Year 2021, Volume 3 , Issue 2, Pages 81 - 84 2021-07-01

Association of IL2-330 Gene Polymorphism with Lung Cancer
IL2-330 Gen Polimorfizminin Akciğer Kanseri ile İlişkisinin Araştırılması

Öyküm GENÇ [1] , Erdi AKAR [2] , Erkan ARPACI [3] , Hüseyin ENGİN [4] , Sevim KARAKAŞ ÇELİK [5]


Cytokines are secreted or membrane-bound proteins that act as mediators of intercellular signaling to regulate homeostasis of the immune system. They are produced by cells of innate and adaptive immunity in response to microbes and tumor antigens. Although there are several studies showing that IL2-330 gene polymorphism is associated with many types of cancer, as far as we know, there is a few study investigating the association between lung cancer and IL2-330 gene polymorphism. In this study, the role of IL2-330 gene polymorphism in the pathogenesis of lung cancer was investigated. 96 patients who were diagnosed with lung cancer and 96 age and sex matched healthy subjects participated in the study. Genomic DNA was isolated using the blood DNA isolation kit and the IL2-330 gene polymorphism was determined by polymerase chain reaction-confronting two pairs primer method. When analyzed for the lung cancer group and the healthy group according to IL2-330 gene polymorphism, genotype and allele frequencies were found to be similar in both groups (p>0,05). As a result; there was no statistically significant difference between the groups. Considering the ethnic diversity of lung cancer, the study needs verified in other populations.
Sitokinler, bağışıklık sisteminin homeostazını düzenlemek için hücreler arası sinyalleşmenin aracıları olarak görev yapan, salgılanan veya zara bağlı proteinlerdir. Mikroplara ve tümör antijenlerine yanıt olarak doğuştan gelen bağışıklığa sahip hücreler tarafından üretilirler. IL2-330 gen polimorfizminin birçok kanser türü ile ilişkili olduğunu gösteren birkaç çalışma olmasına rağmen, bildiğimiz kadarıyla akciğer kanseri ile IL2-330 gen polimorfizmi arasındaki ilişkiyi araştıran az sayıda çalışma vardır. Bu çalışmada IL2-330 gen polimorfizminin akciğer kanseri patogenezindeki rolü araştırılmıştır. Çalışmaya akciğer kanseri tanısı almış 96 hasta ile yaş ve cinsiyet bakımından uyumlu 96 sağlıklı, gönüllü birey katıldı. Genomik DNA izolasyon kiti kullanılarak izole edildi ve IL2 -330 gen polimorfizmi polimeraz zincir reaksiyonu ile iki çift primer yöntemiyle karşılaştırılarak belirlendi. Akciğer kanserinde IL2-330 gen polimorfizmine göre hasta grubu ve sağlıklı grup incelendiğinde genotip ve allel frekanslarının her iki grupta benzer olduğu görüldü (p>0,05). Sonuç olarak gruplar arasında istatistiksel olarak anlamlı bir fark yoktu. Akciğer kanserinin etnik çeşitliliği göz önüne alındığında, çalışmanın diğer popülasyonlarda da doğrulanması gerekmektedir.
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Primary Language en
Subjects Genetics and Heredity, Medicine, Immunology
Journal Section Research Articles
Authors

Orcid: 0000-0002-9805-2663
Author: Öyküm GENÇ (Primary Author)
Institution: İSTANBUL ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0003-2774-9452
Author: Erdi AKAR
Institution: ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-4298-340X
Author: Erkan ARPACI
Institution: ÖZEL ADATIP SAKARYA HASTANESİ
Country: Turkey


Orcid: 0000-0001-6444-504X
Author: Hüseyin ENGİN
Institution: ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Country: Turkey


Orcid: 0000-0003-0505-7850
Author: Sevim KARAKAŞ ÇELİK
Institution: ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Country: Turkey


Dates

Application Date : March 1, 2021
Acceptance Date : June 8, 2021
Publication Date : July 1, 2021

AMA Genç Ö , Akar E , Arpacı E , Engin H , Karakaş Çelik S . Association of IL2-330 Gene Polymorphism with Lung Cancer. Phnx Med J.. 2021; 3(2): 81-84.