Effect of Saxagliptin on Oxidative Stress Parameters in Diabetes-Induced Testicular Damage

Abstract

In our study, we aimed to investigate the effects and possible mechanisms of the Dipeptidyl peptidase-4 (DPP-4) inhibitor saxagliptin (Sax) on streptomizole (STZ)-induced testicular damage in rats. The animals were allocated into four groups: Control, Sax, DM, and DM+Sax. Oxidative stress was evaluated by measuring MDA, AOPP, IMA and nitrate-nitrite levels. Antioxidant parameters, including GSH, ceruloplasmin levels and activities of SOD, CAT, and GPx, were assessed using the specified methods. In this study, the protective effects of saxagliptin against diabetes-induced testicular damage were evaluated by analysis of oxidative and nitrosative stress markers including SOD, CAT, GSH, ceruloplasmin, MDA, and nitrite/nitrate levels. Diabetes significantly decreased SOD, CAT, and GSH activities, while increased MDA, nitrite, and nitrate levels, indicating increased oxidative and nitrosative stress. Saxagliptin treatment restored SOD and CAT activities, increased GSH levels, and normalized ceruloplasmin concentrations, reflecting its capacity to support the antioxidant defense system. In addition, saxagliptin significantly decreased MDA levels and nitrite/nitrate concentrations, indicating its efficacy in reducing lipid peroxidation and nitrosative stress. Saxagliptin has demonstrated a protective effect against streptozotocin (STZ)-induced testicular damage by reducing oxidative stress and enhancing the antioxidant defense system. Studies suggest that saxagliptin can mitigate oxidative injury in testicular tissue, supporting cellular health through increased antioxidant enzyme activity in STZ-induced models.

Keywords

• Antioxidant
• Diabetes mellitus Oxidative stress
• Saxagliptin
• Testicular toxicity

Saxagliptin’in Diyabet İndüklenmiş Testiküler Hasarda Oksidatif Stres Parametreleri Üzerine Etkisi

Abstract

Bu çalışmada, Dipeptidil peptidaz-4 (DPP-4) inhibitörü olan saxagliptin’in (Sax), streptozotosin (STZ) ile oluşturulan testiküler hasar üzerindeki etkilerini ve olası mekanizmalarını araştırmayı amaçladık. Hayvanlar dört gruba ayrıldı: Kontrol, Sax, DM (Diyabet), ve DM+Sax. Oksidatif stres; MDA, AOPP, İMA ve nitrat-nitrit düzeylerinin ölçülmesiyle değerlendirildi. GSH, seruloplazmin düzeyleri ile SOD, CAT ve GPx enzim aktiviteleri gibi antioksidan parametreler de belirli yöntemlerle analiz edildi. Bu çalışmada saxagliptin’in diyabete bağlı testiküler hasara karşı koruyucu etkileri, SOD, CAT, GSH, seruloplazmin, MDA ve nitrit/nitrat gibi oksidatif ve nitrozatif stres belirteçleri üzerinden değerlendirildi. Diyabetin, SOD, CAT ve GSH aktivitelerini anlamlı şekilde azalttığı; MDA, nitrit ve nitrat düzeylerini ise artırdığı ve bu durumun artmış oksidatif ve nitrozatif stresi gösterdiği belirlendi. Saxagliptin tedavisi, SOD ve CAT aktivitelerini geri kazandırdı, GSH düzeylerini artırdı ve seruloplazmin konsantrasyonlarını normalize ederek antioksidan savunma sistemini desteklediğini gösterdi. Ayrıca, saxagliptin MDA ile nitrit/nitrat düzeylerini anlamlı ölçüde azaltarak, lipid peroksidasyonu ve nitrozatif stresin azalmasına katkıda bulundu. Saxagliptin’in STZ ile indüklenmiş testiküler hasara karşı oksidatif stresi azaltarak ve antioksidan savunma sistemini güçlendirerek koruyucu etki gösterdiği ortaya konmuştur. Yapılan çalışmalar saxagliptin’in testiküler dokudaki oksidatif hasarı hafifletebileceğini ve STZ ile oluşturulan modellerde antioksidan enzim aktivitelerini artırarak hücresel sağlığı desteklediğini göstermektedir.

Keywords

• Antioksidan
• Diabetes mellitus
• Oksidatif stres
• Saxagliptin
• Testiküler toksisite

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