araştırmak.
Gereç ve yöntem: Bir günlük 32 Wistar albino sıçan yavrusu rastgele dört gruba ayrıldı: kontrol grubu (grup1, n=8), kafein grubu (grup2, subkutan kafein sitrat uygulanan, n=8), H/R grubu (grup3, H/R uygulanan, n=8) ve kafein+H/R grubu (grup4, kafein sitrat verilen ve H/R uygulanan, n=8). Kafein sitrat, 20 mg/kg'lık bir yükleme dozunda başlatıldı, ardından subkutan 5 mg/kg/günlük idame dozu takip edildi. Dördüncü günde, grup 1 ve 2 dışındaki tüm hayvanlar H/R'ye maruz bırakıldı ve H/R prosedüründen 6 saat sonra öldürüldü.
Histopatolojik hasarlanma skorları (HIS), interlökin-6 (IL-6), tümör nekroz faktörü-alfa (TNF-α) ve oksidatif stres indeksi (OSI: toplam oksidan durum “TOS”/toplam antioksidan durum “TAS”) seviyeleri bağırsak örnekleri üzerinden değerlendirildi.
Bulgular: Histopatolojik olarak en ciddi hasar H/R uygulanan gruplarda görüldü (p <0.01). Kafein grubunun ortalama HIS'leri kontrol grubundan daha yüksek iken, H/R grubundan daha düşüktü, ancak bu istatistiksel olarak anlamlı değildi. Grup 2, 3 ve 4'teki TNF-α, IL-6 ve OSI düzeyleri kontrol grubundan anlamlı olarak yüksekti (p <0.05). Bununla birlikte, kafein grubundaki bu biyokimyasal parametreler, H/R uygulanan gruplardan anlamlı olarak daha düşüktü (p <0.01).
Sonuç: Bu çalışma, kafein sitratın TNF-α, IL-6 ve OSI'nin bağırsak doku düzeylerini önemli ölçüde arttırdığını gösterdi. Bu nedenle, kafeinin bağırsak hasarı geliştirmek için predispozan bir risk faktörü olabileceğini düşünüyoruz.

Keywords

Kafein, intestinal hasarlanma, yenidoğan

Supporting Institution

Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Kurulu

Project Number

2017TPF011

Thanks

Yazarlar olarak Barbaros Şahin ve Pamukkale Üniversitesi Hayvan Araştırmaları Laboratuvarı'na yardımları için çok teşekkür ederiz

Is caffeine a predisposing risk factor for developing intestinal injury in newborn rats?

Abstract

Purpose: To investigate of histopathologic and biochemical effects of caffeine citrate in newborn rats with hypoxia/reoxygenation (H/R)-induced intestinal injury.
Materials and methods: One-day-old, 32 Wistar albino newborn rats (n=8) were randomly divided into four groups: control group (group1, n=8), caffeine group (group2, caffeine citrate administered subcutaneously, n=8), H/R group (group3, exposed to H/R, n=8), and caffeine + H/R group (group4, caffeine citrate administered and exposed to H/R, n=8). Caffeine citrate was initiated at a loading dose of 20 mg/kg, followed by a maintenance dose of 5 mg/kg/day, subcutaneously. On day 4th, all animals except for groups 1 and 2 were exposed to H/R and sacrificed 6 hours after H/R procedure. Histopathological injury scores (HISs), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and oxidative stress index (OSI: total oxidant status “TOS”/total antioxidant status “TAS”) levels were measured in intestinal samples.
Results: As histopathological, the most severe damage was observed in H/R-induced groups (p<0.01). Although not statistically significant, the mean HISs of caffeine group was higher than the control group and lower than the H/R group. The levels of TNF-α, IL-6 and OSI in the groups 2, 3 and 4 were significantly higher than the control group (p<0.05). However, these biochemical parameters in the caffeine group were significantly lower than those of the H/R-induced groups (p<0.01).
Conclusion: This study showed that caffeine citrate significantly increased the intestinal tissue levels of TNF-α, IL-6, and OSI. As a result, caffeine may be a predisposing risk factor for developing intestinal injury.   

Keywords

Caffeine, intestinal injury, newborn

Project Number

2017TPF011

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