Ketamıne/xylazine anesthesia ıs safe in hemorheological point of view: a preliminary report

Abstract

Purpose: Some anesthetic drugs are known to have influence on circulation due to hemorheological effects. We aimed to investigate the possible effects of ketamin-xylazine anesthesia, which is extensively used in experimental animal laboratories on hemorheological parameters. Materials and methods: Adult, male Wistar-Albino rats were divided into two as control (pre-anesthesia) and post-anesthesia (n=17). Ketamin-HCl/Xylazine-HCl (90 mg/kg-10 mg/kg) were injected intraperitoneally. Intracardiac heparinized blood was collected either without anesthesia (control group) or after anesthesia (post-anesthesia group) following the loss of reflexes such as righting, cornea and withdrawal. Erythrocyte deformability was determined by an ektacytometer, while WBV (at shear rates of 38, 76 and 190 s–1) and PV (at 190 s–1 shear rate) at standard (40%) and otolog hematocrit (Hct) were measured via a cone-plate rotational viscometer. Results: Mean time taken for the disappearance of righting reflex was 4 ± 3.6, corneal reflex 8 ± 6.8 and for withdrawal reflex 10 ± 6.7 min. 90 mg/kg Ketamin-10 mg/kg Xylazine administration did not affect RBC deformability, WBV and PV of the rats. Conclusion: These results demonstrate that, use of Ketamin/Xylazine anesthesia may be recommended especially when dealing with hemorheological, cardiovascular and hematological parameters.

Keywords

• ketamin/xylazine
• anesthesia
• erythocyte deformability
• viscosity
• rat

Ketamin/ksilazin anestezi hemoreolojik bakış açısından güvenlidir: bir ilk rapor

Öz

Amaç: Bazı anestezik ilaçların hemoreolojik etkileri nedeniyle dolaşımı etkilediği bilinmektedir. Deney hayvan laboratuarlarında yaygın olarak kullanılan ketamin-ksilazin anestezisinin hemoreolojik parametreler üzerine olası etkilerini araştırmayı amaçladık. Gereç ve yöntem: Yetişkin, erkek Wistar-Albino sıçanlar kontrol (anestezi öncesi) ve anestezi sonrası (n = 17) olarak ikiye ayrıldı. Ketamin-HCl / Xylazine-HCl (90 mg / kg-10 mg / kg) intraperitoneal olarak enjekte edildi. İntrakardiyak heparinize kan anestezi olmadan alındı ​​(kontrol grubu) veya anestezi sonrası (anestezi sonrası grup) düzeltme, kornea ve geri çekme gibi reflekslerin kaybını takiben. Eritrosit deformabilitesi bir ektasitometre ile belirlendi, WBV ise (38, 76 ve 190 s – 1 kesme hızlarında) ve PV (190 sn – 1 kesme hızında) standartta (% 40) ve otolog hematokrit (Hct) bir koni plakalı rotasyonel viskometre ile ölçülmüştür. Bulgular: Doğrultma refleksinin kaybolması için alınan ortalama süre 4 ± 3.6, kornea refleksi 8 ± 6.8 ve geri çekilme refleksi 10 ± 6.7 dakika idi. 90 mg / kg Ketamin-10 mg / kg Xylazine uygulaması, sıçanların RBC deforme olabilirliğini, WBV ve PV'sini etkilememiştir. Sonuç: Bu sonuçlar, Ketamin / Xylazine anestezisinin özellikle hemoreolojik, kardiyovasküler ve hematolojik parametrelerle uğraşırken önerilebileceğini göstermektedir.

Anahtar Kelimeler

• ketamin/ksilazin
• anestezi
• eritrosit deformobilite
• viskozite
• sıçan

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