Association of IL2-330 Gene Polymorphism with Lung Cancer

Abstract

Cytokines are secreted or membrane-bound proteins that act as mediators of intercellular signaling to regulate homeostasis of the immune system. They are produced by cells of innate and adaptive immunity in response to microbes and tumor antigens. Although there are several studies showing that IL2-330 gene polymorphism is associated with many types of cancer, as far as we know, there is a few study investigating the association between lung cancer and IL2-330 gene polymorphism. In this study, the role of IL2-330 gene polymorphism in the pathogenesis of lung cancer was investigated. 96 patients who were diagnosed with lung cancer and 96 age and sex matched healthy subjects participated in the study. Genomic DNA was isolated using the blood DNA isolation kit and the IL2-330 gene polymorphism was determined by polymerase chain reaction-confronting two pairs primer method. When analyzed for the lung cancer group and the healthy group according to IL2-330 gene polymorphism, genotype and allele frequencies were found to be similar in both groups (p>0,05). As a result; there was no statistically significant difference between the groups. Considering the ethnic diversity of lung cancer, the study needs verified in other populations.

Keywords

• Cytokines
• Interleukine 2
• IL2
• Lung cancer
• Polymorphism

IL2-330 Gen Polimorfizminin Akciğer Kanseri ile İlişkisinin Araştırılması

Abstract

Sitokinler, bağışıklık sisteminin homeostazını düzenlemek için hücreler arası sinyalleşmenin aracıları olarak görev yapan, salgılanan veya zara bağlı proteinlerdir. Mikroplara ve tümör antijenlerine yanıt olarak doğuştan gelen bağışıklığa sahip hücreler tarafından üretilirler. IL2-330 gen polimorfizminin birçok kanser türü ile ilişkili olduğunu gösteren birkaç çalışma olmasına rağmen, bildiğimiz kadarıyla akciğer kanseri ile IL2-330 gen polimorfizmi arasındaki ilişkiyi araştıran az sayıda çalışma vardır. Bu çalışmada IL2-330 gen polimorfizminin akciğer kanseri patogenezindeki rolü araştırılmıştır. Çalışmaya akciğer kanseri tanısı almış 96 hasta ile yaş ve cinsiyet bakımından uyumlu 96 sağlıklı, gönüllü birey katıldı. Genomik DNA izolasyon kiti kullanılarak izole edildi ve IL2 -330 gen polimorfizmi polimeraz zincir reaksiyonu ile iki çift primer yöntemiyle karşılaştırılarak belirlendi. Akciğer kanserinde IL2-330 gen polimorfizmine göre hasta grubu ve sağlıklı grup incelendiğinde genotip ve allel frekanslarının her iki grupta benzer olduğu görüldü (p>0,05). Sonuç olarak gruplar arasında istatistiksel olarak anlamlı bir fark yoktu. Akciğer kanserinin etnik çeşitliliği göz önüne alındığında, çalışmanın diğer popülasyonlarda da doğrulanması gerekmektedir.

Keywords

• Sitokin
• Interlökin 2
• IL2
• Akciğer kanseri
• Polimorfizm

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