N-asetilsistein ve ozon gibi antioksidan ilaçların staz zonu üzerindeki etkinliği

Öz

Amaç: Pıhtılaşma bölgesi etrafındaki bölge olan durağanlık bölgesi, yanıklarda nekrozun önlenmesi için önemlidir. Literatürde staz bölgesinde nekrozu önlemek için çeşitli ilaçlar çalışılmıştır. Önceki araştırmalar, termal yaralanmada N-asetilsistein tedavisinin hem uzak organlarda hem de lokal tarafta etkinliğini göstermiştir. Buna bağlı olarak, N-asetilsistein ve Ozonun termal yaralanma durağan bölgesi önleme etkinliğinde gösterilmesi amaçlanmıştır.
Yöntem: Bu amaçla çalışmada, ağırlıkları 200±20 g olan 24 adet 4 aylık Wistar albino tipi dişi sıçan kullanıldı. Bu sıçanlarda bileşik yanma modeli yapıldı. Sonra N-asetilsistein ve Ozonun etkililiğini karşılaştırmak için bileşik yanma modeline uygulandı. Histopatolojik yıkım şiddetini belirlemek için bir skorlama yöntemi uygulandı. Oksidasyon ve antioksidasyon sistemi arasındaki dengede meydana gelen değişiklikleri saptamak için sıçanların serum örneklerinde Malondialdehid (MDA), Glutatyon Perpxidaz (GPx), Superoksid dismutaz (SOD) ve katalaz (CAT) seviyeleri analiz edildi.
Bulgular: SOD düzeyleri grup III'te grup II'ye göre anlamlı olarak yüksekti (p<0,05). CAT seviyeleri grup II ve III'te grup I'den daha yüksekti (p<0,001, p<0,001). GPx düzeyleri grup II (p<0,001) ve grup III (p<0,001)'de daha yüksekti. Histopatolojik skorlar Grup III'de grup I'den daha düşüktü (p=0,001).
Sonuç: Bu çalışmada, hem Ozon hem de N-asetilsisteinin, staz bölgesinin kurtarılmasında etkili ajanlar olduğunu göstermiştir. Ayrıca Ozon, N-asetilsisteinden daha faydalı olabileceği sonucuna varılmıştır.

Anahtar Kelimeler

• Yanık
• staz bölgesi
• antioksidan
• ozon
• oksidan.

The effectiveness of antioxidant drugs such as N-acetylcysteine and Ozone on the stasis zone

Abstract

Title: The effectiveness of antioxidant drugs such as N-acetylcysteine and Ozone on the stasis zone.
Short title: Effectiveness of N-acetylcysteine and Ozone on the stasis zone.
Purpose: Stasis zone, the zone around the coagulation zone, is important for the prevention necrosis in burns. Various drugs were studied in literature to prevent necrosis in stasis zone. The previous researches have shown the effectiveness of Nacetylcysteine treatment on both remote organs and local side in thermal injury. Demonstration of the effectiveness of N acetylcysteine and Ozone on stasis zone prevention in thermal injury was the goal of this study.
Methods: For this purpose, 24 4-month-old Wistar albino type female rats weighing 200±20 g were used in the study. Compound burning model was made in these rats. Next, the compound was applied to the combustion model to compare the effectiveness of N-acetylcysteine and Ozone. To identfy histopathological destruction severity a scoring method was applied. Malondialdhyede (MDA), Glutathione Peroxidase (GPx), Superoksid dismutase (SOD), and catalase (CAT) levels were analyzed in serum samples of the rats to find out the alterations in the balance between oxidatitaion and antioxidation system.
Results: While the highest MDA levels were observed in group I, the decrease was significant in group II and III (p=0.03 and p<0.02). SOD levels were significantly higher in group III compared to group II (p<0.05). CAT levels were higher in group II and III than group I (p<0.001, p<0.001). GPx levels were higher in group II (p<0.001), and group III (p<0.001). Histopathological scores were lower in group III than group I (p=0.001).
Conclusion: This study showed that both Ozone and N-acetylcysteine are effective agents in rescuing the stasis site. It was also concluded that Ozone might be more beneficial than N-acetylcysteine.

Keywords

• Burn
• zone of stasis
• antioxidan
• ozone
• oxidant

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