Taraxacum officinale Kök Ekstraktının Antioksidan Potansiyeli ve Fenolik İçeriği

Abstract

Amaç: Bu çalışmanın amacı, potansiyel terapötik kullanımla sahip, tıbbi bir bitki olan Taraxacum officinale köklerinin antioksidan kapasitesini ve biyoaktif kimyasal profilini değerlendirmektir. Yöntemler: Köklern ekstraksiyonu için %70 metanol kullanılmış ve ardından çözücü buharlaştırılmıştır. Hem toplam flavonoid içeriği (TFC) hem de toplam fenolik içerik (TPC) ölçülmüştür. Antioksidan kapasitesini ölçmek için DPPH, ABTS ve FRAP testleri kullanılmıştır. Sonuçlar: DPPH ve ABTS analizleri için IC50 değerleri sırasıyla 50,47±0,41 µg/mL ve 70,57±0,44 µg/mL olarak tespit edilmiştir. FRAP analizi ile ölçülen indirgeyici güç, 26,87 ± 0,12 mg Trolox eşdeğeri/g özüt olarak belirlenmiştir. TPC 23,58 ± 0,97 mg gallik asit eşdeğeri/g özüt ve TFC 7,76 ± 0,81 mg kuersetin eşdeğeri/g özüt olarak bulunmuştur. Sonuç: Taraxacum officinale kök özütünün yüksek fenolik ve flavonoid içeriği, orta derecede antioksidan aktivitesini açıklamaktadır. Bu bulgular, farmasötik ve nutrasötik endüstrilerinde doğal bir antioksidan kaynağı olarak potansiyel kullanımını göstermektedir.

Keywords

• ABTS
• DPPH
• FRAP
• Taraxacum officinale (L.) Weber ex F.H. Wigg

Antioxidant Potential and Phenolic Content of Taraxacum officinale Root Extract

Abstract

Objective: The purpose of this study was to assess the antioxidant capability and bioactive chemical profile of Taraxacum officinale roots, a medicinal plant with potential therapeutic uses. Methods: 70% methanol was used to extract the roots, and the solvent was then evaporated. Both the total flavonoid content (TFC) and the total phenolic content (TPC) were measured. The DPPH, ABTS, and FRAP tests were used to measure antioxidant capability. Results: The IC50 values for DPPH and ABTS assays were 50.47±0.41 µg/mL and 70.57±0.44 µg/mL, respectively. The reducing power, measured by the FRAP assay, was 26.87 ± 0.12 mg Trolox equivalents/g extract. The TPC was 23.58 ± 0.97 mg gallic acid equivalents/g extract, and the TFC was 7.76 ± 0.81 mg quercetin equivalents/g extract. Conclusion: Taraxacum officinale root extract's high phenolic and flavonoid content contributes to explain its modest antioxidant activity. These findings indicate its potential use as a natural antioxidant source in the pharmaceutical and nutraceutical industries.

Keywords

• ABTS
• DPPH
• FRAP
• Taraxacum officinale (L.) Weber ex F.H. Wigg

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