Effects of Duloxetine on Oxidant-Antioxidant System in Rat Brain Tissues

Year 2024, , 16 - 21, 01.03.2024

Kadir Karakuş , Kadir Demirci , Efkan Uz , Ayşe Yiğit , Ramazan Özcankaya

https://doi.org/10.38175/phnx.1383064

Abstract

Objective: After the relationship between depression and oxidative stress (OS) was demonstrated, the effect of antidepressant drugs on OS has become important. In this study, we aimed to determine the effects of the antidepressant duloxetine on the activities of the superoxide dismutase (SOD), catalase (CAT), adenosine deaminase (ADA), xanthine oxidase (XO) and glutathione peroxidase (GSH-Px) enzymes as well as the lipid peroxidation (LP) product malondialdehyde (MDA) and nitric oxide (NO) levels in rat brains.
Material and Method: Twenty male Sprague-Dawley rats were used for the study. The first group was the control group (n=10) and the second group was the duloxetine group (n=10). Duloxetine was administered intragastrically once a day at a dose of 10 mg/kg for two weeks in the second group. Water was administered intragastrically once a day for two weeks in the first group. Rats were sacrificed at the end of the fourteenth day. The brain tissues were collected and then analyzes were performed.
Results: As a result of this study, we found that duloxetine increased the SOD (P=0.026) activity and decreased the ADA (P=0.041), XO (P=0.034) and CAT (P=0.006) activities significantly compared to the control group. We also found an increase in the GSH-Px enzyme activity and decrease in the NO and MDA levels at non-significant rates in the duloxetine group brain tissues.
Conclusion: The significant increase in the activity of the antioxidant enzyme SOD, the significant decrease in the activities of the XO and ADA enzymes, which can cause the formation of reactive oxygen products in the organism, and the insignificant decrease in the LP indicator MDA suggest that duloxetine can positively change the antioxidant status in rat brain tissues.

Keywords

Adenosine deaminase, Xanthine oxidase, Superoxide dismutase, Catalase, Malondialdehyde, Duloxetine

Ethical Statement

Çıkar Çatışması: Tüm yazarlar çıkar çatışması olmadığını beyan ettiler. Etik: Çalışma Süleyman Demirel Üniversitesi Tıp Fakültesi Hayvan Deneyleri Yerel Etik Kurulundan onay alınarak (27.05.2010 tarih ve 04 sayılı karar) etik kurul kurallarına uygun bir şekilde yapılmıştır.

Project Number

Bu çalışma Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi tarafından 2183-TU-10 Proje numarası ile desteklenmiştir.

Duloksetinin Rat Beyin Dokularındaki Oksidan-Antioksidan Sistem Üzerine Etkisi

Abstract

Amaç: Oksidatif stres (OS) ve depresyon arasındaki ilişki gösterildikten sonra antidepresanların OS üzerine etkisi önemli hale gelmiştir. Bu çalışmanın amacı duloksetinin rat beyin dokularındaki katalaz (CAT), süperoksit dismutaz (SOD), adenozin deaminaz (ADA), ksantin oksidaz (XO) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri ile nitrik oksit (NO) ve malondialdehid (MDA) düzeylerine etkilerini araştırmaktır.
Gereç ve Yöntem: Çalışmaya birinci grup kontrol grubu (n=10) ve ikinci grup duloksetin grubu (n=10) olmak üzere toplam yirmi tane Sprague-Dawley cinsi erkek rat alındı. Duloksetin grubuna günde bir defa 10 mg/kg dozunda intragastrik yoldan iki hafta süreyle duloksetin verildi. Kontrol grubuna da günde bir defa iki hafta süreyle intragastrik olarak su verildi. On beşinci günde tüm ratlar sakrifiye edilerek beyin dokuları çıkarıldı ve incelemeler yapıldı.
Bulgular: Çalışmamızda duloksetinin rat beyin dokularında kontrol grubuna kıyasla SOD (P=0,026) enzim aktivitesini anlamlı düzeyde artırdığını ve XO (P=0,034), ADA (P=0,041) ve CAT (P=0,006) enzim aktivitelerini anlamlı düzeyde azalttığını saptadık. Ayrıca GSH-Px enzim aktivitesini anlamlı olmayan düzeyde artma ve MDA ile NO düzeylerinde ise anlamlı olmayan düzeyde azalma saptadık.
Sonuç: Çalışmamızda antioksidan bir enzim olan SOD enzim aktivitesinin anlamlı düzeyde artması, organizmada reaktif oksijen ürünleri oluşumuna neden olabilen XO ve ADA enzim aktivitelerinin anlamlı düzeyde azalması ve LP göstergesi olan MDA’nın artmayıp, anlamlı düzeyde olmasa da azalması duloksetinin antioksidan durumu olumlu yönde değiştirebileceğini düşündürmektedir.

Keywords

Duloksetin, Adenozin deaminaz, Ksantin oksidaz, Süperoksit dismutaz, Katalaz, Malondialdehit

Project Number

Bu çalışma Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi tarafından 2183-TU-10 Proje numarası ile desteklenmiştir.

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